Spectrum two infrared

Manufactured by PerkinElmer

Sourced in United States

The Spectrum Two infrared is a laboratory instrument designed for spectroscopic analysis. It is capable of detecting and identifying chemical compounds by their unique infrared absorption spectrum.

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Lab products found in correlation

Dionex ultimate 3000, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Spectrum Two Fourier transform infrared (FTIR) spectrophotometer (2 citations) Spectrum TWO infrared spectrophotometer (2 citations) Spectrum Two) Fourier transform infrared spectrophotometer (2 citations) Spectrum Two Fourier-transform infrared spectrometer (3 citations) Spectrum Two Infrared Spectrometer (5 citations) Spectrum Two Fourier transform infrared (FTIR) spectrometer (3 citations) Spectrum two Fourier transform infrared spectroscopy (2 citations) Spectrum Two (426 citations)

5 protocols using spectrum two infrared

Analytical Characterization of Organic Compounds

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Nuclear magnetic resonance (NMR) spectra were obtained using a 300 MHz Bruker Fourier spectrometer and a 400 MHz Bruker Fourier spectrometer (Bruker, Mannheim, Germany). Melting point determinations were performed on a Stuart Scientific SMP10 (Merck KGaA, Darmstadt, Germany) model with 230 V AC/DC capability. Optical rotation measurements were carried out in chloroform solutions utilizing an Anton Paar MC100 polarimeter (Anton Paar, Graz, Austria). Attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectra were recorded on a PerkinElmer Spectrum Two infrared spectrophotometer equipped with a Universal ATR. Liquid chromatography-high resolution mass spectrometry (LC-HRMS) data were acquired using a Dionex Ultimate 3000 ultra high-performance liquid chromatograph (UHPLC) system (Thermo Fisher Scientific, Waltham, MA, USA) coupled to a Thermo Scientific Q Exactive hybrid quadrupole-Orbitrap mass analyzer. The UHPLC component consisted of a multiple wavelength detector and an imChem Surf C18 TriF reverse phase column, applying a 10 min linear solvent gradient of 20–30% aqueous acetonitrile at a 0.2 mL/min flow rate alongside 250 nm UV detection. All solvents were distilled from commercial-grade sources. Pyridine and benzoyl/anhydride reagents were used without previous purification.

Isca V.M., Sitarek P., Merecz-Sadowska A., Małecka M., Owczarek M., Wieczfińska J., Zajdel R., Nowak P., Rijo P, & Kowalczyk T. (2024). Anticancer Effects of Abietane Diterpene 7α-Acetoxy-6β-hydroxyroyleanone from Plectranthus grandidentatus and Its Semi-Synthetic Analogs: An In Silico Computational Approach. Molecules, 29(8), 1807.

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FTIR Analysis of Polymerized Materials

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FTIR spectra were acquired from a Perkin Elmer® Spectrum Two infrared spectrometer equipped with a UATR (universal attenuated total reflectance crystal) 2 module. Liquids were placed directly onto and completely covering the crystal sample window. Solid materials of various compositions used were polymerized into 1 mm slabs and then cut into disks with a 6 mm biopsy punch. Spectra from the material-face distal to the UV light source are reported. Spectra from material-faces were shown to be consistent at the 927 cm⁻¹ peak indicating the alkene-associated carbon-hydrogen bond. This alkene-associated carbon-hydrogen bond is affected by alkene-thiol conjugation and indicative of polymerization of the material.

Warner J.J., Wang P., Mellor W.M., Hwang H.H., Park J.H., Pyo S.H, & Chen S. (2019). 3D Printable Non-Isocyanate Polyurethanes with Tunable Material Properties. Polymer chemistry, 10(34), 4665-4674.

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FTIR Analysis of Protein Secondary Structure

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Proper modifications were performed following the method of Tang et al. [17] (link), and FTIR spectra were recorded on a Spectrum Two infrared spectrometer (PerkinElmer, Shelton, CT, USA). The gel freeze-dried powder was dried thoroughly using P 2 O 5 in a dryer and placed directly on the germanium crystal surface of a horizontal ATR instrument. After tablet pressing, the sample plate required for the FTIR experiment was obtained. A total of 32 scans with absorption spectra measured in the range of 4000-400 cm -1 and the background spectrum scanned with a resolution of 4 cm -1 were used. The relative proportions of α-helix, β-sheet, β-turn and random coil percentages were calculated using Peak Fit v4.12 software by selecting the amide I band in the spectral region of 1600-1700 cm -1 .

Xia X., Zhang B., Huang Y., Zhu Y., Qu M., Liu L., Sun B, & Zhu X. (2024). Soy Protein Isolate Gel Subjected to Freezing Treatment: Influence of Methylcellulose and Sodium Hexametaphosphate on Gel Stability, Texture and Structure. Foods (Basel, Switzerland), 13(13).

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Estimating Pectin Degree of Esterification Using FT-IR

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The degree of esterification (DE) of pectin samples was estimated in triplicate by means of Fourier transform infrared spectroscopy (FT-IR) analysis using a Spectrum Two infrared spectrophotometer (Perkin Elmer, USA). The spectra were recorded in transmission mode within the wavenumber range of 4000-400 cm -1 at a resolution of 4 cm -1 . SpectraGryphspectroscopy software (Version 1.2.11) was used to display the spectra.
Since the DE is defined as the number of esterified carboxylic groups over the number of total carboxylic groups multiplied by 100, it is inferred that the ratio of the area of the band at 1730 cm -1 , which corresponds to the number of esterified carboxylic groups, to the sum of the areas of the bands between 1730 and 1600 cm -1 that corresponds to the number of total carboxylic groups, should be proportional to the DE (Wai, AlKarkhi, & Easa, 2010; (link)Manrique & Lajolo, 2002) (link):

Dranca F., Vargas M, & Oroian M. (2020). Physicochemical properties of pectin from Malus domestica ‘Fălticeni’ apple pomace as affected by non-conventional extraction techniques. Food Hydrocolloids., 100.

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Estimating Pectin Degree of Esterification Using FT-IR

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Dranca F., Vargas M., & Oroian M. (2020). Physicochemical properties of pectin from Malus domestica ‘Fălticeni’ apple pomace as affected by non-conventional extraction techniques. Food Hydrocolloids, 100, 105383-105383.

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