Peroxidase labeled secondary antibodies

Manufactured by Proteintech

Peroxidase-labeled secondary antibodies are conjugated with the enzyme horseradish peroxidase. They are used to detect and amplify the signal of primary antibodies in various immunoassays, such as Western blotting, ELISA, and immunohistochemistry.

Automatically generated - may contain errors

Lab products found in correlation

Myc tag antibody, by Proteintech (2 mentions) Supersignal west dura extended duration substrate, by Thermo Fisher Scientific (2 mentions) Noxa antibody, by Abbkine (2 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti e cadherin ab1416, by Abcam (1 mentions) Radioimmunoprecipitation assay (ripa), by Beyotime (1 mentions) Anti gapdh ap0063, by Bioworld Technology (1 mentions) Fitc conjugated goat anti mouse antibody, by Thermo Fisher Scientific (1 mentions) Bak antibody, by Abcam (1 mentions)

3 protocols using peroxidase labeled secondary antibodies

Protein Extraction and Western Blot Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were harvested in phosphate-buffered saline (PBS) and centrifuged for 1 minute at 13,000 rpm. The pellet was resuspended in 100 μL of lysis buffer (radio-immunoprecipitation assay [Beyotime, Shanghai, People’s Republic of China], Cocktail, and phenylmethanesulfonyl fluoride [Hoffman-La Roche Ltd., Basel, Switzerland]) and incubated for 30 minutes on ice. The lysate was centrifuged for 10 minutes at 12,000 rpm at 4°C. The protein amount was determined using the bovine serum albumin (BSA) kit (Beyotime). For immunoblotting, the following antibodies were used: anti-MAP2K7 (55030-1-Ap; Proteintech, Rosemont, IL, USA), anti-E-cadherin (ab1416; Abcam, Cambridge, UK), anti-N-cadherin (4061; cell signaling technology), and anti-GAPDH (AP0063; Bioworld Technology, St Louis Park, MN, USA). Bound antibodies were detected by using peroxidase-labeled secondary antibodies (Proteintech) and immunoblotting and enhanced chemiluminescence detection reagents from Thermo Fisher Scientific.

Hong L., Pan F., Jiang H., Zhang L., Liu Y., Cai C., Hua C., Luo X., Sun J, & Chen Z. (2016). miR-125b inhibited epithelial–mesenchymal transition of triple-negative breast cancer by targeting MAP2K7. OncoTargets and therapy, 9, 2639-2648.

+ Open protocol

+ Expand

Western Blot Analysis of Key Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five micrograms of protein were electrophoresed in 10% SDS-PAGE gels and blotted to polyvinylidene difluoride membranes. Specific primary antibodies were detected with peroxidase-labeled secondary antibodies (ProteinTech Group Inc.) by using SuperSignal West Dura Extended Duration Substrate (Pierce Chemical) per the manufacturer’s instructions. The antibodies used from ProteinTech Group Inc. included the myc-tag antibody (Cat. #66036–1-Ig), ASS antibody (Cat. #66036–1-Ig), GAPDH antibody (Cat. #60004–1-Ig), FTL antibody (Cat. #10727–1-AP), Flag-tag antibody (Cat. # 66008–3-Ig), p53 antibody (Cat. #60283–2-Ig), Bcl-2 antibody (Cat. #60178–1-Ig), PUMA antibody (Cat. # 55120–1-AP), Bax antibody (Cat. #60267–1-Ig), caspase 9 antibody (Cat. # 66169–1-Ig), caspase 3 antibody (Cat. # 66470–2-Ig), Histone H3 antibody (Cat. # 17168–1-AP), HRP-conjugated goat anti-mouse IgG (Cat. #SA00001–1) and HRP-conjugated goat anti-rabbit IgG (Cat. #SA00001–2). The p53AIP1 antibody (Cat. # ABP56144) was supplied by Abbkine Inc., while the Noxa antibody (Cat. # ab13654) and the Bak antibody (Cat. # ab69404) were both from Abcam Inc. The TRITC conjugated goat anti-rabbit antibody (Cat. # AS10–1018) was from Agrisera Inc.

Feng Q., Bian X., Liu X., Wang Y., Zhou H., Ma X., Quan C., Yao Y, & Zheng Z. (2020). Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer, 20, 665.

+ Open protocol

+ Expand

Comprehensive Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Five micrograms of protein were electrophoresed in 10% SDS-PAGE gels and blotted to polyvinylidine difluoride membranes. Specific primary antibodies were detected with peroxidase-labeled secondary antibodies (ProteinTech Group Inc.) by using SuperSignal West Dura Extended Duration Substrate (Pierce Chemical) per manufacturer's instructions. The used antibodies from ProteinTech Group Inc. included myc-tag antibody (Cat. #16286-1-AP), ASS antibody (Cat. #66036-1-Ig), GAPDH antibody (Cat. #60004-1-Ig), Flag-tag antibody (Cat. #66008-3-Ig), p53 antibody (Cat. #60283-2-Ig), Bcl-2 antibody (Cat. #60178-1-Ig), PUMA antibody (Cat. #55120-1-AP), Bax antibody (Cat. #60267-1-Ig), caspase 9 antibody (Cat. #66169-1-Ig), caspase 3 antibody (Cat. #66470-2-Ig), Histone H3 antibody (Cat. #17168-1-AP), HRP-conjugated goat anti-mouse IgG (Cat. #SA00001-1) and HRP-conjugated goat anti-rabbit IgG (Cat. #SA00001-2). FTL antibody (Cat. #ab201975) was from Abcam Inc.. p53AIP1 antibody (Cat. #ABP56144) was from Abbkine Inc., Noxa antibody (Cat. #ab13654) was from Abcam Inc.. Bak antibody (Cat. #ab69404) was from Abcam Inc.. TRITC conjugated goat anti-rabbit antibody (Cat. #A16101) was from ThermoFisher Scientific Inc.. FITC conjugated goat anti-mouse antibody (Cat. #62-6511) was from ThermoFisher Scientific Inc..

Feng Q., Bian X., Luo X., Wang Y., Zhou H., Ma X., Quan C., Yao Y., & Zheng Z. (2020). Intracellular expression of arginine deimimase activates the mitochondrial apoptosis pathway through inhibiting cytosolic ferritin and inducing chromotin autophagy.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!