Eclipse widefield microscope

Manufactured by Nikon

The Eclipse widefield microscope is a high-performance optical instrument designed for laboratory and research applications. It features a widefield observation system that provides a large field of view, enabling efficient sample observation and analysis. The microscope is equipped with advanced optical components to deliver clear, high-resolution images for a variety of applications.

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Lab products found in correlation

Vectashield mounting medium, by Vector Laboratories (1 mentions) Halocarbon oil 27, by Merck Group (1 mentions) Trim scope 2, by Miltenyi Biotec (1 mentions) W plan apochromat 40x 1.4 oil immersion objective, by Olympus (1 mentions) E600 microscope, by Nikon (1 mentions)

Close spelling variants of this product

Eclipse Ti2 inverted widefield microscope Eclipse NiE Upright Widefield Microscope Nikon 90i Eclipse widefield microscope Widefield Ti-Eclipse inverted microscope Eclipse 90i widefield fluorescent microscope Eclipse Ti2 widefield microscope Eclipse Ti widefield microscope Nikon Ti Eclipse inverted widefield fluorescence microscope Nikon Ti2 Eclipse Color and Widefield Microscope Eclipse Ti-E widefield fluorescence microscope

5 protocols using eclipse widefield microscope

Dfos mRNA Localization in Drosophila Embryos

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Embryos were fixed with 3.7% formaldehyde/heptane for 20 min followed by methanol devitellinization for in situ hybridization and visualization of 3xmCherry. The Dfos cDNA clone SD04477 was obtained from the DGRC. T7 or T3 polymerase-synthesized digoxigenin-labelled anti-sense probe preparation and in situ hybridization was performed using standard methods (Lehmann & Tautz, 1994) (link). Images were taken with a Nikon-Eclipse Wide field microscope with a 20X 0.5 NA DIC water Immersion Objective. Embryos were mounted after immunolabeling in Vectashield Mounting Medium (Vector Labs, Burlingame, USA) and imaged with a Zeiss Inverted LSM700 and LSM800 Confocal Microscope using a Plain-Apochromat 20X/0.8 Air Objective or a Plain-Apochromat 63X/1.4 Oil Objective as required.

Belyaeva V., Wachner S., Gyoergy A., Emtenani S., Gridchyn I., Akhmanova M., Linder M., Roblek M., Sibilia M., & Siekhaus D.E. (2020). Cortical actin properties controlled by Drosophila Fos aid macrophage infiltration against surrounding tissue resistance.

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Multiphoton Imaging of Drosophila Embryogenesis

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Embryos were dechorionated in 50% bleach for 4 min, washed with water, and mounted in halocarbon oil 27 (Sigma) between a coverslip and an oxygen permeable membrane (YSI). The anterior dorsolateral region of the embryo was imaged on an inverted multiphoton microscope (TrimScope II, LaVision) equipped with a W Plan-Apochromat 40X/1.4 oil immersion objective (Olympus). GFP and mCherry were imaged at 860 nm and 1100 nm excitation wavelengths, respectively, using a Ti-Sapphire femtosecond laser system (Coherent Chameleon Ultra) combined with optical parametric oscillator technology (Coherent Chameleon Compact OPO). Excitation intensity profiles were adjusted to tissue penetration depth and Z-sectioning for imaging was set at 1 or 1.5 mm for tracking and segmentation respectively. For long-term imaging, movies were acquired for 180-200 minutes with a frame rate of 40 seconds. All embryos were imaged with a temperature control unit set to 28.5 C. To assess potential changes in germband extension and retraction in the egr 1 embryos, wild type and egr 1 embryos were collected for 30 minutes and then imaged for a further 10 hours using a Nikon-Eclipse Wide field microscope with a 20X 0.5 NA DIC water Immersion Objective. Bright field images were taken every 5 minutes.

Ratheesh A., Biebl J., Vesela J., Smutny M., Papusheva E., Krens S.F.G., Kaufmann W., Gyoergy A., Casano A.M, & Siekhaus D.E. (2018). Drosophila TNF Modulates Tissue Tension in the Embryo to Facilitate Macrophage Invasive Migration. Developmental cell, 45(3).

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Fluorescent and Confocal Microscopy Imaging

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Routine fluorescent and/or live images were taken using Nikon Eclipse widefield microscope. Confocal images were taken using a Leica TCS SPE laser scanning system. Following immunohistochemical staining or haematoxylin and eosin staining, a Nikon E600 microscope was used to take colour photographs at 20 × magnification.

Jones S., Ashworth J.C., Meakin M., Collier P., Probert C., Ritchie A.A., Merry C.L, & Grabowska A.M. (2023). Application of a 3D hydrogel-based model to replace use of animals for passaging patient-derived xenografts. In Vitro Models, 2(3-4), 99-111.

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DHA-Induced Senescence in A375P Cells

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A375P cells were plated in 35 mm fluorodishes (World Precision) at a density of 10,000 cells per dish. Cells were allowed to adhere overnight, and then dosed with fresh medium containing 5 mM DHA for 24, 48, and 72 h. Senescence was detected using Cellular Senescence Assay Kit (Biotium) following manufacturer’s instructions. Briefly, after the designated time points, untreated and treated cells were washed twice with PBS and fixed for 5 min. Cells were then washed three times in PBS and stained overnight with X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside). The staining solution was then removed and cells were washed a final time in PBS, then stored in 70% glycerol until imaged with a Nikon Eclipse wide field microscope. Three biological replicates were performed and images are representative.

Smith K.R., Granberry M., Tan M.C., Daniel C.L, & Gassman N.R. (2017). Dihydroxyacetone induces G2/M arrest and apoptotic cell death in A375P melanoma cells. Environmental toxicology, 33(3), 333-342.

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Quantitative Histological Analysis of Masson's Trichrome

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Bright-field photomicrographs of Masson’s Trichrome stained tissues were taken in high resolution from randomly selected areas without any selection using a Nikon Eclipse widefield microscope. The images were then quantified for blue trichrome staining using thresholding in ImageJ software (NIH).

Mukherjee A., Epperly M.W., Fisher R., Hou W., Shields D., Saiful Huq M., Pifer P.M., Mulherkar R., Wilhite T.J., Wang H., Wipf P, & Greenberger J.S. (2023). Inhibition of tyrosine kinase Fgr prevents radiation-induced pulmonary fibrosis (RIPF). Cell Death Discovery, 9, 252.

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