D800 digital camera

The animal experiments were approved by the Committee on Animal Care of the First Affiliated Hospital of Nanjing Medical University and were conducted according to NIH Guidelines for the Care and Use of Laboratory Animals. All studies involving animals are reported in accordance with the ARRIVE guidelines for reporting experiments involving animals. Nude mice were purchased from the Vital River Laboratories (Beijing, People’s Republic of China). A total of 6×10⁶ HepG2 cells were transfected with miR-378 mimics or NC. Cells were cultured for 24 h before harvest for transplantation into the animal. Before injection, cells were pooled and mixed with Matrigel (BD Biosciences). A total of 3×10⁶ cells were injected subcutaneously into the left flank of nude mice. Tumor volumes were calculated using the formula V = length × width ^2/2. The animals were sacrificed 4 weeks after injection. Pictures were recorded with a Nikon d800 digital camera (Nikon, Tokyo, Japan).

All animal work was conducted under the institutional guidelines of Shandong Province and approved by the Use Committee for Animal Care (Animal Ethics Committee of Binzhou Central Hospital). Nude mice were purchased from the Vital River Laboratories (Beijing, China). 8×10⁶ HGC-27 cells were transfected with miR-375 inhibitor, siTINCR, or control in replicate. Cells were cultured for 24 h before harvesting for transplantation into the animal. Before injection, cells were pooled and mixed with matrigel (BD Biosciences, San Jose, CA, USA). 3×10⁶ cells were injected subcutaneously into the left flank of nude mice. Measurements were taken weekly, and tumor volumes were calculated using the formula V = length × width^2/2. The animals were sacrificed 4 weeks after injection. Pictures were recorded using a Nikon d800 digital camera (Nikon Corporation, Tokyo, Japan).