Anti mmp11

Manufactured by Cell Signaling Technology

Sourced in United Kingdom

Anti-MMP11 is a laboratory product from Cell Signaling Technology that detects the MMP11 protein. MMP11 is a member of the matrix metalloproteinase family and is involved in extracellular matrix remodeling.

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3 protocols using anti mmp11

Comprehensive Protein Expression Analysis

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The assays were performed as previously described 13 (link), using anti-Cdc42, anti-P21, anti-vWF, anti- VEGFC (Proteintech), anti-β-actin, anti-E-cadherin, anti-SIX1 anti-cyclinD1(Santa Cruz Biotechnology), anti-ERK, anti-p-ERK, anti-AKT, anti-p-AKT, anti- PLC-γ, anti-p-PLC-γ, anti-STAT3, anti-p-STAT3, anti- EGFR, anti-MMP-9, anti-MMP-11, and anti-PCNA (Cell Signaling Technology) antibodies.

Zhao Z., Li L., Du P., Ma L., Zhang W., Zheng L., Lan B., Zhang B., Ma F., Xu B., Zhan Q, & Song Y. (2019). Transcriptional Downregulation of miR-4306 serves as a New Therapeutic Target for Triple Negative Breast Cancer. Theranostics, 9(5), 1401-1416.

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Western Blot Analysis of Cellular Proteins

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Cells were lysed in RIPA buffer (Boston Bioproducts) supplemented with protease inhibitors (Complete, EDTA-free; Roche Diagnostics) and PMSF (Calbiochem). Lysates were separated on a 12% acrylamide gel and subjected to Western blot analysis. Immunoblots were incubated over night at 4°C with the following primary antibodies: anti-RBAK (mouse monoclonal; MBL; 1:2, 000), anti-MMP11 (Rabbit polyclonal; Cell Signaling Technologies; 1:1, 000), anti-pro-caspase 3, P17 (Proteintech, UK), and anti-Actin antibodies (goat polyclonal ; Santa Cruz Biotechnology; 1:2, 000). Goat anti-mouse IgG-HRP and goat anti-rabbit IgG-HRP (Sigma–Aldrich, USA) secondary antibodies were used to visualize bands using Amersham ECL Prime (GE Healthcare, UK). Signal intensity of Western blots was quantified by Quantity One Software (Bio-Rad, USA).

Wan X., Pu H., Huang W., Yang S., Zhang Y., Kong Z., Yang Z., Zhao P., Li A., Li T, & Li Y. (2016). Androgen-induced miR-135a acts as a tumor suppressor through downregulating RBAK and MMP11, and mediates resistance to androgen deprivation therapy. Oncotarget, 7(32), 51284-51300.

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Immunoprecipitation and Western Blot Analysis

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MCF7 cells were transfected as previously indicated and then lysed in lysis buffer. The samples were centrifuged at 4000 × g at 4°C for 10 min, and the supernatants were retained. Then, the supernatants were incubated with anti-MMP11 (1 µg/ml) or IgG (1 µg/ml; product no. 6990; Cell Signaling Technology, Inc.) at 4°C for 4 h and protein A/G-Sepharose beads (10 µl) for 2 h at 4°C. The immunocomplexes were washed three times, boiled in sample buffer, and subjected to western blotting with anti-Smad2 antibody.

Zhuang Y., Li X., Zhan P., Pi G, & Wen G. (2021). MMP11 promotes the proliferation and progression of breast cancer through stabilizing Smad2 protein. Oncology Reports, 45(4), 16.

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