Buprivacaine

For ICV infusions of SCDi or vehicle, mice were locally injected with buprivacaine (Hospira) and operated under isoflurane anesthesia (Baxter). Brain cannulae attached to Alzet osmotic pumps were stereotaxically implanted at 0.0 mm antero-posterior and 0.9 mm lateral to Bregma and the pumps placed under the back skin according to manufacturer's instructions. The 28-day Alzet osmotic pumps used in these experiments (0.11 μl/h infusion rate, model 1004; Durect) were primed for 48 h and began pumping immediately when implanted. The SCD inhibitor was dissolved in DMSO (Sigma-Aldrich) and infused at a final concentration of 80 µM in sterile aCSF (148 mM NaCl, 3 mM KCl, 1.7 mM MgCl₂, 1.4 mM CaCl₂, 1.5 mM Na₂PO4, 0.1 mM NaH₂PO₄). Vehicle pumps contained the same volumes as the SCDi pumps (0.8% DMSO/aCSF).

For ICV infusions of SCDi or vehicle, mice were locally injected with buprivacaine (Hospira) and operated under isoflurane anesthesia (Baxter). Brain cannulae attached to Alzet osmotic pumps were stereotaxically implanted at 0.0 mm antero-posterior and 0.9 mm lateral to Bregma and the pumps placed under the back skin according to manufacturer’s instructions. The 28-day Alzet osmotic pumps used in these experiments (0.11 μl/h infusion rate, model 1004; Durect) were primed for 48 h and begin pumping immediately when implanted. Abcam or Cayman SCD inhibitors were dissolved in DMSO (Sigma-Aldrich) and infused at a final concentration of 80 µM in sterile aCSF (148 mM NaCl, 3 mM KCl, 1.7 mM MgCl₂, 1.4 mM CaCl₂, 1.5 mM Na₂PO₄, 0.1 mM NaH₂PO₄). Vehicle pumps contained the same volumes as the SCDi pumps (0.8% DMSO/aCSF).