Orca flash 4.0 lt ccd camera

Manufactured by Hamamatsu Photonics

The ORCA-flash 4.0 LT CCD camera is a scientific-grade camera designed for a wide range of imaging applications. It features a high-resolution 4.2-megapixel CCD sensor with a pixel size of 6.5 μm. The camera is capable of delivering fast frame rates and high quantum efficiency, making it suitable for a variety of low-light imaging tasks.

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Lab products found in correlation

Bx63 fluorescence microscope, by Hamamatsu Photonics (4 mentions) Cellsens software v 1.18, by Olympus (4 mentions) Bond rxm, by Leica (4 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (4 mentions) Ab5076, by Abcam (2 mentions) Ab32454, by Abcam (2 mentions)

4 protocols using orca flash 4.0 lt ccd camera

Immunohistochemical Detection of SARS-CoV-2

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Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Tris-buffered saline (TBS). Blocking was performed with 1% donkey serum diluted in TBS. Sections were stained for DAPI (Sigma) and mounted on glass coverslips in ProLong Gold Antifade mounting medium and stored at ambient temperature until imaging. Images were captured using an Olympus BX63 fluorescence microscope equipped with a motorized stage and Hamamatsu ORCA-flash 4.0 LT CCD camera. Images were collected and regions of interest quantified with Olympus cellSens software (v 1.18) using an Olympus X- line apochromat 10X (0.40 N.A.), 20X (0.8 N.A.) or 40X (0.95 N.A.) air objectives, or Uplan Flour X100 oil immersion (1.3 N.A.) objective.

Burke B., Rocha S.M., Zhan S., Eckley M., Reasoner C., Addetia A., Lewis J., Fagre A., Charley P.A., Richt J.A., Weiss S.R., Tjalkens R.B., Veesler D., Aboellail T, & Schountz T. (2023). Regulatory T cell-like response to SARS-CoV-2 in Jamaican fruit bats (Artibeus jamaicensis) transduced with human ACE2. PLOS Pathogens, 19(10), e1011728.

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Multimodal Imaging of SARS-CoV-2 Infection

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Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (CSU; 1:500), microtubule-associated protein 2 (Abcam; ab32454; 1:500), ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50)/glial fibrillary acidic protein (Sigma; 3893;1:500) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Tris-buffered saline (TBS). Blocking was performed with 1% donkey serum diluted in TBS. Sections were stained for DAPI (Sigma) and mounted on glass coverslips in ProLong Gold Antifade mounting medium and stored at ambient temperature until imaging. Images were captured using an Olympus BX63 fluorescence microscope equipped with a motorized stage and Hamamatsu ORCA-flash 4.0 LT CCD camera. Images were collected with Olympus Cellsens software (v 1.18) using an Olympus X-line apochromat 10X (0.40 N.A.), 20X (0.8 N.A.) or 40X (0.95 N.A.) air objectives, or Uplan Flour X100 oil immersion (1.3 N.A.) objective.

Fagre A., Lewis J., Eckley M., Zhan S., Rocha S.M., Sexton N.R., Burke B., Geiss B., Peersen O., Kading R., Rovnak J., Ebel G.D., Tjalkens R.B., Aboellail T, & Schountz T. (2020). SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for reverse zoonosis to New World rodents. bioRxiv.

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Immunostaining of SARS-CoV-2 in Brain Tissue

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Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500), microtubule-associated protein 2 (Abcam; ab32454; 1:500), ionized calcium binding adaptor molecule 1 (Abcam; ab5076; 1:50)/glial fibrillary acidic protein (Sigma; 3893;1:500) using a Leica Bond RXm automated staining instrument following permeabilization using 0.01% Triton X diluted in Tris-buffered saline (TBS). Blocking was performed with 1% donkey serum diluted in TBS. Sections were stained for DAPI (Sigma) and mounted on glass coverslips in ProLong Gold Antifade mounting medium and stored at ambient temperature until imaging. Images were captured using an Olympus BX63 fluorescence microscope equipped with a motorized stage and Hamamatsu ORCA-flash 4.0 LT CCD camera. Images were collected and regions of interest quantified with Olympus cellSens software (v 1.18) using an Olympus X-line apochromat 10X (0.40 N.A.), 20X (0.8 N.A.) or 40X (0.95 N.A.) air objectives, or Uplan Flour X100 oil immersion (1.3 N.A.) objective.

Fagre A., Lewis J., Eckley M., Zhan S., Rocha S.M., Sexton N.R., Burke B., Geiss B., Peersen O., Bass T., Kading R., Rovnak J., Ebel G.D., Tjalkens R.B., Aboellail T, & Schountz T. (2021). SARS-CoV-2 infection, neuropathogenesis and transmission among deer mice: Implications for spillback to New World rodents. PLoS Pathogens, 17(5), e1009585.

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Immunohistochemical Analysis of SARS-CoV-2 in Tissue

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Paraffin embedded tissue sections were stained for SARS-CoV-2 nucleocapsid protein (1:500) and ionized calcium binding adaptor molecule 1 (Abcam, ab5076; 1:50) using a Leica Bond RX^m automated staining instrument following permeabilization using 0.01% Triton X diluted in Tris-buffered saline (TBS). Blocking was performed with 1% donkey serum diluted in TBS. Sections were stained with DAPI (Sigma) and mounted on glass coverslips in ProLong Gold Antifade mounting medium and stored at ambient temperature until imaging. Images were captured using an Olympus BX63 fluorescence microscope equipped with a motorized stage and Hamamatsu ORCA-flash 4.0 LT CCD camera. Images were collected with Olympus cellSens software (v 1.18) using an Olympus X-line apochromat 10× (0.40 N.A.), 20× (0.8 N.A.) or 40× (0.95 N.A.) air objectives, or Uplan Fluor ×100 oil immersion (1.3 N.A.) objective. Regions of interest (ROIs) were drawn around the perimeter of each tissue section and intensity thresholds were kept constant across groups analyses were performed blinded. Co-localization and intensity measurements were obtained using the Count and Measure feature of cellSens.

Fagre A.C., Manhard J., Adams R., Eckley M., Zhan S., Lewis J., Rocha S.M., Woods C., Kuo K., Liao W., Li L., Corper A., Challa D., Mount E., Tumanut C., Tjalkens R.B., Aboellail T., Fan X, & Schountz T. (2020). A Potent SARS-CoV-2 Neutralizing Human Monoclonal Antibody That Reduces Viral Burden and Disease Severity in Syrian Hamsters. Frontiers in Immunology, 11, 614256.

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