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4 hydroxybenzyl gls

Manufactured by Carl Roth
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Sourced in Germany

4-Hydroxybenzyl GLS is a chemical compound that can be used as a laboratory reagent. It serves as a substrate for various enzymatic reactions and analytical procedures. The product specifications and technical details are available upon request.

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Lab products found in correlation

Benzonitrile, by Merck Group (4 mentions) 3 butenenitrile, by Merck Group (3 mentions) 4 pentenenitrile, by Merck Group (3 mentions) 3 phenylpropanenitrile, by Merck Group (3 mentions) Methylene chloride gc ultra grade, by Carl Roth (2 mentions) 2 phenylethyl isothiocyanate, by Merck Group (1 mentions) 2 propenyl itc, by Merck Group (1 mentions) 3 methylthio propyl itc, by Merck Group (1 mentions) Myrosinase, by Merck Group (1 mentions) Cacl 2 h 2 o 2, by Merck Group (1 mentions) Feso4 h2o 7, by Merck Group (1 mentions) Mgcl2 h2o 6, by Merck Group (1 mentions) Nahco3, by Merck Group (1 mentions) Kh2po4, by Merck Group (1 mentions) L ascorbic acid, by Merck Group (1 mentions) Acetic acid, by Merck Group (1 mentions) Potassium chloride (kcl), by Merck Group (1 mentions) Dl dithiothreitol, by Merck Group (1 mentions) Phenol chloroform isoamyl alcohol, by Carl Roth (1 mentions) Chloroform isoamylalcohol, by Carl Roth (1 mentions)

4 protocols using 4 hydroxybenzyl gls

1

Purchasing Standard Compounds for Research

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Benzonitrile (≥99.9%), 3-butenenitrile (2Prop-CN; ≥98%), 3-(methylthio)propyl ITC (3MTP-ITC; ≥98%), 2-propenyl ITC (2Prop-ITC; ≥99%), 4-pentenenitrile (3But-CN; ≥97%), 3-phenylpropanenitrile (2PE-CN; ≥99%), and 2-phenylethyl isothiocyanate (2PE-ITC; ≥99%) were purchased from Sigma–Aldrich Chemie GmbH (Steinheim, Germany). 1-Cyano-2,3-epithiopropane [CETP; ≥97.6% (by GC-MS)] was purchased from Taros Chemicals GmbH Co. KG (Dortmund, Germany). 3-Indoleacetonitrile (IAN) (≥98%) was acquired from Acros Organics (Fischer Scientific GmbH, Schwerte, Germany). 3-Butenyl ITC (3But-ITC; ≥95%) was obtained from TCI Deutschland GmbH (Eschborn, Germany). 3-(Methylsulfinyl)propyl ITC (3MSOP-ITC) and 4-(methylthio)butyl ITC (4MTB-ITC; ≥98%) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany). 4-(Methylsulfinyl)butyl ITC (4MSOB-ITC) was bought from Enzo Life Sciences GmbH (Lörrach, Germany). (R)-5-Vinyloxazolidine-2-thione (R-OZT) was purchased from Biosynth AG (Staad, Switzerland). 4-Hydroxybenzyl GLS (≥99%) and methylene chloride (GC Ultra grade) was obtained from Carl Roth GmbH (Karlsruhe, Germany). Acetonitrile (Ultra Gradient HPLC grade) was procured from J.T. Baker (Deventer, The Netherlands). NaSO4 (≥99%) and methanol (>99.9) was purchased from VWR International GmbH (Darmstadt, Germany).
All solvents were of LC-MS or GC-MS grade, water was of Milli-Q quality.
Hanschen F.S, & Schreiner M. (2017). Isothiocyanates, Nitriles, and Epithionitriles from Glucosinolates Are Affected by Genotype and Developmental Stage in Brassica oleracea Varieties. Frontiers in Plant Science, 8, 1095.
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2

Preparation of Simulated Salivary Fluid

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Acetic acid (≥99.5%), 2Prop-GLS (≥99%), l-ascorbic acid (≥99%), benzonitrile (≥99.9%), d/l-dithiothreitol, FeSO4(H2O)7 (≥99%), myrosinase (thioglucosidase from Sinapis alba seeds; ≥100 units/g) and NaHCO3 (≥99.5%) were purchased from Sigma-Aldrich Chemie GmbH, (Steinheim, Germany). 4-Hydroxybenzyl GLS (≥99%) was purchased from Carl Roth GmbH, Karlsruhe, Germany and NaCH3COO(H2O)3 (≥99.5%) was purchased from Merck (Darmstadt, Germany), respectively. Simulated salivary fluid (saliva buffer) was prepared according to60 (link) (pH 7) using chemicals from Merck (Darmstadt, Germany) (KCl, KH2PO4, MgCl2(H2O)6, CaCl2(H2O)2) and Sigma-Aldrich Chemie GmbH, (Steinheim, Germany) (α-amylase. NaHCO3, HCl (37%), (NH4)2CO3 (≥30%, NH3 basis)) with a purity of ≥99% if not stated otherwise. All solvents were of LC-MS grade and water was of Milli-Q quality.
Hanschen F.S., Klopsch R., Oliviero T., Schreiner M., Verkerk R, & Dekker M. (2017). Optimizing isothiocyanate formation during enzymatic glucosinolate breakdown by adjusting pH value, temperature and dilution in Brassica vegetables and Arabidopsis thaliana. Scientific Reports, 7, 40807.
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3

Comprehensive Phytochemical Compound Database

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2Prop-ITC, (≥99%), benzonitrile (≥99.9%), 3-butenenitrile (2Prop-CN, ≥98%), 4-pentenenitrile (3But-CN, ≥97%), 3-phenylpropanenitrile (2PE-CN, ≥99%) and sucrose-sodium nitrate media were purchased from Sigma-Aldrich Chemie GmbH, Steinheim, Germany; IAN, (≥98%) from Acros Organics (Fischer Scientific GmbH, Schwerte, Germany); 3-butenyl ITC (3But-ITC, ≥95%) and 4-pentenyl ITC (4Pent-ITC, ≥95%) were purchased from TCI Deutschland GmbH, Eschborn, Germany; 3-hydroxypropionitrile was purchased from Thermo Fischer Scientific, Erembodegem, Belgium; 4-(methylthio)butyl ITC (4MTB-ITC, ≥98%) was purchased from Santa Cruz Biotechnology, Heidelberg, Germany; 5-(methylsulfinyl)pentyl ITC (5MSOP-ITC) was purchased from Enzo Life Sciences GmbH, Lörrach, Germany; 1-cyano-2,3-epithiopropane (CETP), (≥95%) was purchased from Taros Chemicals GmbH Co. KG, Dortmund, Germany; 4-hydroxybenzyl GLS (≥97%), methylene chloride (GC Ultra Grade), Tris, EDTA, NaCl, CTAB, chloroform/isoamylalcohol (24:1), β-mercaptoethanol and phenol/chloroform/isoamylalcohol (25:24:1) from Carl Roth GmbH, Karlsruhe, Germany; acetonitrile (Ultra Gradient HPLC grade) was purchased from J.T. Baker, Deventer, The Netherlands and NaSO4 (≥99%) and methanol (>99.9) were purchased from VWR International GmbH, Darmstadt, Germany. Potato dextrose agar was purchased from Merck, Darmstadt, Germany. All solvents were of LC or GC-MS grade.
Witzel K., Hanschen F.S., Klopsch R., Ruppel S., Schreiner M, & Grosch R. (2015). Verticillium longisporum infection induces organ-specific glucosinolate degradation in Arabidopsis thaliana. Frontiers in Plant Science, 6, 508.
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4

Synthesis and Characterization of Glucosinolates

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4-hydroxybenzyl GLS (≥ 99%), methylene chloride (GC Ultra Grade) and 2-propenyl GLS were purchased from Carl Roth GmbH + Co. KG (Karlsruhe, Germany); allyl ITC (≥ 99%), aryl sulfatase, benzonitrile (≥ 99.9%), 3-butenenitrile (≥ 98%), DEAE-Sephadex A-25, 4pentenenitrile (≥ 97%), and 3-phenylpropanenitrile (≥ 99%) were obtained from Sigma-Aldrich Chemie GmbH (Steinheim, Germany); 3-butenyl ITC (≥ 95 %) and 4-pentenyl ITC (≥ 95%) were purchased from TCI Deutschland GmbH (Eschborn, Germany); NaSO4 anhydrous (≥ 99%) was obtained from VWR International GmbH (Darmstadt, Germany); NaHCO3 (p.A, ≥ 99%) was purchased from Merck (Darmstadt, Germany); 3-(methylsulfinyl)propyl ITC and 4-(methylsulfanyl)butyl ITC (≥ 98 %) were purchased from Santa Cruz Biotechnology (Heidelberg, Germany); 4MSOB-ITC was purchased from Enzo Life Sciences GmbH (Lörrach, Germany). The ETN 1-cyano-2,3-epithiopropane (≥ 95 %) was synthetized by Taros Chemicals GmbH Co. KG (Dortmund, Germany) and 1-cyano-3,4-epithiobutane was synthetized by ASCA GmbH Angewandte Synthesechemie Adlershof (Berlin, Germany). 5-(Methylsulfanyl)pentanenitrile and 5-(methylsulfinylpentanenitrile were purchased from Enamine (SIA Enamine, Latvia, Riga). Methanol (≥ 99.95 %), acetonitrile (LC-MS grade), and arylsulfatase were purchased from Th. Geyer GmbH & Co. KG (Renningen, Germany) and ultrapure water was used.
Hanschen F.S. (2020). Domestic boiling and salad preparation habits affect glucosinolate degradation in red cabbage (Brassica oleracea var. capitata f. rubra). Food chemistry, 321.
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