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1120 compact lc

Manufactured by Agilent Technologies
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Sourced in Germany, United Kingdom

The 1120 Compact LC is a liquid chromatography system designed for routine analysis in a laboratory setting. It provides a reliable and compact solution for separation and identification of chemical compounds. The device features automated sample handling and temperature control capabilities to ensure consistent and reproducible results.

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Lab products found in correlation

Silica gel 60, by Merck Group (2 mentions) Avance 400 mhz ft nmr, by Bruker (2 mentions) Eclipse plus c18 column, by Daicel (2 mentions) Chiralcel od h column, by Daicel (2 mentions) Jnm la 300, by Bruker (2 mentions) Hypersil gold, by Thermo Fisher Scientific (1 mentions) High performance liquid chromatography instrument, by Agilent Technologies (1 mentions) Zorbax eclipse plus c18 column, by Agilent Technologies (1 mentions)

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Agilent 1120 Compact LC (5 citations) 1120 Compact LC HPLC system (4 citations) 1120 Compact LC system (3 citations)

7 protocols using 1120 compact lc

1

Quantitative Analysis of Compounds by IR Spectroscopy and HPLC

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Mid-infrared (IR) spectroscopy was carried out by the Universal Attenuated Total Reflectance (UATR) spectrometer with the following instrument (PerkinElmer) specifications: spectrum version=10.03.09; model=spectrum 2; serial number=94133; number of scans=24; resolution=4. High-performance liquid chromatography (HPLC) was performed using the following instrument (Agilent) specification: pump=G4289A, detector=UV G1314, data software=chemstation enterprise view, column=COSMOSIL Cholester (4.6 x 250 mm) (Nacalai Tesque INC), chromatography interface=Agilent 1120 compact LC. The chromatographic conditions used included: column temperature=30°C; injection volume= 10 μl; flow rate= 1 ml/min; wavelength=254 nm; solvent system=acetonitrile: 0.1% V/V formic acid (100:0, 10 mins; 90:10, 5 mins; 80:20, 50 mins; 70:30, 5 mins; 60:40, 5 mins; 50:50, 5 mins).
Dofuor A.K., Djameh G.I., Ayertey F., Bolah P., Amoa-Bosompem M., Kyeremeh K., Okine L.K., Gwira T.M, & Ohashi M. (2019). Antitrypanosomal Effects of Zanthoxylum zanthoxyloides (Lam.) Zepern. & Timler Extracts on African Trypanosomes. Evidence-based Complementary and Alternative Medicine : eCAM, 2019, 1730452.
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2

Analytical Characterization of Organic Compounds

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All chemical reagents were commercially available. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC-MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Lee S., Kang D.W., Ryu H., Kim C., Ann J., Lee H., Kim E., Hong S., Choi S., Blumberg P.M., Frank-Foltyn R., Bahrenberg G., Stockhausen H., Christoph T, & Lee J. (2017). t-Butyl pyridine and phenyl C-region analogues of 2-(3-fluoro-4-methylsulfonylaminophenyl)propanamides as potent TRPV1 antagonists. Bioorganic & medicinal chemistry, 25(8), 2451-2462.
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3

Synthesis and Characterization of Novel Compounds

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All chemical reagents were commercially available. Melting points were determined on a Büchi Melting Point B-540 apparatus and are uncorrected. Silica gel column chromatography was performed on silica gel 60, 230–400 mesh, Merck. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra were recorded on JEOL JNM-LA 300 [300 MHz (1H), 75 MHz (13C)] and Bruker Avance 400 MHz FT-NMR [400 MHz (1H), 100 MHz (13C)] spectrometers. Chemical shifts are reported in ppm units with Me4Si as a reference standard. Mass spectra were recorded on a VG Trio-2 GC–MS and 6460 Triple Quad LC/MS. All final compounds were purified to >95% purity, as determined by high-performance liquid chromatography (HPLC). HPLC was performed on an Agilent 1120 Compact LC (G4288A) instrument using an Agilent Eclipse Plus C18 column (4.6 × 250 mm, 5 μm) and a Daicel Chiralcel OD-H column (4.6 × 250 mm, 5 μm).
Ann J., Ki Y., Yoon S., Kim M.S., Lee J.U., Kim C., Lee S., Jung A., Baek J., Hong S., Choi S., Pearce L.V., Esch T.E., Turcios N.A., Lewin N.E., Ogunjirin A.E., Herold B.K., McCall A.K., Blumberg P.M, & Lee J. (2016). 2-Sulfonamidopyridine C-region analogs of 2-(3-fluoro-4-methylsulfonamidophenyl)propanamides as potent TRPV1 antagonists. Bioorganic & medicinal chemistry, 24(6), 1231-1240.
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4

Losartan Liposome Encapsulation Optimization

Cited 1 time
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As per published reports, the amount of losartan entrapped in the formulation was optimized using the unfunctionalized formulation (Hamdan, 2015 ; Lakkadwala and Singh, 2019 (link); Singh et al., 2020 ). Liposomes were prepared with different loading concentrations of losartan potassium. Before and after purification of the liposome, the formulation samples were lysed using acetonitrile, centrifuged at 3000rpm for 10 minutes and the supernatant injected into the High-Performance liquid chromatograph (Agilent Technologies 1120 Compact LC) for losartan quantification. Per published reports with slight modifications, losartan was quantified using a high-performance liquid chromatography instrument (Agilent Santa Clara, CA, USA) (Furtek and Lo, 1992 ; Ritter et al., 1997 (link); Soldner et al., 1998 (link)). A mobile phase mixture consisting of 0.1M potassium phosphate buffer (pH 3) and acetonitrile (ratio 17:3) was run on a C-18 column (Thermo scientific Hypersil Gold, 5μm, 250 × 4.6 mm). The mobile phase was run at a flow rate of 0.9 mL/min, and losartan was detected at a wavelength of 254 nm.
The size and polydispersity index (PDI) were also determined for each loading concentration. The following formula was used to calculate Losartan potassium encapsulation efficiency ( LEE ) and losartan loading capacity ( LLC ).
Optiz T., Grooms S.Y., Bennett M.V, & Zukin R.S. (2000). Remodeling of α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor subunit composition in hippocampal neurons after global ischemia. Proceedings of the National Academy of Sciences of the United States of America, 97(24), 13360-13365.
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5

HPLC Analysis of Dye Degradation

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The dye and its degraded products were analyzed by HPLC system (Agilent 1120 Compact LC, Germany) with a Reverse Phase (RP) C18 column (4.6 X 250 mm, 5 micron).
The sample volume was 20μl and analyzed using a UV detector at 274 nm. The mobile phase was 100% methanol run in isocratic flow with a flow rate of 1 mL/min for 20 minutes to obtain chromatograms. The degradation was analyzed by comparing the peak area values.
Jayapal M., Jagadeesan H., Krishnasamy V., Shanmugam G., Muniyappan V., Chidambaram D, & Krishnamurthy S. (2022). Demonstration of a plant-microbe integrated system for treatment of real-time textile industry wastewater. Environmental pollution (Barking, Essex : 1987), 302.
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6

Quantification of Meropenem Uptake by Bacteria

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HPLC from Agilent Technologies (1120 Compact LC) with a C18 column as a stationary phase (at 30 0 C) was used to quantify the meropenem uptake by the bacteria. A mixture of 0.1% aqueous acetic acid (solution A) and methanol (solution B) was used as a mobile phase with a gradient program (Description in Table 2). The flow rate of the mobile phase was kept at 1 mL/ minute for chromatographic separation. The detection wavelength for meropenem was fixed at 300 nm. The elution of meropenem happened in between 13-14 minutes. The standard curve was formulated with known concentrations of meropenem (5, 25, 50, 75, and
Chowdhury A.R., Mukherjee D., Singh A.K, & Chakravortty D. (2022). Loss of outer membrane protein A (OmpA) impairs the survival of Salmonella Typhimurium by inducing membrane damage in the presence of ceftazidime and meropenem. The Journal of antimicrobial chemotherapy, 77(12).
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7

CNAD Release from Lactose-capped MSNPs

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To determine the amount of CNAD released from uncapped and lactose-capped MSNPs, 10 mg of loaded MSNPs were added to 5 ml PBS and incubated at 30°C and 200 rpm during 48h. A sample of 200 µl was analysed every hour via liquid chromatography (LC) using an Agilent Technologies 1120 Compact LC, equipped with a Zorbax Eclipse Plus C18 column (Agilent Technologies, UK). Samples were analysed using an isocratic ratio 80:20 (acetonitrile:H2O), UV detector (λ = 270 nm), flow of 1 ml/min and an injection volume of 10 µl over 4 minutes.
Bravo Cadena M., Preston G.M., Van der Hoorn R.A.L., Townley H.E, & Thompson I.P. (2018). Species-specific antimicrobial activity of essential oils and enhancement by encapsulation in mesoporous silica nanoparticles. Industrial Crops & Products., 122.
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