Tubp gal4

Manufactured by Bloomington Drosophila Stock Center

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The TubP-GAL4 is a genetic tool used in the study of Drosophila melanogaster. It is a driver line that expresses the GAL4 transcription factor under the control of the tubulin promoter, which provides ubiquitous expression throughout the organism's development and lifespan.

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6 protocols using tubp gal4

Genetic Manipulation of Drosophila Stocks

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Fly stocks were cultivated on standard media in uncrowded conditions at 25°C unless otherwise stated. The following stocks were obtained from Bloomington Stock Center: Tub-GAL4 (P{w^+mC= tubP-GAL4}LL7 originally from stock 5138, y¹w^*;; P{w^+mC= tubP-GAL4}LL7/TM6b,GFP), act5C-GAL4 (stock 4414, y¹w^*; P{w^+mC= act5C-GAL4}25FO1/CyO, y⁺;), nub-GAL4 (stock 25754; P{UAS-Dcr-2.D}1, w¹¹¹⁸; P{GawB}nub-AC-62), 69B-GAL4 (stock 1774; w*;;P{GawB}69B), UAS-idgf1^RNAi (stock 57508, y¹sc^*v¹; P{TRiP.HMC04823}attP40), UAS-idgf2^RNAi (stock 55935, y¹sc^*v¹; P{TRiP.HMC04223}attP40), UAS-idgf3^RNAi (stock 67226, y¹sc^*v¹; P{TRiP.HMC06327}attP40), Df(3L)Exel6084 (stock 7563, w¹¹¹⁸;; Df(3L)Exel6084, P{XP-U}Exel6084/TM6B). Stocks obtained from the Vienna Drosophila Resource Center were: UAS-dis3L2^RNAi (stock v51854, w¹¹¹⁸; P{GD9240}v51854; and stock v100322,;; P{KK105902}VIE-260B) and Dis3L2^CD (stock 312503). en-GAL4 (A kind gift from Paul Martin,; engrailed-GAL4,UAS-GFPactin/Cyo;). To overexpress Dis3L2 the stock w*;; P{GSV3}GS6090/TM6 was purchased from the Kyoto Stock Center (stock 200902), however, a mutation outside the Df6084 locus caused homozygous lethality; this was repaired by recombination.

Towler B.P., Pashler A.L., Haime H.J., Przybyl K.M., Viegas S.C., Matos R.G., Morley S.J., Arraiano C.M, & Newbury S.F. (2020). Dis3L2 regulates cell proliferation and tissue growth through a conserved mechanism. PLoS Genetics, 16(12), e1009297.

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Rearing Drosophila Flies for Genetic Studies

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Flies were reared on cornmeal/molasses/agar medium under standard culture conditions (29°C, 25°C or 18°C depending on the presence of tubP-Gal80^ts, 12 h:12 h light/dark cycle). CO₂ was used as an anesthetic. slgA^NP4104, UAS-mCD8-gfp, UAS-mito-tomato, UAS-RFP, CkIIα^JF01436, CkIIα ^GL0003, slgA^GL01514, TubP-Gal80^ts, TubP-Gal4, OK107-Gal4 and 201y-Gal4 were obtained from the Bloomington Drosophila Stock Center, Bloomington, IN, USA. P{cry-Gal4.E39} and P{pdf-Gal4.P2.4} were a gift of Dr Bassem Hassan (ICM, Paris, France). All fly stocks were isogenized by mating females to Canton-S males for ten generations to exclude effects due to differences in genetic background. The RNAi lines used were predicted to have no off-target effects (Ni et al., 2009 (link)).

Zwarts L., Vulsteke V., Buhl E., Hodge J.J, & Callaerts P. (2017). SlgA, encoded by the homolog of the human schizophrenia-associated gene PRODH, acts in clock neurons to regulate Drosophila aggression. Disease Models & Mechanisms, 10(6), 705-716.

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Drosophila Genetic Toolkit Utilization

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The following fly stocks were obtained from Bloomington Stock Center: DJ667 GAL4 (stock #8171), Act5C-GAL4 (stock #4414), tubP-GAL4 (stock #5138), longGMR-GAL4 (stock 8605), nanos-GAL4:VP16 (stock #7312).

Jones T.I., Parilla M, & Jones P.L. (2016). Transgenic Drosophila for Investigating DUX4 and FRG1, Two Genes Associated with Facioscapulohumeral Muscular Dystrophy (FSHD). PLoS ONE, 11(3), e0150938.

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Drosophila Lines for Cholinergic and Neuronal Expression

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Flies carrying the UAS-mouse (Mo)PrP insert at attP2 have been previously described (Murali et al. 2014 (link)). The GAL4 driver lines w¹¹¹⁸;P{ChAT-GAL4.7.4}/CyO,P{sevRas1.V12}FK1, w*;P{GAL4-elav.L}3, and y¹ w*;P{tubP-GAL4}LL7/TM3, Sb¹ Ser¹, referred to as Cha-GAL4, Elav-GAL4, and α-tubulin-GAL4, respectively, were obtained from the Bloomington Drosophila Stock Center (BDSC) (Bloomington, IN). Cha-GAL4 drives UAS-tagged transgene expression in cholinergic neurons at all developmental stages (Salvaterra and Kitamoto 2001 (link)), while Elav-GAL4 drives transgene expression in all postmitotic neurons as well as in certain neuroblasts and embryonic glial cells (Berger et al. 2007 (link); Robinow and White 1988 (link)). Flies were maintained at 25°, 70% relative humidity on standard Drosophila cornmeal, yeast, molasses, and agar medium with methylparaben as a mold inhibitor.

Noble G.P., Dolph P.J, & Supattapone S. (2016). Interallelic Transcriptional Enhancement as an in Vivo Measure of Transvection in Drosophila melanogaster. G3: Genes|Genomes|Genetics, 6(10), 3139-3148.

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Genetic tools for Drosophila Wnt signaling

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UAS-GFP-wg[27] (link), UAS-DFz2 and UAS-DFz2N[28] (link), UAS-Dlp-HA[16] (link), wg{KO; Gal4}[29] (link), wg{KO; wg-HA}[30] (link), wg^cx4, sfl⁰³⁸⁴⁴, Df(3R)Exel6193, Df(3R)BSC527, Df(3R)BSC619, Mi{ET1}CG13830^MB00767, neur-lacZ (neur^A101), UAS-Shi^ts, UAS-lacZ, UAS-myrRFP, UAS-FLP, tub-p-Gal4, ap-Gal4, en-Gal4, hh-Gal4, ptc-Gal4, MS1096-Gal4, and nub-Gal4 were obtained from the Bloomington Drosophila Stock Center. The cow^5Δ allele was generated by imprecise excision of the Minos transposable element Mi{ET1}CG13830^MB00767 from the 3′-UTR of cow (see Figure S1A).

Chang Y.H, & Sun Y.H. (2014). Carrier of Wingless (Cow), a Secreted Heparan Sulfate Proteoglycan, Promotes Extracellular Transport of Wingless. PLoS ONE, 9(10), e111573.

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Transgenic Fly Development via PhiC31 Integration

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Transgenic flies were developed using codon optimized Sc-FAR-1 or Sc-FAR-2 inserted via the PhiC31 site-specific serine integrase method. The transgenic UAS lines were then crossed with several Gal4 drivers from the Bloomington Drosophila Stock Center (BDSC), including TubP-Gal4 (#5138; strong, ubiquitous somatic expression), CG-Gal4 (#7011; expressed in the fat body, hemocytes and lymph gland) and He-Gal4 (#8699; expressed in hemocytes). Fly husbandry and crosses were performed under standard conditions at 25°C. Rainbow Transgenics (Camarillo, CA) carried out all of the fly injections. All constructs were inserted into attP line 86Fa (BDSC #24486: y[1 (link)] M{vas-int.Dm}ZH-2A w[*]; M{3xP3-RFP.attP’}ZH-86Fa).

Parks S.C., Nguyen S., Nasrolahi S., Bhat C., Juncaj D., Lu D., Ramaswamy R., Dhillon H., Fujiwara H., Buchman A., Akbari O.S., Yamanaka N., Boulanger M.J, & Dillman A.R. (2021). Parasitic nematode fatty acid- and retinol-binding proteins compromise host immunity by interfering with host lipid signaling pathways. PLoS Pathogens, 17(10), e1010027.

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