Aviii hd 500 nmr spectrometer

On the second harvest date, on 24th September 2021, metabolite and nutrient contents in the lime juice of the fruits sampled for quality analysis were assessed. Juice samples were centrifuged at 10,000 rpm for 30 min at a temperature of 4 °C, and the supernatant was filtered through a 0.45 µm chromatography filter. The content of primary metabolites (sugar, amino acids, and organic acids) and nutrients in these juice samples were then analyzed. The concentration of primary metabolites was quantified by H NMR spectra, recorded at 298 K on a Bruker AVIII HD 500 NMR spectrometer (500.13 MHz for 1H) equipped with a 5 mm CPP BBO cryogenic probe (Bruker Biospin, Ettlingen, Germany). Analysis of metabolites was assessed by the Metabolomics Platform of CEBAS-CSIC (http://www.cebas.csic.es/general_english/metabolomics.html (accessed on 24 September 2021)), while mineral content was assessed by the Ionomics Service of CEBAS-CSIC (http://www.cebas.csic.es/general_english/ionomics.html (accessed on 24 September 2021)), using Inductively Coupled Plasma (ICP-ICAP 6500 DUO Thermo, Horsham, England).

About 5–10 mg of the fractions obtained from CC purification was dissolved in CDCl₃, DMSO-d₆, or acetone-d₆ and spectra were acquired using a Bruker AVIII-HD-500 NMR spectrometer.