Dihydrexidine

Manufactured by Bio-Techne

Dihydrexidine is a selective dopamine D1 receptor agonist. Its core function is to activate and stimulate the D1 receptor subtype of the dopamine receptor family.

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Lab products found in correlation

5 protocols using dihydrexidine

Preparation of D1 Receptor Ligands

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SCH23390 (Tocris, Cat. #: 0925), a standard selective D1-like antagonist, was prepared as a 100 mM stock solution in saline and delivered at 1 mM final concentration in saline for cannulation experiments. The potent full efficacy D1R agonist Dihydrexidine (Tocris, Cat. #: 0884) was prepared as a 10 mM stock solution in deionized water.

Park A.J., Harris A.Z., Martyniuk K.M., Chang C.Y., Abbas A.I., Lowes D.C., Kellendonk C., Gogos J.A, & Gordon J.A. (2021). Reset of hippocampal-prefrontal circuitry facilitates learning. Nature, 591(7851), 615-619.

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In Vitro Midbrain Neural Progenitor Culture

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Brains from E11.5 mice were obtained and the midbrain region was dissected, mechanically dissociated, plated on poly-d-lysine (150,000 cells/cm²), and grown in serum-free N2 media consisting of F12/DMEM (1:1) with insulin (10 ng/ml), apo-transferrin (100 μg/ml), putrescine (100 μM), progesterone (20 nM), selenium (30 nM), glucose (6 mg/ml), BSA (1 mg/ml) and FGF2 (10 ng/ml). Cells were treated for 3 days in vitro with either dopamine (10 μM, Sigma), haloperidol (1 μM, Sigma), quinpirole (10μM, Tocris Bioscience), dihydrexidine (10 μM, Tocris Bioscience), muscimol (30 μM, Sigma) sulpiride (10 μM, Tocris Bioscience), SCH-23390 (10 μM, Santa Cruz Biotechnology), picrotoxin (30 μM, Tocris Bioscience) or media only and then processed for immunocytochemistry. For BrdU analysis, cells were treated with BrdU 1 hour after plating. After a further 2 days in culture, cells were treated for 30 minutes with 2N HCl, and processed for immunocytochemistry.

Hedlund E., Belnoue L., Theofilopoulos S., Salto C., Bye C., Parish C., Deng Q., Kadkhodaei B., Ericson J., Arenas E., Perlmann T, & Simon A. (2016). Dopamine Receptor Antagonists Enhance Proliferation and Neurogenesis of Midbrain Lmx1a-expressing Progenitors. Scientific Reports, 6, 26448.

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Screening Compounds for Neurotransmitter Receptor Interactions

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All other screening compounds, except m-CPP (Sigma-Aldrich) were from Tocris Bioscience: Sumanirole (D₂ agonist), Spiperone (5-HT_2A serotonin and selective D₂-like dopamine receptor antagonist), Lisuride (D_2∼4 agonist), Pramipexole (D_2∼4 agonist), BW723C86 (5-HT_2B receptor agonist), m-CPP (Pan-serotonin agonist), Dihydrexidine (full agonist at the dopamine D1 and D5 receptors), Cabergoline (D_2∼4 agonist, possible prolactin inhibitor), DL-TBOA (EAAT blocker)

Liberante F.G., Pouryahya T., McMullin M.F., Zhang S.D, & Mills K.I. (2015). Identification and validation of the dopamine agonist bromocriptine as a novel therapy for high-risk myelodysplastic syndromes and secondary acute myeloid leukemia. Oncotarget, 7(6), 6609-6619.

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Preparation of D1 Receptor Ligands

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Pharmacological Compounds for Cell Experiments

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Y-27632, forskolin, SB431542, formoterol, fenoterol, salbutamol, salmeterol, and fenoldopam were purchased from Cayman Chemical (Ann Arbor, MI). Dihydrexidine, A77636, A68930, and fenoldopam were purchased from Tocris Bioscience (Minneapolis, MN). Recombinant human TGF-β1 was purchased from PeproTech (Rocky Hill, NJ). Bovine serum albumin (BSA), 10% formalin and triton X-100 were purchased from Sigma-Aldrich (St. Louis, MO). Phosphate buffered saline (PBS) was purchased from Life Technologies (Carlsbad, CA).

Gao A.Y., Diaz Espinosa A.M., Nguyen B.B.N., Link P.A., Meridew J., Jones D.L., Gibbard D.F., Tschumperlin D.J, & Haak A.J. (2023). Dopamine Receptor D1 Is Exempt from Transforming Growth Factor β-Mediated Antifibrotic G Protein-Coupled Receptor Landscape Tampering in Lung Fibroblasts. The Journal of pharmacology and experimental therapeutics, 386(3).

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