Sodium periodate naio4

Manufactured by Thermo Fisher Scientific

Sourced in United States

Sodium periodate (NaIO4) is an oxidizing agent commonly used in organic synthesis and analytical chemistry. It is a colorless crystalline solid that is soluble in water and other polar solvents. Sodium periodate is primarily used as a reagent for the cleavage of carbon-carbon bonds in carbohydrates and related compounds.

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Lab products found in correlation

Sodium phosphate dibasic anhydrous, by Thermo Fisher Scientific (2 mentions) Sodium phosphate monobasic monohydrate, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg h l alexa fluor 647, by Abcam (2 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide 98 mtt, by Thermo Fisher Scientific (2 mentions) Trichrome stain masson kit, by Merck Group (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Goat anti rabbit igg h l alexa fluor 488 ab150077, by Abcam (1 mentions) Polyfreeze, by Merck Group (1 mentions) Cd68 antibody ab125212, by Abcam (1 mentions) Weigert s iron hematoxylin solution, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (1 mentions) Bouin s solution, by Merck Group (1 mentions) Hrp conjugated anti rabbit igg antibody, by Cell Signaling Technology (1 mentions) Trap staining kit, by Fujifilm (1 mentions) Trametinib, by Selleck Chemicals (1 mentions) Hrp conjugated anti mouse igg antibody, by Cell Signaling Technology (1 mentions) Anti gapdh af1186, by Beyotime (1 mentions) Anti cd68 antibody ab125212, by Abcam (1 mentions) Bouin solution, by Merck Group (1 mentions)

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Sodium periodate (15 citations) NaIO4 (5 citations)

8 protocols using sodium periodate naio4

Pericardium Tissue Preparation and Modification

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Sodium
phosphate monobasic monohydrate, sodium
phosphate dibasic anhydrous, and sodium periodate (NaIO₄) were purchased from Acros Organics (Fair Lawn, New Jersey). Phosphate
buffer saline (PBS, 1×) was from Fisher Scientific Co. (Pittsburgh,
Pennsylvania). Fresh bovine pericardium was purchased from Sierra
for Medical Science (Whittier, California). Pericardium tissues were
cut into the desired dimension (2.5 cm × 2.5 cm), incubated in
buffer solution of a desired pH (10 mM sodium phosphate buffer adjusted
to a pH of 5.7, 6.7, 7.4, or 8.0) for 2 days and kept frozen until
testing.^{21 (link)} pH treatment had no impact on
the tensile modulus of the tissue substrate (Table
S1). PEG-ND²⁹ and PEG-D^{21 (link)} were prepared using a previously published method
using 4-armed 10k Da N-hydroxysuccinimide ester activated
poly(ethylene glycol) purchased from JenKem USA, Inc. (Allen, Texas).

Cencer M., Murley M., Liu Y, & Lee B.P. (2014). Effect of Nitro-Functionalization on the Cross-Linking and Bioadhesion of Biomimetic Adhesive Moiety. Biomacromolecules, 16(1), 404-410.

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Synthesis and Characterization of PEG-DA Hydrogel

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Sodium periodate (NaIO4, > 99.8%) was obtained from Acros Organics (Fair Lawn, NJ). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA). Phosphate buffer saline (PBS), tetraethyl orthosilicate (TEOS, 99.8%), ethanol (200 proof), sodium hydroxide, and acetic acid (Glacier) were purchased from Fisher Scientific Co. (Pittsburgh, PA). Histology mounting medium Polyfreeze, Trichrome Stain (Masson) Kit, Bouin’s solution, and Weigert’s iron hematoxylin solution were purchased from Sigma-Aldrich (St. Louis, MO). Anti-CD163 antibody (ab87099), goat anti-rabbit IgG H&L (Alexa Fluor 488) (ab150077), anti-CD68 antibody (ab125212), and goat anti-rabbit IgG H&L (Alexa Fluor 647) were purchased from Abcam (Cambridge, MA). 4,6-Diamidino-2-phenylindole (DAPI) was obtained from Invitrogen (Grand Island, NY). PEG-DA (Figure S1) was prepared using an 8-armed PEG (MW = 20 kDa, JenKem USA, TX) while following previously published protocols (22 (link), 30 (link)).

Pinnaratip R., Meng H., Rajachar R.M, & Lee B.P. (2018). Effect of Incorporating Clustered Silica Nanoparticles on the Performance and Biocompatibility of Catechol-Containing PEG-Based Bioadhesive. Biomedical materials (Bristol, England), 13(2), 025003.

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Chitosan-Based Molecular Crosslinking

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Chitosan, 3,4-dihydroxy hydrocinnamic acid, 1-ethyl-3-(3-dimethylamino-propyl)-carbodiimide hydrochloride (EDC), and dextran were purchased from Aladdin Reagents. Sodium periodate (NaIO₄) was purchased from Acros. Trametinib was purchased from Selleck. The TRAP staining kit was purchased from Wako. The RNA extraction kit was purchased from Chengdu Forge Biotechnology Co., Ltd. (China). HiFiScript gDNA Removal RT MasterMix and MagicSYBR Mixture were purchased from Beijing CWBIO Biotechnology Co., Ltd. (China). The BCA protein quantification kit and ultrasensitive ECL developer were purchased from Suzhou New Semi Biotechnology Co., Ltd. (China). Anti-phospho-p44/42 MAPK (ERK1/2) antibody (No. 4370) and anti-p44/42 MAPK (ERK1/2) antibody (No. 4695) were purchased from Cell Signaling Technology. Anti-GAPDH (AF1186) were purchased from Shanghai Beyotime Biotechnology Co., Ltd. (China). Anti-mouse IgG HRP-conjugated antibody and anti-rabbit IgG HRP-conjugated antibody were purchased from Cell Signaling Technology. Biotin-labeled goat anti-mouse/rabbit IgG polymer and DAB color development kits were purchased from Beijing Zhongshan Jinqiao Biotechnology Co., Ltd. (China).

Yu H., Wu Z., Bao X., Tang X., Zhang J., Zhang Y, & Hu M. (2022). A sustained-release Trametinib bio-multifunction hydrogel inhibits orthodontically induced inflammatory root resorption. RSC Advances, 12(26), 16444-16453.

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Biomaterial Characterization and Evaluation

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Sodium periodate (NaIO₄, >99.8%)
was obtained from Acros Organics (Fair Lawn, NJ). Bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 98% (MTT)
was from Alfa Aesar (Ward Hill, MA). 1X phosphate buffer saline was
from Fisher Scientific Co. (Pittsburgh, PA). Histology mounting medium
polyfreeze, Trichrome Stain (Masson) Kit, bouin solution, and Weiger’s
iron hematoxylin solution were purchased from Sigma-Aldrich (St. Louis,
MO). Anti-S100A4 antibody (ab27957), goat antirabbit IgG H&L (Alexa
Fluor 488) (ab150077), anti-CD68 antibody (ab125212), and goat antirabbit
IgG H&L (Alexa Fluor 647) were purchased from Abcam (Cambridge,
MA). 4′,6-Diamidino-2-phenylindole (DAPI) was obtained from
Invitrogen (Grand Island, NY). Laponite XLG (Laponite) was a gift
from Southern Clay Products, Inc. (Austin, TX). PEG-D4 was prepared
as previously described.³⁶

Liu Y., Meng H., Konst S., Sarmiento R., Rajachar R, & Lee B.P. (2014). Injectable Dopamine-Modified Poly(ethylene glycol) Nanocomposite Hydrogel with Enhanced Adhesive Property and Bioactivity. ACS Applied Materials & Interfaces, 6(19), 16982-16992.

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Dopamine-PEG Conjugation for Tissue Engineering

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Dopamine
HCl and N-methylmorpholine
were purchased from Acros Organics (Fair Lawn, New Jersey). The 4-arm
10k Da N-hydroxysuccinimide ester activated poly(ethylene
glycol) (PEG-NHS) was purchased from JenKem U.S.A., Inc. (Allen, TX).
Anhydrous dimethylformamide, sodium phosphate monobasic monohydrate,
sodium phosphate dibasic anhydrous, concentrated hydrochloric acid
(36.5–38%), and sodium periodate (NaIO₄) were purchased
from Acros Organics (Fair Lawn, New Jersey). Fresh bovine pericardium
was purchased from Sierra for Medical Science (Whittier, California).
Spectra/Por dialysis membrane (molecular weight cut off (MWCO): 3500
Da) was purchased from Spectrum Laboratories (Rancho Dominguez, California).
Dulbecco’s modified Eagle’s medium (DMEM; with 4.5 g/L
glucose and glutamine, without sodium pyruvate) and trypsin EDTA (0.05%
trypsin/0.53 mM EDTA in HBSS) were obtained from Corning Cellgro (Manassas,
VA). Fetal bovine serum and penicillin-streptomycin (10 units/mL)
were purchase from Thermo Scientific (Rockford, IL). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium
bromide 98% (MTT) was purchased from Alfa Aesar (Ward Hill, MA).

Cencer M., Liu Y., Winter A., Murley M., Meng H, & Lee B.P. (2014). Effect of pH on the Rate of Curing and Bioadhesive Properties of Dopamine Functionalized Poly(ethylene glycol) Hydrogels. Biomacromolecules, 15(8), 2861-2869.

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Multifunctional Wound Dressing Fabrication

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Chitosan (degree of deacetylation≈95%, 100–200 mps), dextran (Mw: ~70 kDa), 3, 4-dihydroxy hydrocinnamic acid, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), lead-oxide (ZnO) were purchased from Aladdin. Sodium periodate (NaIO₄) was purchased from Acros. 4-(Methylamino) Pyridine was purchased from TCI. Fibrin glue was purchased from Shanghai yuanye Bio-Technology Co., Ltd. Gelatin type A (from porcine skin, 300 Bloom), Live/Dead Cell Double Staining Kit, CORM-3, and GSNO were purchased from Sigma. CCK-8 was purchased from Beyotime. CD31, IL-6, TNF-a, IL-1b, and all other antibodies are purchased from Abcam. IR@dye 680-NHS ester and IR@dye 800-NHS ester were purchased from LI-COR.

Tang X., Ren J., Wei X., Wang T., Li H., Sun Y., Liu Y., Chi M., Zhu S., Lu L., Zhang J, & Yang B. (2023). Exploiting synergistic effect of CO/NO gases for soft tissue transplantation using a hydrogel patch. Nature Communications, 14, 2417.

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Hydrogel Synthesis Reagents Protocol

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N-isopropylacrylamide (NIPAM) (purity 98%) monomer was purchased from TCI (Tokyo, Japan). Acrylamide (AM) (purity > 99.5%) monomer was purchased from BioShop (Burlington, ON, Canada). Ammonium persulfate (APS) with a purity over 99% and N, N, N’, N’-tetramethylenediamine (TEMED) with a purity higher than 98% were purchased from Bio-Rad (Hercules, CA, USA). N, N’-methylenebis(acrylamide) (MBA) was obtained from Sigma Aldrich (St. Louis, MO, USA). Sodium periodate (NaIO₄) (purity 98%) was purchased from Alfa Aesar (Ward Hill, MA, USA).

Hu Y., Shin Y., Park S., Jeong J.P., Kim Y, & Jung S. (2022). Multifunctional Oxidized Succinoglycan/Poly(N-isopropylacrylamide-co-acrylamide) Hydrogels for Drug Delivery. Polymers, 15(1), 122.

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Oxidized Hyaluronan and Carboxymethyl Chitosan

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Carboxymethyl chitosan (CMCh) (degree of deacetylation 85–90%, viscosity = 5–300 mPas, Chitoscience, Halle (Saale, Germany), sodium hyaluronan (HA) (~1.5–1.8 × 10⁶ Da, Sigma-Aldrich, St. Louis, MO, USA), sodium periodate (NaIO₄) (Alfa Aesar, Haverhill, MA, USA), ethylene glycol (Sigma), hydroxylamine (Sigma-Aldrich), iron chloride (III) (Sigma-Aldrich), and phosphate buffered saline solution (PBS) 10 mM pH 7.4 (Gibco, Thermo Fisher, Waltham, MA, USA) were used as received. Sodium hyaluronan of low molecular weight (M_w ~200 kDa, Bioiberica, Barcelona, Spain) was oxidized (HAox) prior to use, as reported elsewhere [62 (link)], with a final oxidation degree of 48 ± 3.2% [67 (link),68 (link)].

Puertas-Bartolomé M., Włodarczyk-Biegun M.K., del Campo A., Vázquez-Lasa B, & San Román J. (2020). 3D Printing of a Reactive Hydrogel Bio-Ink Using a Static Mixing Tool. Polymers, 12(9), 1986.

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