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Sigma a5228

Manufactured by Merck Group
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Sigma A5228 is a laboratory equipment product manufactured by Merck Group. It is a general-purpose device designed to perform essential laboratory functions. The core function of this product is to assist in the execution of scientific experiments and research activities.

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Lab products found in correlation

Ab6584, by Abcam (1 mentions) Nb600 408, by Abcam (1 mentions) Ab110128, by Abcam (1 mentions)

2 protocols using sigma a5228

1

Immunohistochemistry of Cytoskeletal Proteins

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Sections were stained for αSMA (1:200; Sigma A5228), collagen type I (1:200; Novus NB600‐408) fibronectin (1:500; Abcam ab6584), and calponin (1:200; Abcam ab110128). All samples were blocked with 5% normal donkey serum for 2 h before incubation in the primary antibody overnight. The samples were then stained with a Cy3 fluorescent‐labelled secondary antibody (Jackson Immuno Lab) at 1:200 dilution and nuclear Hoechst stain (1:10 000).
Weidenhamer N.K., Moore D.L., Lobo F.L., Klair N.T, & Tranquillo R.T. (2015). Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. Journal of Tissue Engineering and Regenerative Medicine, 9(5), 605-618.
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2

Quantifying αSMA Expression in hMSC Matrices

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Sections were taken from mottled areas of hMSC‐seeded matrices treated with ascorbic acid for 1 week. Tissues were stained for αSMA (1:200; Sigma A5228) and Cy5 fluorescent‐labelled secondary antibody (1:400; Jackson Immuno lab) for imaging. The average pixel intensity was measured with ImageJ for each image located within the translucent or opaque regions. Values from multiple images within sections were averaged and used to compare relative αSMA expression between the two regions.
Weidenhamer N.K., Moore D.L., Lobo F.L., Klair N.T, & Tranquillo R.T. (2015). Influence of culture conditions and extracellular matrix alignment on human mesenchymal stem cells invasion into decellularized engineered tissues. Journal of Tissue Engineering and Regenerative Medicine, 9(5), 605-618.
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