Aztec software version 3

CuSO₄·5H₂O and Cu(NO₃)₂·3H₂O used for treating plants were purchased from Merck (Germany). The ultrapure water for watering plants was obtained with a Merck Millipore Direct-Q UV3 system (Darmstadt, Germany).
Acetone used for assimilating pigments extraction from wheat plants was obtained from Chimopar, Romania, Folin-Ciocalteu reagent, gallic acid, anhydrous carbonate, 2,2′-Diphenyl-picrylhydrazyl (DPPH) and 6-hydroxy-2,5,7,8-tetramethylchroman-2 carboxylic acid (Trolox) used for total phenolic content quantification and antioxidant capacity determination were sourced from Sigma-Aldrich, Germany, and the ethanol used for obtaining extracts was purchased from Chimopar, Romania.
The seeds of wheat were from the Agricultural Research and Development Station at Turda-Cluj.
The ultrathin sections obtained were contrasted with lead citrate and uranyl acetate and examined with a transmission electron microscope (TEM) Jeol JEM 1010 (JEOL, Tokyo, Japan). The samples were analyzed with TEM Hitachi HD2700 (Hitachi, Tokyo, Japan) cold field emission, operated at 200 kV and coupled with an EDX detector (Oxford Instruments, Oxford, UK, AZtec Software, version 3.3) used for elemental detection. All reagents used for this part of the experiment were acquired from Sigma Aldrich (Merk, Bucharest, Romania).

A FEI Helios Xe plasma-FIB was used to prepare a cross-section of the micropillar after the cross-sectional slab was extracted. A cleaning cross-section was employed in the FIB at an accelerating voltage of 30 kV and a current of 59 nA. Subsequently, EBSD mapping was undertaken of the prepared cross-section using an Oxford Instruments NordlysMax detector, employing the SEM at an accelerating voltage of 20 kV and a beam current of 11 nA. Acquisition and data analysis were performed using Oxford Instruments Aztec software (version 3.3).