Igr37

Manufactured by Leibniz Institute DSMZ

Sourced in Germany

IGR37 is a piece of laboratory equipment used for incubating and growing microorganisms. It provides a controlled environment for culturing various types of microbes, such as bacteria, fungi, and algae. The core function of IGR37 is to maintain the optimal temperature, humidity, and other environmental conditions required for the growth and proliferation of the target microorganisms.

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Lab products found in correlation

Ipc298, by Leibniz Institute DSMZ (2 mentions) Wm266 4, by American Type Culture Collection (2 mentions) Wm115, by American Type Culture Collection (2 mentions) Igr39, by Leibniz Institute DSMZ (2 mentions) Fetal bovine serum (fbs), by Harvard Bioscience (1 mentions)

3 protocols using igr37

Sourcing and Culturing Human Melanoma Cell Lines

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Human melanoma cell lines such as IGR37, IGR39, and IPC‐298 were purchased from DSMZ; MEWO cell line was purchased from ICLC; SK‐MEL‐5 and SK‐MEL‐28 were purchased from NCI‐60; WM266.4 was purchased from ATCC; WM115, A‐375 and C32 were purchased from IZSBS; and HEK293T cells were purchased from ICLC.
All the cells were cultured in Dulbecco modified Eagle's medium (DMEM)+ 10% FBS S.A.+ 2 mM l‐Glutamine except for IPC‐298 that was cultured in RPMI‐1640+ 10% FBS S.A.+ 2 mM l‐Glutamine. Cell lines were tested for mycoplasma by mycoplasma PCR Test Kit.

Matafora V., Farris F., Restuccia U., Tamburri S., Martano G., Bernardelli C., Sofia A., Pisati F., Casagrande F., Lazzari L., Marsoni S., Bonoldi E, & Bachi A. (2020). Amyloid aggregates accumulate in melanoma metastasis modulating YAP activity. EMBO Reports, 21(9), e50446.

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Stimulation of Melanoma Cell Lines

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Human malign melanoma cell lines IGR1 and IGR37 were obtained from DSMZ (Braunschweig, Germany). All cells were cultured in 85% Dulbecco's MEM supplemented with 15% h.i. FBS (Biochrom, Berlin, Germany). The cell lines were authenticated by the STR profiling. All the cell cultures were tested for Mycoplasma contamination by PCR before use. For stimulation, the cells were incubated for 24 h, with 5‐fluoro‐2‐deoxyuridine (FdUrd, 10 μmol/L) or with methotrexate (MTX, 10 μmol/L).

Miran T., Vogg A.T., El Moussaoui L., Kaiser H., Drude N., von Felbert V., Mottaghy F.M, & Morgenroth A. (2017). Dual addressing of thymidine synthesis pathways for effective targeting of proliferating melanoma. Cancer Medicine, 6(7), 1639-1651.

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Sourcing and Culturing Melanoma Cell Lines

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Human melanoma cell lines such as IGR37, IGR39 and IPC-298 were purchased from DSMZ;
MEWO cell line was purchased from ICLC; SK-MEL-5 and SK-MEL-28 were purchased from NCI-60; WM266.4 was purchased from ATCC; WM115, A-375 and C32 were purchased from IZSBS.
All the cells were cultured in Dulbecco Modified Eagle's Medium DMEM+ 10% FBS S.A.+ 2 mM L-Glutamine except for IPC-298 that was cultured in RPMI-1640+ 10% FBS S.A.+ 2 mM L-Glutamine. Cell lines were tested for mycoplasma by mycoplasma PCR Test Kit.

Matafora V., Farris F., Restuccia U., Tamburri S., Martano G., Bernardelli C., Pisati F., Casagrande F., Lazzari L., Marsoni S., Bonoldi E., & Bachi A. (2020). Amyloid aggregates accumulate in melanoma metastasis driving YAP mediated tumor progression.

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