The following antibodies in our study were used for immunoblotting: Phospho-Mxi1 antibody, which was raised against peptide RIRMDS(p)IGSTISS by immunizing rabbits; anti-Mxi1 antibodies (sc-1042, Santa Cruz Biotechnology and HPA035319, Sigma-Aldrich); anti-S6K1 (sc-230), anti-Myc (sc-40), anti-Cdc20 (sc-13162), and anti-GAPDH (sc-166574) antibodies (Santa Cruz Biotechnology); anti-phospho-S6K1(Thr389) (#9234), anti-β-Trcp1 (#4394), anti-Cyclin B1(#4138), anti-Ubiquitin (#3933) and anti-HA (#3724) antibodies (Cell Signaling Technology); anti-Max (ab199489) , anti-β-Trcp2 (ab137770) and anti-Myc (ab32072) antibodies (Abcam); anti-RSK2 (23762-1-AP) and anti-GAPDH (60004-1-Ig) antibodies (Proteintech); anti-Flag (F1804), anti-Actin (A5441) and anti-Tubulin (T-5168) antibodies (Sigma-Aldrich). MG132 (474790, a proteasome inhibitor) was purchased from Millipore, Hydroxyurea (H8627) and CHX (01810, Cycloheximide, a protein synthesis inhibitor) were purchased from Sigma-Aldrich.
Anti gapdh sc 166574
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-GAPDH (sc-166574) is a primary antibody produced in mouse. It is designed to detect GAPDH (Glyceraldehyde 3-phosphate dehydrogenase) expression in various sample types.
Automatically generated - may contain errors
3 protocols using anti gapdh sc 166574
1
Immunoblotting Antibodies and Inhibitors
2
Chondrocyte Culture and Inflammatory Markers
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Chondrocyte growth media were purchased from Cell Signaling Technology (411–500, Beverly, MA, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and antibiotics (penicillin/streptomycin) were purchased from Welgene (Kyungsan, Korea). Anti-human MMP-1 (SC 58377) and anti-MMP-13 (SC 515284), anti-INOS (SC 650), anti-COX-2 (SC 1746), anti-IL-1β (SC 7884), and anti-GAPDH (SC 166574) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-MMP-3 antibody was purchased from R&D Systems (Minneapolis, MN, USA). The primers used in PCR analyses were obtained from Bioneer (Daejeon, Korea).
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Antibody Sources for Protein Analysis
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The human anti-Fbxw7α antibody was obtained from Abcam (ab12292). Human anti-SOX10 (sc-17342) anti-GSK3β (sc-53931) and anti-GAPDH (sc-166574) antibodies were obtained from Santa Cruz Biotechnology. Human anti-MIA (melanoma inhibitory activity) was purchased from Abnova (PAB27627) antibodies. Anti-HA (#3724), anti-Flag (#14793) and anti-Myc (#2278) were from Cell Signaling Technology. Bound primary antibodies were detected with either horseradish peroxidase-conjugated anti-mouse IgG HRP (SA00001–1, Proteintech, China) or horseradish peroxidase-conjugated anti-rabbit HRP (SA00001–2, Proteintech, China), and proteins were visualized by chemiluminescence.
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