Sodium phosphate dibasic

Manufactured by Thermo Fisher Scientific

Sourced in United States, United Kingdom, Belgium, Spain

Sodium phosphate dibasic is a chemical compound with the formula Na2HPO4. It is a white, crystalline solid that is commonly used as a buffer, pH adjuster, and electrolyte in various laboratory applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

Sodium phosphate (37 citations) Sodium phosphate dibasic anhydrous (31 citations) Sodium phosphate dibasic heptahydrate (17 citations) Sodium phosphate dibasic (Na2HPO4) (11 citations) Monobasic and dibasic sodium phosphate (2 citations) Sodium phosphate dibasic heptahydrate (Na2HPO4•7H2O) (2 citations) Dibasic sodium hydrogen phosphate (2 citations)

51 protocols using sodium phosphate dibasic

Development of Nicotine-Loaded Buccal Strips

Check if the same lab product or an alternative is used in the 5 most similar protocols

Hydroxypropylmethylcellulose (HPMC; Methocel K100 Premium LV) was a gift from Colorcon Limited (Dartford, UK), and magnesium aluminium silicate (MAS) was a gift from R.T. Vanderbilt Company Inc. (Norwalk, CT, USA). Sodium hydroxide, potassium dihydrogen phosphate, and gelatine were purchased from Fluka Analytical (Buchs, Switzerland). Nicotine (liquid form), sodium alginate (SA; molecular weight 120,000–190,000 g/mol, mannuronate/guluronate ratio 1.56), submaxillary mucin from porcine stomach, PBS tablets (pH 7.4), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Krebs–Ringer bicarbonate buffer, and dimethyl sulfoxide (DMSO) were all obtained from Sigma Aldrich (Dorset, UK). Sodium acetate, trimethylamine, and glycerol (GLY) were purchased from Fisher Scientific (Loughborough, UK). Commercially available NIC-loaded strips (NiQuitin^®) was purchased from a local pharmacy (Gillingham, Kent). Calcium chloride dehydrate, sodium chloride, sodium phosphate dibasic, magnesium chloride hexahydrate, potassium carbonate hemihydrate, sodium phosphate monobasic monohydrate, Sodium acetate, and trimethylamine were all purchased from Fisher Scientific (Loughborough, UK). The EpiOral^TM buccal tissue kit (ORL-200) was purchased from MatTek Corporation (Ashland, MA, USA).

Boateng J, & Okeke O. (2019). Evaluation of Clay-Functionalized Wafers and Films for Nicotine Replacement Therapy via Buccal Mucosa. Pharmaceutics, 11(3), 104.

+ Open protocol

+ Expand

Comprehensive Analytical Reagent Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Sodium azide, sodium bromide, sodium chloride, potassium fluoride, potassium nitrate, sodium phosphate dibasic, sodium silicate, sodium sulfate, and sodium thiosulfate were obtained from Fisher Scientific (Fairlawn, NJ, USA). Sodium acetate, sodium arsenate, sodium carbonate, and potassium nitrite were purchased from J.T. Baker (Phillipsburg, NJ, USA). Lactic acid was acquired from Eastman (Kingsport, TN, USA). All reagents were of analytical grade. Ultra-pure CE water procured from Agilent Technologies (Santa Clara, CA, USA) from their Forensic Anion Solution Kit (PN 5064-8208).

Varden L., Smith B, & Bou-Abdallah F. (2017). Detection and Quantification of Inorganic and Organic Anions in Natural, Potable, and Wastewaters in Northern New York Using Capillary Zone Electrophoresis and Indirect UV Detection. Journal of chromatography & separation techniques, 8(2), 361.

+ Open protocol

+ Expand

Bovine Erythrocyte CA Biosensor Fabrication

Check if the same lab product or an alternative is used in the 5 most similar protocols

CA from bovine erythrocytes,
TBAB, p-nitrophenyl acetate, Nafion, acetonitrile,
poly(ethylene
glycol) diglycidyl ether, aniline, 3-aminobenzoic acid, glutaraldehyde,
and ammonium sulfate were purchased from Sigma (St Louis, MO, USA).
Sodium phosphate monobasic was purchased from Macron and sodium phosphate
dibasic from Fisher Chemicals.

Grattieri M., Hickey D.P., Kim H.S., Seijas V.T., Kim J, & Minteer S.D. (2018). Lag Time Spectrophotometric Assay for Studying Transport Limitation in Immobilized Enzymes. ACS Omega, 3(9), 11945-11949.

+ Open protocol

+ Expand

Kinetics Studies Solution Preparation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phosphate buffers were prepared using sodium phosphate monobasic, sodium phosphate dibasic, and sodium chloride from Fisher Scientific. The MOPS (3-(N-morpholino)propanesulfonate) buffer was purchased from EMD while Krebs buffer was purchased from Sigma Aldrich. Nanopure water (≥18 megohm) was obtained from a Barnstead Nanopure II system and used in solution preparations for kinetics studies.

DeMartino A.W., Souza M.L, & Ford P.C. (2017). Uncaging carbon disulfide. Delivery platforms for potential pharmacological applications: a mechanistic approach †Electronic supplementary information (ESI) available: Additional documentation (10 tables, and 14 figures) including crystallographic parameters, modifications of published synthetic procedures and development of models to fit the kinetics effects of varying buffer concentration. (50 pages total, PDF). Separate Supporting Information files are the X-ray crystallographic data in CIF format. CCDC 1557060–1557062. For ESI and crystallographic data in CIF or other electronic format see DOI: 10.1039/c7sc02727c Click here for additional data file. Click here for additional data file.. Chemical Science, 8(10), 7186-7196.

+ Open protocol

+ Expand

Quantification of Betacyanins, Betaxanthins, and Phenolics

Check if the same lab product or an alternative is used in the 5 most similar protocols

Ethanol (purity > 99%), citric acid (purity > 95%), sodium hydroxide pellets, sodium phosphate dibasic (purity ≥ 99%), formic acid (Purity ≥ 98%), acetonitrile (LC/MS grade) and sodium carbonate were supplied by Fisher Scientific (Loughborough, UK). Folin–Ciocalteau reagent (2 M) was purchased from Scientific Laboratory Supplies Ltd. (Nottingham, UK). Standards of betanin (purity > 99%) and gallic acid (purity ≥ 98%) to measure betacyanin, betaxanthin and total phenolic content were purchased from Merck Chemicals Limited (Dorset, UK).

Kumar R., Methven L, & Oruna-Concha M.J. (2023). A Comparative Study of Ethanol and Citric Acid Solutions for Extracting Betalains and Total Phenolic Content from Freeze-Dried Beetroot Powder. Molecules, 28(17), 6405.

+ Open protocol

+ Expand

Topical Delivery of Tofacitinib

Check if the same lab product or an alternative is used in the 5 most similar protocols

Tofacitinib citrate was purchased from CSN Pharm (Arlington Heights, IL, U.S.A). Phosphate buffered saline, pH 7.4 (PBS, 10X), sodium phosphate dibasic, orthophosphoric acid, and silver wire (0.5 mm diameter, 99.99%) were obtained from Fisher Scientific (Fair Lawn, NJ, U.S.A). HPLC grade acetonitrile and methanol were procured from Pharmco-Aaper (Brookfield, CT, U.S.A). Polyvinyl alcohol (PVA 26-88 EMPROVE® exp Ph Eur, USP, JPE) and carboxymethyl cellulose sodium salt (CMC low viscosity CAS 9004-32-4) were obtained from Sigma Aldrich (St. Louis, MO, U.S.A). Silver chloride electrodes (2 mm x 4mm) were purchased from A-M systems (Sequim, WA, U.S.A.). Staining solutions, namely methylene blue and Fluoresoft (0.35%), were procured from Eastman Kodak Co. (Rochester, NY, U.S.A) and Holles Laboratories Inc. (Cohasset, MA, U.S.A), respectively. Full-thickness human skin tissue was procured from the National Disease Research Interchange (NDRI) with support from NIH grant U42OD11158.

Dandekar A.A., Garimella H.T., German C.L, & Banga A.K. (2022). Microneedle Mediated Iontophoretic Delivery of Tofacitinib Citrate. Pharmaceutical Research, 40(3), 735-747.

+ Open protocol

+ Expand

Comprehensive Fentanyl Analyte Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The primary reference materials for fentanyl, norfentanyl, acetyl fentanyl, acetyl norfentanyl, and their respective internal standards (ISTD) fentanyl-d₅, norfentanyl-d₅, acetyl fentanyl-¹³C₆, and acetyl norfentanyl-¹³C₆ were purchased from Cerilliant (Round Rock, Texas). Ammonium formate, ammonium hydroxide, glacial acetic acid, isopropanol, methylene chloride, methanol, sodium phosphate monobasic, sodium phosphate dibasic and deionized (DI) water were purchased from Fisher Scientific (Hanover Park, Illinois). All reagents were ACS grade or better. SPEC® MP3 solid phase extraction (SPE) columns were purchased from Agilent Technologies, Inc, (Santa Clara, California). Medical grade nitrogen was purchased from National Welders Supply Company (Richmond, Virginia). Drug-free expired whole blood was obtained from Transfusion Medicine Laboratory at Virginia Commonwealth University Health System. The blood was certified drug free by screening for alkaline extractable drugs by GC/MS and UPLC/MS/MS prior to use.

Poklis J., Poklis A., Wolf C., Mainland M., Hair L., Devers K., Chrostowski L., Arbefeville E., Merves M, & Pearson J. (2015). Postmortem Tissue Distribution of Acetyl Fentanyl, Fentanyl and their Respective Nor-Metabolites Analyzed by Ultrahigh Performance Liquid Chromatography with Tandem Mass Spectrometry. Forensic science international, 257, 435-441.

+ Open protocol

+ Expand

Comprehensive Analytical Reagent Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

Synthesis of Inorganic Nanoparticles

Check if the same lab product or an alternative is used in the 5 most similar protocols

Dopamine hydrochloride (98%), iron(III) chloride hexahydrate (>99%), iron(II) chloride tetrahydrate (>99%) sodium chloride (>99%) and citric acid (>99.5%) were purchased from Sigma-Aldrich (St. Louis, MO, USA) and used as received. Sodium bicarbonate (>99%), sodium carbonate (>99%), tris(hydroxymethyl) aminomethane (Tris), sodium phosphate dibasic (99.2%) and magnesium chloride hexahydrate (>99%) were purchased from Fisher Scientific (Hampton, NH, USA) and used as received. Calcium chloride dihydrate (>99%) was purchased from BDH VWR International (Radnor, PA, USA). Water was purified (18.2 MΩ·cm) using Direct-Q 3 UV-R system (EMD Millipore, Billerica, MA, USA). Silicon wafers with native oxide were purchased from Silicon Quest International (San Jose, CA, USA; 1” diameter, phosphorus doped). Indium tin oxide (ITO) pieces were purchased from University Wafer (Boston, MA, USA; 20 Ω/sq; ITO on glass).

Klosterman L, & Bettinger C.J. (2016). Calcium-Mediated Control of Polydopamine Film Oxidation and Iron Chelation. International Journal of Molecular Sciences, 18(1), 14.

+ Open protocol

+ Expand

Analytical Reagent Preparation and Handling

Check if the same lab product or an alternative is used in the 5 most similar protocols

ACS or HPLC grade acetone, chloroform, methanol, trifluoroacetic acid (TFA), ammonium hydroxide (NH₄OH), acetonitrile, potassium hydroxide (KOH), and sodium phosphate dibasic (Na₂HPO₄) were purchased from Fisher Scientific (Fair Lawn, NJ, USA). ACS grade formic acid was purchased from Honeywell (Morris Plains, NJ, USA).

Zhou Y., Gao Y.G, & Giusti M.M. (2020). Accumulation of Anthocyanins and Other Phytochemicals in American Elderberry Cultivars during Fruit Ripening and its Impact on Color Expression. Plants, 9(12), 1721.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!