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Quantikine rat il 6 and tnf α immunoassay kit

Manufactured by R&D Systems
Sourced in United States

The Quantikine Rat IL-6 and TNF-α Immunoassay Kit is a solid-phase ELISA designed to measure rat interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) levels in cell culture supernates, serum, and plasma. The kit utilizes the quantitative sandwich enzyme immunoassay technique.

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2 protocols using quantikine rat il 6 and tnf α immunoassay kit

1

Intestinal Injury Biomarker Quantification

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Plasma intestinal fatty acid binding protein (I-FABP), IL-6, and TNF-α levels were measured using commercially available enzyme immunoassay kits from TSZ ELISA (TSZ Scientific, Framingham, MA, USA) and Quantikine Rat IL-6 and TNF-α immunoassay kit (R&D Systems, Minneapolis, MN, USA). Plasma D-lactate levels were measured using commercially available colorimetric assay kit from Sigma-Aldrich Inc. following manufacturer's instructions. Absorbances were measured at 450 nm.
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2

Compound-Induced Cytokine Quantification

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Compound 48/80 (C48/80), Evans blue dye, toluidine blue dye, disodium chromoglycate (DSCG), formamide, formalin, alcohol, and sodium chloride were purchased from Sigma-Aldrich, Inc. St. Louis MO, USA. Quantikine rat IL-6 and TNF-α immunoassay kit procured from R&D Systems, Inc., Minneapolis, USA.
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