When the plants were at a height of approximately 20 cm, 1 mL L-1 10% Basta solution (8.0 mg l-1) was used for preliminary screening. The plants were sprayed three times every 6 days. Genomic DNA was extracted from the alfalfa leaves using a Plant Genomic DNA extraction kit (Tiangen, Beijing). The PCR was conducted with a genomic DNA template in PCR premix using two convergent primers that were complementary to the CsALDH gene and another pair of primers that were complementary to the bar gene. DNA amplification was performed at 94°C for 3 min; 35 cycles of 94°C for 30 s, 50°C for 45 s, and 72°C for 1 min 30 s, and then a final extension at 72°C for 10 min. Total RNA was extracted using the UNIQ-10 column total RNA extraction kit (Sangon Biotech, Shanghai). The RT-PCR conditions were identical for both CsALDH and MsActin, as described in the genomic PCR analysis. The PCR products were separated on a 1.2% agarose gel, stained with GelStrain (TransGene Biotech, Beijing), and visualized under UV. Sixteen transgenic plants showing highly expressed CsALDH were selected for further stress tolerance and phenotype analysis. All subsequent experiments were conducted on cloned plants from the T0 generation of transgenic plants.
Uniq 10 column total rna extraction kit
Manufactured by Sangon
Sourced in China
The UNIQ-10 column total RNA extraction kit is a laboratory equipment designed for the isolation and purification of total RNA from various biological samples. The kit utilizes a silica-based column method to efficiently capture and purify RNA molecules, enabling users to obtain high-quality RNA for downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions)
Gelstrain, by Transgene (1 mentions)
Plant genomic dna extraction kit, by Tiangen Biotech (1 mentions)
Annexin 5 fluorescein isothiocyanate fitc apoptosis detection kit, by Thermo Fisher Scientific (1 mentions)
Sg fast qpcr master mix kit, by Sangon (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Genview (1 mentions)
Survivin sirna, by GenePharma (1 mentions)
Fam sirna, by GenePharma (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Sangon (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Solarbio (1 mentions)
Trizol reagent, by Solarbio (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
Scrambled sirna, by GenePharma (1 mentions)
6 protocols using uniq 10 column total rna extraction kit
1
Transformation and Screening of Alfalfa
2
Total RNA Extraction from IBC Tissue
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The UNIQ-10 column total RNA extraction kit (Sangon Biotech) was used to extract total RNA from the IBC tissue. An SMA4000 microscope (Merington Instruments) and a DYY-6C electrophoresis instrument (Beijing Liuyi) were used to observe the concentration and quality of the RNA.
3
Total RNA Extraction and Quantification
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Total RNA from IBC tissues and cultured cells was extracted using an UNIQ-10 column Total RNA Extraction Kit (Sangon Biotech). The RNA concentration and purity were assessed using a SMA4000 microspectrophotometer (Merinton Instrument, Inc.) and applying RNA electrophoresis by DYY-6C electrophoresis apparatus (Liuyi, Beijing).
4
Total RNA Extraction from BC Tissue
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Total RNA was extracted from BC tissue using a UNIQ-10 Column Total RNA Extraction Kit (Sangon Biotech, China). The concentration and purity of RNA were determined using a SMA4000 microspectrophotometer (Merinton Instrument, Inc.) and a DYY-6C electrophoresis apparatus (Liuyi, China).
5
Evaluating Peanut Oil's Protective Effects
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Peanut oil without antioxidant was supplied by Dehe Food Technology Co., Ltd. (Wuxi, China). Chicken legs (Tyson Foods Inc.) were purchased at a local supermarket. The HepG2 cell, an immortalized human hepatoma cell line, was bought from the Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences (SIBS) (Shanghai, China). Minimum essential medium (MEM), fetal bovine serum (FBS), trypsin and other cell culture materials were purchased from Gibco BRL, Life Technologies (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8), triglyceride (TG), malondialdehyde (MDA), catalase (CAT), superoxide dismutase (SOD) and total glutathione quantification (GSH) assay kits were all obtained from Beyotime Biotechnology Co., Ltd. (Shanghai, China). The reactive oxygen species (ROS) and bicinchoninic acid (BCA) protein assay kits were purchased from Thermo Fisher Scientific (Waltham, MA, USA). The annexin V-fluorescein isothiocyanate (FITC) apoptosis detection kit and cell cycle analysis kit were all purchased from Gibco BRL, Life Technologies (Carlsbad, CA, USA). UNIQ-10 column total RNA extraction kit, avian myeloblastosis virus reverse transcriptase kit and 2 × SG fast qPCR master mix kit were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). All chemicals and reagents were of analytical grade or higher.
6
Cyclo-(DP)3-Mediated Survivin Silencing
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Cyclo-(DP)3 was purchased from Shanghai Qiangyao Biological Technology (Shanghai, China). N, N-dimethylformamide (DMF), triethylamine (TEA), and CH3I were obtained from TCI (Shanghai) Development Co., Ltd. (Shanghai, China). Lipofectamine 2000 was provided by Thermo Fisher Scientific (Shanghai, China). Dimethylsulfoxide, hexamethylenediamine, and 2,2′-diamino-N-methyldiethylamine were purchased from Solarbio Life Sciences (Beijing, China). Survivin-siRNA, FAM-siRNA, and scrambled siRNA were purchased from GenePharma (Shanghai, China). Primers were purchased from Comate Bioscience (Changchun China). MTT was obtained from Genview (Beijing, China). DAPI, TRIzol reagent, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel preparation kits were acquired from Solarbio Life Sciences (Beijing, China). All-In-One RT MasterMix kits were purchased from TransGen Biotech (Beijing, China). The UNIQ-10 Column Total RNA Extraction Kit, Go Taq qPCR Master Mix Kit, and Annexin V-FITC/PI Apoptosis Detection Kit were purchased from Sangon Biotech (Shanghai,China). Anti-survivin antibody A5719 was obtained from Bimake (Houston, TX, USA). GAPDH (rabbit, AP0066) was obtained from Bioworld (Minneapolis, MN, USA).
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