This study was approved by the Institutional Review Board of Tokyo Medical University, and written informed consent was provided by all patients in accordance with the Declaration of Helsinki. Primary samples were obtained from the peripheral blood samples of CML patients after consent. Mononuclear cells were separated by Lymphosepar (Immuno-Biological Laboratories Co., Gunma, Japan) and cultured in RPMI1640 medium.
Lymphosepar
Manufactured by Immuno-Biological Laboratories
Sourced in Japan
Lymphosepar is a laboratory equipment designed for the separation and isolation of lymphocytes from whole blood samples. It is a specialized centrifugation device that utilizes density gradient media to effectively separate the lymphocyte fraction from other blood components. The core function of Lymphosepar is to provide a reliable and efficient method for isolating lymphocytes for further analysis and research.
Automatically generated - may contain errors
2 protocols using lymphosepar
1
CML Patient Blood Protocol
2
Culturing Myeloma and Endothelial Cells
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The human myeloma cell lines RPMI8226, MM1S, MM1R, and human umbilical vein endothelial cells (HUVECs) were purchased from ATCC. Primary myeloma cells were derived from the peripheral blood of two patients diagnosed with plasma cell leukemia (PCL). Mononuclear cells were separated using Lymphosepar (Immuno-biological Laboratories Co.). All cells were cultured in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum and 1% penicillin/streptomycin in a humidified incubator containing 5% CO2 at 37°C. The study protocol was approved by the Institutional Review Board of Tokyo Medical University (no. SH2408). Written informed consent was obtained from all patients in accordance with the tenets of the Declaration of Helsinki.
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