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Mitopy1

Manufactured by Cayman Chemical
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Sourced in Israel

MitoPY1 is a fluorescent probe designed to detect mitochondrial hydrogen peroxide (H2O2) in living cells. It is a small-molecule dye that selectively locates in the mitochondria and its fluorescence intensity increases upon reaction with H2O2.

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Lab products found in correlation

Bibr 1532, by Bio-Techne (2 mentions) Mitosox, by Thermo Fisher Scientific (2 mentions) Endothelin 1, by Merck Group (1 mentions) Zln005, by Merck Group (1 mentions) Rotenone, by Merck Group (1 mentions)

3 protocols using mitopy1

1

Telomerase and Mitochondrial ROS Modulation

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The telomerase modulators BIBR-1532 (Tocris Bioscience) and AGS 499 (Ester Priel, PhD, Israel) and the mitochondrial ROS-detecting dyes MitoPY1 (Cayman chemicals) and mitoSox (life technologies) were prepared in DMSO. ET-1 (Peninsula Laboratories) was prepared in saline with 1% bovine serum albumin. All other chemicals were obtained from Sigma-Aldrich and prepared in distilled water or physiological salt solution. All concentrations represent the final concentrations in the organ bath.
Beyer A.M., Freed J.K., Durand M.J., Riedel M., Ait-Aissa K., Green P., Hockenberry J.C., Morgan R.G., Donato A.J., Peleg R., Gasparri M., Rokkas C.K., Santos J.H., Priel E, & Gutterman D.D. (2016). Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation. Circulation Research, 118(5), 856-866.
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2

Telomerase and Mitochondrial ROS Modulation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The telomerase modulators BIBR-1532 (Tocris Bioscience) and AGS 499 (Ester Priel, PhD, Israel), and the mitochondrial ROS-detecting dyes MitoPY1 (Cayman chemicals) and mitoSox (life technologies) were prepared in DMSO. ET-1 (Peninsula Laboratories) was prepared in saline with 1% bovine serum albumin. All other chemicals were obtained from Sigma-Aldrich and prepared in distilled water or physiological salt solution. Other agents were prepared in distilled water or physiological salt solution. All concentrations represent the final concentrations in the organ bath.
Beyer A.M., Freed J.K., Durand M.J., Riedel M., Ait-Aissa K., Green P., Hockenberry J.C., Morgan R.G., Donato A.J., Peleg R., Gasparri M., Rokkas C.K., Santos J.H., Priel E, & Gutterman D.D. (2016). Critical Role for Telomerase in the Mechanism of Flow-Mediated Dilation in the Human Microcirculation. Circulation Research, 118(5), 856-866.
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3

PGC-1α Activation and Mitochondrial Assessment

Cited 1 time
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ZLN005 (Sigma), a known small-molecule activator of PGC-1α,18 (link), 19 (link) was prepared in DMSO. Bio-Enhanced® Na-Rala (GeroNova Research) and dissolved in distilled water. Endothelin-1 (Sigma) was prepared in 1% bovine serum albumin. Lentiviral constructs (GFP and PGC-1α siRNA) were produced by the Blood Center of Wisconsin Hybridoma Core Lab and dissolved in distilled water. mitoPBA/mitoB (Cayman) was prepared in ethanol. Rotenone (Sigma) and MitoPY1 (Cayman) were prepared in DMSO.
Kadlec A.O., Chabowski D.S., Ait-Aissa K., Hockenberry J.C., Otterson M.F., Durand M.J., Freed J.K., Beyer A.M, & Gutterman D.D. (2017). PGC-1alpha Overexpression in Coronary Artery Disease Recruits Nitric Oxide and Hydrogen Peroxide during Flow-mediated Dilation and Protects Against Increased Intraluminal Pressure. Hypertension (Dallas, Tex. : 1979), 70(1), 166-173.
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