BALB/C nude male mice (six-week old) were purchased from Model Animal Research Center of Nanjing University. All animal experiments were conducted according to the National Institute of Health Guide for the Care and Use of Laboratory Animals and approved by the ethics committee of the Affiliated Zhongda Hospital of Southeast University. PC3 (5 × 106) cells which have been stably infected with LV-miR-193a or LV-NC were subcutaneously injected into each flank of the mice. Tumor size was measured every week with the following formula: (length × width2)/2. At the end of experiments, all mice were sacrificed and tumors were dissected and weighed. Partial xenograft tumor tissues were used for RNA extraction and detection of HOTAIR and miR-193a expression. And the rest tissues were formalin fixed immediately and paraffin embedded. Thereafter tissues were stained with antibody against Ki-67, CD31 and CD34 (all 1:100, Boster, Wuhan, China) according to the manufacturer’s instructions.
Antibody against ki 67
Manufactured by Boster Bio
Sourced in China
The Antibody against Ki-67 is a laboratory reagent used to detect the presence of the Ki-67 protein, which is a marker of cellular proliferation. This antibody can be utilized in various research applications, such as immunohistochemistry, to identify and quantify cells undergoing active cell division.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using antibody against ki 67
1
Nude Mice Xenograft Model for miR-193a
2
Evaluating PCa Cell Proliferation and Apoptosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The tumor tissues (both treatment and control group) were stained with antibody against Ki-67 (1:100; Boster Biological Technology, Ltd., Wuhan, People’s Republic of China) to evaluate the proliferation rate of PCa cells in vivo. Moreover, tumor tissue apoptotic cells were assessed by a terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay using an In Situ Cell Death Detection Kit (Roche, Mannheim, Germany). All images were captured using a microscope (Nikon). The apoptotic score was calculated by counting the number of positive nuclei in 30 random fields.
3
Protein Expression Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Antibodies against Paip1, cyclin B1, CDK1, P21, Bax, Bcl2, and actin were purchased from Proteintech Technology (Wuhan, China). Antibody against Ki-67 was purchased from BOSTER (Wuhan, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!