Anti fadd 610400

TNF-α and z-VAD-fmk were purchased from R&D. Cyclohexamide (CHX) from Sigma. Smac mimetic was a gift from S. Wang (University of Michigan, Ann Arbor, Michigan, USA). TRAIL from Novoprotein. FasL from Upstate Biotech. CH11 from MBL Medical and Biol Lab.
All antibodies are at concentration of 1 μg ml⁻¹ and used at 1:1,000 dilution unless otherwise stated. Anti-RARγ (C-15) (sc-550) for human, anti-RARα (C-20) (sc-551), anti-caspase-8 (C-20) (sc-6136), anti-cIAP2 (sc7944) and anti-Fas (C-20) (sc-715) from Santa Cruz; anti-RIP1 (610459) and anti-FADD (610400) from BD Biosciences; anti-RARγ1 (ab5905) for mouse, anti-RIP3 (ab72106), anti-MLKL (ab184718) for human, anti-MLKL (ab172868) for mouse and anti-cIAP1 (ab2399) from Abcam; anti-RIP3 (2283) for mouse from ProSci, anti-TRADD (05-473) from Upstate; anti-TRAF2 (MAB3277) and anti-TNFR1 (AF-425-PB) from R&D; anti-Actin (A3853) (dilution 1:10,000), anti-FLAG (F9291) (dilution 1:5,000) and anti-GFP (G6539) (dilution 1:5 000) from Sigma; anti-V5 (R960-25) (dilution 1:5,000) from Invitrogen; anti-PARP1 (BML-SA250-0050) from Enzo Life Science; anti-GAPDH (NB300-22) (dilution 1:5,000) from Novus Biologicals; anti-cleaved caspase-8 (9496), anti-CYLD (4495), anti-RIP1 (137451) and anti-p-RIP1(65746) from Cell Signaling Technology; anti-DsRed (632392) (dilution 1:5,000) from Clontech.

Pan-caspase inhibitor, z-VAD-FMK (carbobenzoxy-valyl-alanyl-aspartyl-[O-methyl]- fluoromethylketone), GSK’782, and necrosulfonamide (NSA) were purchased from Calbiochem (Merck Millipore, Darmstadt, Germany). Gemcitabine and Necrostatin-1 (Nec-1) were from Sigma (St Louis, Missouri, USA). TNF-α was from R&D systems (Minneapolis, Minnesota, USA). Smac mimetic (SM-164) was a gift from S. Wang (University of Michigan, Ann Arbor, Michigan, USA). Antibodies for Western blot were obtained from commercial sources: anti-RIPK1 (610459) and anti-FADD (610400) were from BD Biosciences (San Jose, California, USA); anti-RIPK3 (8457), anti-cIAP1 (7065), anti-cIAP2 (3130), anti-NIK (4994), anti-cyclin D1 (2978), anti-caspase-8 (9746) and anti-actin (4970) were from Cell Signaling (Danvers, Massachusetts, USA); anti-MLKL (ab184718) and anti-phosphorylated MLKL (ab187091) were from Abcam (Cambridge, UK).