Aav5 syn flex chrimsonr tdt

Manufactured by Addgene

AAV5-Syn-FLEX-ChrimsonR-tdT is a recombinant adeno-associated virus (AAV) vector that expresses the optogenetic tool ChrimsonR and a red fluorescent reporter tdTomato (tdT) under the control of the Synapsin (Syn) promoter. The FLEX switch allows for Cre-dependent expression.

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Aavrg cag gfp, by Addgene (1 mentions) Aavrg cag flex tdtomato, by Addgene (1 mentions) Aav5 hsyn dio hm4d gi mcherry, by Addgene (1 mentions) Aav5 hsyn dio hm3d gq mcherry, by Addgene (1 mentions)

2 protocols using aav5 syn flex chrimsonr tdt

Versatile AAV Toolbox for Optogenetics

Cited 2 times

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All adeno-associated viruses (AAVs) were purchased from Addgene or custom-synthesized by the University of Pennsylvania vector core. The following AAVs were used: AAVrg-CAG-FLEX-tdTomato (Addgene 59462-AAVrg), AAVrg-CAG-GFP (Addgene: 37825-AAVrg), AAV5-pCAG-FLEX-EGFP (Addgene: 51502-AAV5), AAV9-EF1a-dfloxed-hChR2-EYFP (Addgene: 20298-AAV9), AAV5-Syn-FLEX-ChrimsonR-tdT (Addgene: 62723-AAV5), AAV9-EF1a-DIO-HA-SNAP-mGluR2-WPRE was previously reported.^{45 (link),48 (link)} PORTL compound BGAG_12,400 was synthesized by J. Broichhagen as previously described. ^{48 (link)}

Munguba H., Gutzeit V.A., Srivastava I., Kristt M., Singh A., Vijay A., Arefin A., Thukral S., Broichhagen J., Stujenske J.M., Liston C, & Levitz J. (2024). Projection-Targeted Photopharmacology Reveals Distinct Anxiolytic Roles for Presynaptic mGluR2 in Prefrontal- and Insula-Amygdala Synapses. bioRxiv.

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AAV Vectors for Neuroscience Research

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Viruses used in this study were either acquired commercially or produced locally. The following AAV plasmids were used and detailed sequence information is available as detailed or upon request: AAVDj-hSyn-flex-mGFP-2A-synaptophysin-mRuby^{57 (link)} (Stanford Vector Core Facility, reference AAV DJ GVVC-AAV-100), AAV5-CAG-DIO-COMET-tdTomato and AAV5CAG-COMET-GFP (a gift from M. Tuszynski), AAV5-Syn-flex-ChrimsonR-tdT (Addgene 62723), AAV5-CAG-flex-Jaws-KGC-GFP-ER2 (Addgene 84445), AAV5-hSyn-DIO-hm4D (Gi)-mCherry (Addgene 44362), AAV5-hSyn-DIO-hm3D (Gq)-mCherry (Addgene 44361), AAV5-CAG-flex-tdTomato (a gift from S. Arber), AAV5CAG-flex-human diphtheria toxin receptor (DTR) (a gift from S. Arber), AAV5-DIO-TC66T-2A-eGFP-2A-oG (GT3) (Salk Institute) and AAV5-hSyn-DIO-TVAP2A-EGFP-2A-oG (a gift from T. Karayannis). All floxed AAV vectors used in the present study showed transgene expression only upon Cre-mediated recombination. Trans-synaptic tracings were performed with EnvA-G-deleted rabies–mCherry (GT3) (Salk Institute 32646) or the cre-dependent PRV Ba2017 (expressing GFP; 4.9 × 10⁹ pfu per ml; Princeton University). Injection volumes, coordinates and experimental purpose are described below.

Kathe C., Skinnider M.A., Hutson T.H., Regazzi N., Gautier M., Demesmaeker R., Komi S., Ceto S., James N.D., Cho N., Baud L., Galan K., Matson K.J., Rowald A., Kim K., Wang R., Minassian K., Prior J.O., Asboth L., Barraud Q., Lacour S.P., Levine A.J., Wagner F., Bloch J., Squair J.W, & Courtine G. (2022). The neurons that restore walking after paralysis. Nature, 611(7936), 540-547.

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