2 hydroxypropyl γ cyclodextrin hpγcd

Cell culture media and reagents were purchased from Thermo Fisher Scientific (Waltham, MA, USA). These include Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin. A CellTiter 96 Aqueous One Solution Cell Proliferation Assay System was purchased from Promega (Madison, WI, USA). U18666A, Filipin III, 2-hydroxypropyl-β-cyclodextrin (HPβCD) and 2-hydroxypropyl-γ-cyclodextrin (HPγCD) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies used in this study are as follows: rabbit polyclonal antibody for mCherry (Abcam ab183628); rabbit monoclonal antibody for GAPDH (Cell Signaling #5174).

Natamycin was purchased from LabNetwork^® (Saint Paul, MN, USA); Voriconazole was procured by Normon^® (Madrid, Spain); Hyaluronic Acid (HA) (MW 1.4 × 10⁶ Da) was obtained from Acofarma^® (Barcelona, Spain); 2-hydroxypropyl-β-cyclodextrin (HPβCD) (Kleptose^®, 0.65 molar substitution ratio, MW 1399 Da) was obtained from Roquette^® Laisa S.A. (Valencia, Spain); 2-hydroxypropyl-γ-cyclodextrin (HPγCD) (0.6 molar substitution ratio, MW 1580 Da) was purchased from Sigma Aldrich^® (Darmstadt, Germany); Liquifilm^® was obtained from Allergan^® Pharmaceuticals Ireland (Mayo, Ireland); Poloxamer (P407) and Polyvinyl alcohol (PVA) (Mw 30,000–70,000 Da) were procured by Sigma Aldrich; and Methyl Cellulose (MC) (1500 cP) was procured by Shin-Etsu (Japan).