Serum alanine aminotransferase (ALT) was determined using a kinetic method (D-TEK, Bensalem, PA, USA). Liver triglyceride levels were assessed using the L-Type Triglyceride H kit (Wako Chemicals USA Inc., VA, USA). Serum and liver TNFα (BioLegend Inc., San Diego CA, USA), IL-1β (R&D Systems, Minneapolis, MN, USA) and MCP1 (BioLegend Inc., San Diego CA, USA) levels were determined by ELISA as described by manufactures.
L type triglyceride h kit
Manufactured by Fujifilm
Sourced in United States
The L-Type Triglyceride H kit is a laboratory reagent used for the quantitative determination of triglycerides in biological samples. It provides a colorimetric assay for the measurement of triglyceride levels.
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Lab products found in correlation
4 protocols using l type triglyceride h kit
1
Serum and Liver Biomarker Quantification
2
Plasma and Hepatic Lipid Profiling in Mice
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Blood samples from anesthetized mice were collected by puncturing vena cava using 1 cc insulin syringe containing 50 μL of 1 mM ethylenediaminetetraacetic acid in the absence of protease inhibitors. Plasma levels of triglyceride, cholesterol, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were measured using a blood chemistry analyzer.
For analysis of hepatic triglyceride and nonesterified fatty acids (NEFA), liver tissues were ground in liquid nitrogen and dissolved in 0.9% sodium chloride. The NEFA levels were determined from the supernatant using the commercial enzymatic colorimetric kits (Wako Chemicals, VA, USA), according to the manufacturer's instructions. The liver triglyceride levels were determined using an L-type triglyceride H kit (Wako Chemicals, VA, USA), according to the manufacturer's instructions.
For analysis of hepatic triglyceride and nonesterified fatty acids (NEFA), liver tissues were ground in liquid nitrogen and dissolved in 0.9% sodium chloride. The NEFA levels were determined from the supernatant using the commercial enzymatic colorimetric kits (Wako Chemicals, VA, USA), according to the manufacturer's instructions. The liver triglyceride levels were determined using an L-type triglyceride H kit (Wako Chemicals, VA, USA), according to the manufacturer's instructions.
3
Serum ALT and Liver Triglycerides in Mice
4
Hemodialysis Lipid Profile Dynamics
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All patients began the HD sessions in the morning and consumed a meal within two hours of the start of HD. Blood samples were collected during the first hemodialysis session of the week. The serum albumin and C-reactive protein (CRP) levels were measured in the blood samples collected at the beginning of HD. We also evaluated the singlepool Kt/V values using the formula reported by Shinzato et al. (21) . The serum total cholesterol, HDL cholesterol, TG and total protein levels were measured at zero, two and four hours after the start of each single HD session. The serum total cholesterol levels were measured using the Biancore Liquid T-CHO II kit (TOYOBO, Osaka, Japan), the TG levels were measured using the L Type Triglyceride H kit (Wako, Osaka, Japan) and the HDL cholesterol levels were measured using the METABOLEAD HDL-C kit (Kyowa Medex, Tokyo, Japan). The lipid parameters were measured three times during three separate hemodialysis sessions, and the mean values were calculated for use in the analyses.
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