PE-conjugated anti-human CD33, APC-conjugated anti-human CD11b, PerCP-conjugated anti-human HLA-DR and the One-step Staining Human Treg Flow™ Kit were all purchased from BioLegend (USA). The samples were prepared according to the manufacturer’s instructions. The labeled cells were tested on a BD FACSCalibur cytometer and analyzed using FlowJo software version 7.6.1. (Tree Star, USA). In agreement with previous reports, MDSCs were defined as CD11b+/CD33+/HLA-DR- (14 (link)), and Treg cells were defined as CD4+/FoxP3+ (20 (link)). The labeling and gating strategies are shown in Figure 1 .
Pe conjugated anti human cd33
Manufactured by BioLegend
Sourced in United States
PE-conjugated anti-human CD33 is a fluorescent-labeled antibody that specifically binds to the human CD33 cell surface antigen. CD33 is a myeloid cell surface antigen expressed on myeloid-derived cells, including monocytes, granulocytes, and some leukemia cells.
Automatically generated - may contain errors
Lab products found in correlation
One step staining human treg flow kit, by BioLegend (1 mentions)
Apc conjugated anti human cd11b, by BioLegend (1 mentions)
Pe conjugated anti human cd8, by BD (1 mentions)
Accuri c6 plus, by BD (1 mentions)
Ab8216, by Abcam (1 mentions)
Apc conjugated anti human cd45, by BD (1 mentions)
Fitc conjugated anti mouse cd45, by BD (1 mentions)
Pe conjugated anti human cd19, by BioLegend (1 mentions)
2 protocols using pe conjugated anti human cd33
1
Identification of MDSC and Treg Cells
2
Immunophenotyping of Humanized Mice
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Blood, spleen, and BM cells from hu-mice were stained with antibodies and analyzed using a BD Accuri C6 Plus (BD Biosciences). The antibodies used to detect human cells included APC-conjugated anti-human CD45 (555485, BD PharmingenTM), FITC-conjugated anti-mouse CD45 (553080, BD PharmingenTM), APC-conjugated anti-human CD4 (555349, BD PharmingenTM), PE-conjugated anti-human CD8 (555367, BD PharmingenTM), PE-conjugated anti-human CD19 (302208, BioLegend), and PE-conjugated anti-human CD33 (303403, BioLegend). For immunocy-tochemistry, anti-CD45 (ab8216, Abcam) and anti-CD34 (MAB72271, R&D Systems) antibodies were used, and proper isotype-matched IgG antibodies were used to detect the primary signals. Flow cytometric data were analyzed using the CSamplerTM Plus software program (BD Bio-sciences).
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