Total RNA extraction was performed as previously described [17 (link)] from 1 g of the frozen flavedo samples. The RNA concentration was measured by a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and its integrity was confirmed by migrating RNA (1 µg) on agarose gel [17 (link)]. RNA quality was verified by using the Agilent 2100 Total RNA Bioanalyzer (Agilent Technologies, Madrid, Spain) and the RNA 6000 Nano Kit (Agilent Technologies, Madrid, Spain) before the RNA-Seq analysis.
Agilent 2100 total rna bioanalyzer
Manufactured by Agilent Technologies
Sourced in Spain
The Agilent 2100 Total RNA Bioanalyzer is a laboratory instrument designed for the analysis of RNA samples. It provides quantitative and qualitative assessment of total RNA samples by separating and detecting the 18S and 28S ribosomal RNA (rRNA) peaks.
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Lab products found in correlation
2 protocols using agilent 2100 total rna bioanalyzer
1
RNA Extraction from Flavedo Samples
2
Total RNA Extraction and Quality Assessment
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Total RNA was extracted from 1 g of the flavedo or albedo samples, previously frozen, homogenized, and kept at −80 °C, as previously described [23 (link)]. The RNA concentration was determined by a NanoDrop ND-1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and its integrity was verified by migrating 1 µg of RNA on agarose gel [23 (link)]. Before the RNA-Seq analysis, RNA quality was assessed by the Agilent 2100 Total RNA Bioanalyzer (Agilent Technologies, Madrid, Spain) using the RNA 6000 Nano Kit (Agilent Technologies, Madrid, Spain).
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