Thy 1 antibody

Manufactured by Santa Cruz Biotechnology

The Thy-1 antibody is a protein that binds to the Thy-1 cell surface antigen. It is commonly used in research applications to identify and isolate Thy-1 positive cells, which are often associated with certain cell types and developmental stages.

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Lab products found in correlation

Alexa fluor 594 donkey anti rabbit igg, by Thermo Fisher Scientific (1 mentions) Cd105, by Merck Group (1 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

2 protocols using thy 1 antibody

Mesenchymal Stem Cell Mobilization by KLD12-SP

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Example 6

Since substance P of KLD12-SP is expected to have the ability to mobilize mesenchymal stem cells, immunofluorescent staining was performed using CD90 (Thy-1 antibody, Santa Cruz Biotechnology) and CD105 (anti-endoglin monoclonal antibody, Millipore, Temecula, Calif.), which are markers specifically expressed in mesenchymal stem cells, in the groups treated with KLD12-SP-MSC and KLD12-SP without MSC. Alexa Fluor 594 donkey anti-rabbit IgG (Invitrogen) and Alexa Fluor 488 goat anti-mouse IgG (Invitrogen) were used as secondary antibodies and counterstained with DAPI to observe cell nuclei. The results are shown in Fig.

As shown in FIG. 8, the expression of CD90 and CD105, which are mesenchymal stem cell markers, was at least twice that of the control group in the group treated with KLD12-SP without MSC. These results indicate that KLD12-SP has the ability to mobilize mesenchymal stem cells to the arthritic sites, and it was determined that the regeneration of chondrocytes can be performed more actively by the mesenchymal stem cells migrating to the arthritic sites.

US10434135B2. Pharmaceutical composition for preventing or treating arthritis (2019-10-08). Samsung Life Public Welfare Foundation [KR], Korea Institute of Science and Technology [KR]. Inventors: Sang Jun Kim [KR], Young Mee Jung [KR], Soo Hyun Kim [KR].

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Profiling Cell and Tissue Lysates using Western Blot

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Lysates of cultural cells (BMSCc and MCF7) and tissues (mouse brain at postnatal day 1 and adult rat DRGs) were extracted using 1× RIPA buffer (20 mm Tris–HCl pH 7.4, 150 mm NaCl, 1% Nonidet P-40, 1% sodium deoxycholate, 0.1% SDS, with 0.1% Triton X100 and protease inhibitor cocktail). Protein concentration was determined by using the BCA kit (Pierce, Rockford, IL). Western blotting (20 μg protein) was preceded by SDS-PAGE gel electrophoresis, transferred onto nitrocellulose, and probed with Thy-1 antibody (1:100, Santa Cruz Biotechnology, SCB, Santa Cruz, CA) and Stro1 antibody (1:100, Life Technologies). Immunoreactive proteins were detected by enhanced chemiluminescence (Pierce, Rockford, IL) after incubation with HRP-conjugated second antibodies (1:2000, SCB) and exposed to photographic film. GAPDH (1:2000, Cell Signaling, Danvers, MA) was used as a loading control.

Fischer G., Wang F., Xiang H., Bai X., Yu H, & Hogan Q.H. (2017). Inhibition of neuropathic hyperalgesia by intrathecal bone marrow stromal cells is associated with alteration of multiple soluble factors in cerebrospinal fluid. Experimental brain research, 235(9), 2627-2638.

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