Itc annexinv propidiuiodide

Manufactured by BestBio

Sourced in China

The ITC AnnexinV/propidiuiodide is a laboratory equipment used for cell analysis. It measures the binding of AnnexinV and propidium iodide to cells, which can provide information about cell apoptosis and viability.

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2 protocols using itc annexinv propidiuiodide

Molecular Mechanisms of Cell Injury

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Antibodies RIP1, RIP3, KIM-1, Nox2, Nox4, TNF-α, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA); Rabbit anti-P-MLKL and Anti-cleaved-caspase-3, cleaved-caspase-12 were obtained from Cell Signaling Technology (CST, Danvers, MA, USA). Lipofectamine 3000 was purchased from Science Biotechnology (Invitrogen, Beijing, China). Protein Assay Kit was purchased from Beyotime Institute of Biotechnology (Jiangsu, China). Cell Malondialdehyde (MDA) assay kit (Colorimetric method), reduced glutathione (GSH) assay kit, creatinine (Cr) Assay kit (sarcosine oxidase) and urea (BUN) assay kit were obtained from Nanjing Jiancheng Bioengineering Institute(Nanjing, China). Reactive Oxygen Species Assay (DCF Assay) Kit and Dihydroethidium (DHE) were purchased from Beyotime Institute of Biotechnology (Jiangsu, China). ITC AnnexinV/propidiuiodide was purchased from Bestbio (Shanghai, China). Mouse NGAL ELISA kit was purchased from CUSABIO (Wuhan, China).

Gao L., Wu W.F., Dong L., Ren G.L., Li H.D., Yang Q., Li X.F., Xu T., Li Z., Wu B.M., Ma T.T., Huang C., Huang Y., Zhang L., Lv X., Li J, & Meng X.M. (2016). Protocatechuic Aldehyde Attenuates Cisplatin-Induced Acute Kidney Injury by Suppressing Nox-Mediated Oxidative Stress and Renal Inflammation. Frontiers in Pharmacology, 7, 479.

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Investigating Necroptosis Pathway Regulation

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Antibodies RIPK1, RIPK3, β-actin, and OCT2 were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA); anti-E-cadherin, anti-P-P65 and anti-P65 were purchased from Cell Signaling Technology (CST, Danvers, Biotechnology (Invitrogen, Beijing, China). Necrostatin-1 (Nec-1) and z-VAD were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Human recombinant TNF-α was purchased from R&D systems (R&D Systems Inc, MN, USA). Protein Assay Kit was from Beyotime Institute of Biotechnology (Jiangsu, China). Creatinine and blood urea nitrogen (BUN) kits were obtained from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). ITC AnnexinV/propidiuiodide was purchased from Bestbio (Shanghai, China). Dual-Luciferase® Reporter Assay System was from Promega Corporation.

Gao L., Liu M.M., Zang H.M., Ma Q.Y., Yang Q., Jiang L., Ren G.L., Li H.D., Wu W.F., Wang J.N., Wei B., Liu X.Q., Jiang C., Huang C., Li J, & Meng X.M. (2018). Restoration of E-cadherin by PPBICA protects against cisplatin-induced acute kidney injury by attenuating inflammation and programmed cell death. Laboratory investigation; a journal of technical methods and pathology, 98(7).

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