Annexin 5 staining kit

Manufactured by Roche

The Annexin V Staining Kit is a laboratory reagent used for the detection and quantification of apoptosis, a form of programmed cell death. The kit contains Annexin V, a protein that binds to phosphatidylserine, a molecule that is exposed on the surface of cells undergoing apoptosis. The kit also includes a counterstain, such as propidium iodide, to differentiate between apoptotic and necrotic cells.

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Lab products found in correlation

Anti γh2ax 05 636, by Merck Group (1 mentions) Ab36823, by Abcam (1 mentions) Ab22551, by Abcam (1 mentions) Ab206386, by Abcam (1 mentions) Mab3868, by Merck Group (1 mentions)

2 protocols using annexin 5 staining kit

Detecting DNA Damage and Apoptosis

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γH2AX and 53BP1 immunostaining was carried out as previously described(Mao et al, 2011 (link)). Anti‐ γH2AX (05–636) and anti‐53BP1 (MAB3804) antibodies were purchased from Millipore. Apoptosis in fibroblasts was measured using the Annexin V Staining Kit (Roche).

Simon M., Yang J., Gigas J., Earley E.J., Hillpot E., Zhang L., Zagorulya M., Tombline G., Gilbert M., Yuen S.L., Pope A., Van Meter M., Emmrich S., Firsanov D., Athreya A., Biashad S.A., Han J., Ryu S., Tare A., Zhu Y., Hudgins A., Atzmon G., Barzilai N., Wolfe A., Moody K., Garcia B.A., Thomas D.D., Robbins P.D., Vijg J., Seluanov A., Suh Y, & Gorbunova V. (2022). A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A. The EMBO Journal, 41(21), e110393.

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DNA Damage and Apoptosis Assays

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γH2AX and 53PB immunostaining was carried out as previously described (Mao et al., 2011 (link)). Anti-γH2AX and anti-53BP1 antibody was purchased from Abcam (ab22551 and ab36823). For ssDNA immunostaining, cells were fixed with PFA, followed by 24hr methanol incubation at −20°C. Cells were then incubated at 37°C for 4hrs with RNaseA. Antibody from Millipore (MAB3868). Apoptosis in fibroblasts was measured using the Annexin V Staining Kit (Roche). Apoptosis in tissues was measured using TUNEL method with in situ apoptosis detection kit, Abcam (ab206386).

Simon M., Meter M.V., Ablaeva J., Ke Z., Gonzalez R.S., Taguchi T., Cecco M.D., Leonova K.I., Kogan V., Helfand S.L., Neretti N., Roichman A., Cohen H.Y., Meer M.V., Gladyshev V.N., Antoch M.P., Gudkov A.V., Sedivy J.M., Seluanov A, & Gorbunova V. (2019). LINE1 derepression in aged wild type and SIRT6 deficient mice drives inflammation. Cell metabolism, 29(4), 871-885.e5.

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