Anti myc

Manufactured by CWBIO

Sourced in China

The Anti-Myc is a laboratory equipment product designed for the detection and analysis of c-Myc, a cellular transcription factor. It is a high-affinity monoclonal antibody that specifically recognizes the c-Myc protein and can be used in various applications such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Anti ha, by CWBIO (2 mentions) Anti gapdh, by CWBIO (1 mentions) Anti gfp, by Transgene (1 mentions) Gfp agarose beads, by Proteintech (1 mentions) Anti gfp, by CWBIO (1 mentions) Anti ha, by Merck Group (1 mentions)

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Anti-c-Myc (2 citations)

3 protocols using anti myc

Reconstitution of NAIP-NLRC4 inflammasome

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Full-length NAIP5 and NAIP2 genes were obtained by DNA synthesis. Mouse NLRC4, pro IL-1β and pro caspase-1 were amplified from reverse-transcribed mouse cDNAs. Full-length PrgJ and FliC genes were amplified from S. typhimurium genomic DNA. NLRC4-R288A-L435D-1 008-1 012^DDYD−AAAA(NLRC4^M), all NAIP5 and flagellin mutations were generated by standard molecular biology procedures. FliC_D0_L was designed as described before^{32 (link)}. All constructs were verified by sequencing.
Antibody used: anti-Myc (cw0299, CWBIO), anti-HA (cw0092, CWBIO), anti-IL-1β (GTX74034, GeneTex) and anti-GAPDH (cw0100, CWBIO).

Yang X., Yang F., Wang W., Lin G., Hu Z., Han Z., Qi Y., Zhang L., Wang J., Sui S.F, & Chai J. (2017). Structural basis for specific flagellin recognition by the NLR protein NAIP5. Cell Research, 28(1), 35-47.

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In vitro Ubiquitination Assay Protocol

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The in vitro ubiquitination assays were performed as described previously [66 (link)]. In brief, A 100 ng quantity of wheat (Triticum aestivum) E1, 200 ng of purified E2, 5 mg of Myc-tagged ubiquitin (Boston Biochemicals, Cambridge, MA, USA), 1 mg of purified GST-SRAS1.1, GST-SRAS1.2 and CSN5A-His or CSN5B-His were added to 30 μl of ubiquitination reaction buffer (50 mM Tris-HCl pH 7.5, 2 mM ATP, 5 mM MgCl₂, 2 mM DTT). After 24 h at 30°C the reactions were stopped by adding 5×loading buffer, the samples were then boiled at 100°C for 5 min. The products were electrophoresed on a 15% SDS polyacrylamide gel electrophoresis (PAGE) gel and detected with anti-His (CWBIO, Beijing, China) and anti-Myc (CWBIO, Beijing, China) antibodies by western blotting.

Zhou Y., Li X.H., Guo Q.H., Liu P., Li Y., Wu C.A., Yang G.D., Huang J.G., Zhang S.Z., Zheng C.C, & Yan K. (2021). Salt responsive alternative splicing of a RING finger E3 ligase modulates the salt stress tolerance by fine-tuning the balance of COP9 signalosome subunit 5A. PLoS Genetics, 17(11), e1009898.

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Investigating SIZ1-HLS1 and HLS1-phyB Interactions

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For SIZ1-HLS1 interaction, 3-d-old dark-grown HLS1pro:HA-FLAG-HLS1 SIZ1pro:SIZ1-GFP and HLS1pro:HA-FLAG-HLS1 SIZ1pro:GFP transgenic seedlings were used for Co-IP assays (Zhang et al. 2021) . The total proteins were extracted from different seedlings and then incubated with GFP agarose beads (Chromotek) in IP buffer [10 mM Tris-HCl, pH 7.5, 0.5% (v/v) Nonidet P-40, 2 mM EDTA, 150 mM NaCl, 1 mM PMSF, and 1% (w/v) protease inhibitor]. The beads were collected and washed at least 5 times with IP buffer, then the interaction by immunoblotting was examined using anti-HA (1:5,000 dilution, Sigma-Aldrich, USA, H6908) and anti-GFP (1:5,000 dilution, Transgen, China, HT801) antibodies. For HLS1-phyB interaction, HA-HLS1, MYC-SUMO1, GFP, and phyB-GFP plasmids were transformed into Arabidopsis protoplasts isolated from Col-0 or siz1-2. Arabidopsis protoplasts were prepared as described previously (Yoo et al. 2007 (link)). After 16 h of expression in the darkness, protoplasts were transferred to light for 2 h, and then proteins were extracted and incubated with HA agarose beads in IP buffer. The anti-HA, anti-MYC (1:5,000 dilution, Cwbio, China, cw0299M), and anti-GFP antibodies were used for immunoblotting. The antibody source details are listed in Supplemental Table 1.

Xiong J., Yang F., Wei F., Yang F., Lin H, & Zhang D. (2023). Inhibition of SIZ1-mediated SUMOylation of HOOKLESS1 promotes light-induced apical hook opening in Arabidopsis. The Plant cell, 35(6).

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