GC–MS analysis was performed on an Agilent 7890/5975C GC–MS (Agilent Technologies, Santa Clara, CA, USA), with chromatographic separation achieved using a DB-5 fused silica capillary column (30 m × 0.25 mm × 0.25 μm) (J&W Scientific, Folsom, CA, USA). The flow rate of the carrier gas (high-purity helium, 99.9996%) was 1.2 mL/min, and the column oven heating was programmed as follows: after initial holding at 70°C for 3 min, the temperature was increased to 300°C at a rate of 5°C/min, and kept it maintained there for 5 min. The injection volume and split ratios were 1 μL and 5:1, respectively, while the injection port and transfer line temperatures were 300 and 280°C, respectively. The ion source (EI) temperature was 230°C, whereas the mass spectrum scan range and solvent delay time were m/z 33~600 and 4.8 min, respectively. All samples were analyzed in a random order, and QC samples were injected within the sequence.
Agilent 7890 5975c gc ms
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 7890/5975C GC–MS is a gas chromatography-mass spectrometry system designed for high-performance chemical analysis. It combines a gas chromatograph with a mass spectrometer to enable the separation, identification, and quantification of chemical compounds in complex samples.
Automatically generated - may contain errors
5 protocols using agilent 7890 5975c gc ms
1
GC-MS Analysis of Organic Compounds
2
Hydrogenation of 2,3-Dimethylindole
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
2,3-dimethylindole (AR) and Mesitylene (AR) were provided by Energy Chemical Company and Sinopharm Chemical Reagent Company Limited, respectively. 5 wt% Ru/Al2O3 and 5 wt% Pd/Al2O3 catalysts were purchased from Shanxi Kaida Chemical Engineering Company Limited. The BET surface areas and pore sizes of the Ru/Al2O3 and Pd/Al2O3 are 85.24 m2 g−1, 13.41 nm and 68.35 m2 g−1, 11.89 nm, which are measured by ASAP 2020. Ultra-high purity H2 gas was obtained from Sichuan Ally High-Tech Company. Gas chromatography and mass spectrometry (Agilent 7890/5975C GC-MS) was used to analyse the reaction liquid samples regularly. The test method was same as described in ref. 59 (link).
3
GC/MS Analysis of Urinary Steroids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC/MS analysis was performed on an Agilent 7890/5975C GC/MS (Agilent Technologies, Santa Clara, CA, USA). Steroids were separated using a 25 mm × 0.2 mm × 0.33 μm Ultra-1 column (J&W Scientific, Folsom, CA, USA). The sample injection volume was 2 μl with a split ratio of 5:1. Helium (99.9996%) was used as the carrier gas, and the flow rate was 1.0 ml/min. The oven temperature program was as follows: the initial temperature was 215°C, ramped to 260°C at 1°C/min and then ramped to 320°C at 15°C/min, and held for 5 min. The injector and transfer line temperatures were both 280°C. Full scan mode and selective ion scan were used for qualitative and quantitative analysis of urinary steroids, respectively. During the analysis process, different groups of samples were interspersed, and one QC sample was added to the analysis sequence for every five samples.
4
GC-MS Analysis of Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC–MS analysis was performed on an Agilent 7890/5975C GC–MS (Agilent Technologies, Santa Clara, CA, USA). FAs were separated using a 30 m × 0.25 mm × 0.25 μm DB-5 MS fused silica capillary column (J&W Scientific, Folsom, CA, USA). The sample injection volume was 1 μL with a split ratio of 10:1. Helium (99.9996%) was used as the carrier gas and the flow rate was 1.2 mL/min. The injector and transfer line temperatures were both 280°C. The oven temperature program was: 60°C for 1 min, then ramped to 200°C at 20°C/min, and held for 3 min; later, ramped to 280°C at 5°C/min, and held for 5 min. The metabolic data were collected at the mass scan range from 50 to 500 amu after a solvent delay of 3.5 min. All samples were analyzed in a random order.
In order to monitor the repeatability of sample analysis, quality control (QC) samples were added into the analysis sequence for every 3 samples. QC samples were prepared by equally mixing the tested serum samples, and processed together with samples.
In order to monitor the repeatability of sample analysis, quality control (QC) samples were added into the analysis sequence for every 3 samples. QC samples were prepared by equally mixing the tested serum samples, and processed together with samples.
5
GC-MS Metabolic Profiling of Plasma Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Metabolic profiling of plasma samples was acquired using an Agilent 7890/5975C GC‐MS (Agilent Technologies, USA). Each derivatized sample (1 μL) was injected into a DB‐5 fused silica capillary column (30 m × 0.25 mm × 0.25 μm; J&W Scientific, Folsom, CA, USA) with a split ratio of 10:1. The temperature program was as follows: the initial column temperature of 80°C was maintained for 5 minutes, then increased to 170°C at 5°C/min, followed by an increase to 300°C at 10°C/min, and maintained for 5 minutes. The injection temperature was 300°C. The temperatures of the inlet and ion source were set at 280 and 230°C, respectively. High‐purity helium (99.9996%) was used as the carrier gas, with a constant flow rate of 1.1 mL/min. The data were acquired in a full scan with 30‐600 m/z. Plasma samples were running at random and the QC sample was injected every 10 samples for evaluation.
Identification of metabolites in plasma was carried out by library search : NIST (http://www.nist.gov/srd ), Wiley (http://onlinelibrary.wiley.com/ ) and Fiehn (http://fiehnlab.ucdavis.edu/ ), retention index, and confirmation of authentic standard.
Identification of metabolites in plasma was carried out by library search : NIST (
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!