Rat L6 myoblasts (JCRB9081) were purchased from JCRB cell bank (Osaka, Japan). Dulbecco’s modified eagle medium (DMEM), fetal bovine serum (FBS) and 0.02% EDTA were from MP Biomedical (CA, USA); penicillin, streptomycin, and 0.25% trypsin, from Invitrogen (CA, USA). Antibodies against AMPKα, phosphorylated AMPKα, ACC, phosphorylated ACC, and GLUT4 were purchased from Cell Signaling (MA, USA), antibodies against myoglobin, MEF2A, PGC-1α and Sp1 were from Santa Cruz Biotechnology (CA, USA), and α-tubulin antibody was from Wako Pure Chemical Industries Ltd. (Osaka, Japan). AMPK agonist 5-amino-4-imidazolecarboxamide-1-beta-D-ribofuranoside (AICAR) and AMPK inhibitor adenine 9-β-D-arabinofuranoside (araA) were purchased from Sigma-ALDRICH (MO, USA). AMPK inhibitor Compound C was purchased from Merck (DA, Germany)
Adenine 9 β d arabinofuranoside araa
Manufactured by Merck Group
Sourced in United States
Adenine 9-β-D-arabinofuranoside (araA) is a synthetic nucleoside analog used as a laboratory reagent. It functions as a base component for biochemical and molecular biology applications.
Automatically generated - may contain errors
Lab products found in correlation
Ampk inhibitor compound c, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Phosphorylated acc, by Cell Signaling Technology (1 mentions)
Celltiter glo luminescent cell viability assay kit, by Promega (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Bradford assay, by Bio-Rad (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium, by Thermo Fisher Scientific (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Compound c, by Merck Group (1 mentions)
Nrf2 sirna, by Thermo Fisher Scientific (1 mentions)
P ampk, by Cell Signaling Technology (1 mentions)
First strand cdna synthesis kit for rt pcr, by Thermo Fisher Scientific (1 mentions)
β actin a5441, by Merck Group (1 mentions)
Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions)
Tin protoporphyrin 9 snpp, by Santa Cruz Biotechnology (1 mentions)
2 protocols using adenine 9 β d arabinofuranoside araa
1
AMPK Signaling Pathway in Rat L6 Myoblasts
2
Molecular Mechanisms of HO-1 Regulation
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Fetal bovine serum, Dulbecco's modified Eagle's medium, trypsin/EDTA, penicillin-streptomycin, and TRIzol reagent were from Thermo Fisher Scientific (Carlsbad, CA, USA). LPS, Compound C and adenine 9-β-D-arabinofuranoside (Ara A) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Control small interfering RNA (siRNA), HO-1 siRNA, Nrf2 siRNA and Lipofectamine RNAiMax reagent were purchased from Thermo Fisher Scientific. Tin protoporphyrin-IX (SnPP) was from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Primary antibodies used are as follows: iNOS (sc-650), lamin B (sc-6216) and HO-1 (sc-10789) from Santa Cruz Biotechnology; NFκB (NBP1-96139) from Novus Biologicals (Littleton, CO, USA); IκB (#9242), p-IκB (#2859), AMPK (#2532) and p-AMPK (#2535) from Cell Signaling Technology (Danvers, MA, USA); and β-actin (A5441) from Sigma-Aldrich. Anti-rabbit IgG, anti-goat IgG and anti-mouse IgG were purchased from Sigma-Aldrich. First Strand cDNA Synthesis kit for RT-PCR was from MBI Fermentas (Ontario, Canada). Protein concentration of samples was determined by Bradford assay (Biorad, Hempstead, UK). Enhanced luminal-based chemiluminescence western blotting detection system was obtained from Pierce Chemical (Rockford, IL, USA). CellTiter-Glo® Luminescent Cell Viability Assay kit was from Promega (Madison, WI, USA).
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