Tryptone

Manufactured by Teknova

Sourced in United States

Tryptone is a complex mixture of peptides and amino acids derived from the enzymatic digestion of casein. It serves as a nutrient source for the growth and cultivation of various microorganisms in microbiology and cell culture applications.

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Lab products found in correlation

Yeast extract, by Teknova (1 mentions) Yeast extract, by Hardy Diagnostics (1 mentions) X gal, by GoldBio (1 mentions) Vhx 1000 microscope, by Keyence (1 mentions)

3 protocols using tryptone

Chronic Wound Biofilm in db/db Mice

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Chronic wounds were made in db/db^−/− mice as previously described [24 (link)]. Six to seven-month-old db/db^−/− mice were treated intraperitoneally with a catalase inhibitor, 3-amino-1,2,4-triazole, at 1 g/kg body weight before wounding. Immediately after wounding, they were treated once topically with the glutathione peroxidase inhibitor, mercaptosuccinic acid, at 150 mg/kg body weight. Using our procedure, the wounds are fully chronic in 20 days and contain abundant biofilm. All bacteria forming the biofilm are from the mouse natural skin with no additional manipulation or introduction of laboratory-carried strains; therefore, the biofilm forms naturally from the microbiome in the skin. For these studies, the biofilms were collected using the Levine method with sterile cotton swabs and stored for bacteria identification both dry at -80°C and in Luria-Bertani (LB) media (10 g tryptone, 5 g yeast extract, and 10 g NaCl and Milli-Q water to 1 L; Teknova) supplemented with 20% glycerol. Swabs in LB media with 20% glycerol were then cultured in fresh LB media overnight at 37°C (150 rpm) and aliquots stored at -80°C.

Li X., Kim J., Wu J., Ahamed A.I., Wang Y, & Martins-Green M. (2020). N-Acetyl-cysteine and Mechanisms Involved in Resolution of Chronic Wound Biofilm. Journal of Diabetes Research, 2020, 9589507.

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Routine Bacterial Strain Culture

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Bacterial strains were grown aerobically at 37°C overnight in lysogeny broth (LB) (1% w/v tryptone (Teknova, Hollister, CA, USA), 0.5% w/v yeast extract (Hardy Diagnostics, Santa Maria, CA, USA), 1% w/v NaCl (VWR Life Sciences, Radnor, PA, USA)) with constant shaking or on LB agar (1.5% w/v agar; Genesee Scientific, San Diego, CA, USA) standing at 37°C. LB‐X‐gal was made by mixing in 40 μg/mL of X‐gal (GoldBio, St. Louis, MO, USA) to LB agar while it is liquid.

Zhang C., Datta S., Ratcliff W.C, & Hammer B.K. (2024). Constitutive expression of the Type VI Secretion System carries no measurable fitness cost in Vibrio cholerae. Ecology and Evolution, 14(3), e11081.

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Assessing Biofilm Formation Dynamics

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Liquid agar was prepared by autoclaving 1% tryptone (Teknova), 1% agar (Teknova) mixture. The liquid agar was cooled to 60˚C and Congo Red (EMD; final concentration 40 μg/mL) and Coomassie Blue (EMD; final concentration: 20 μg/mL) were added. TLCA stock solution, dissolved in MeOH, was vortexed for 2–3 min until clear. TLCA was added to liquid agar, different amounts were added to reach the final concentrations of 100 μM, 250 μM, and 1 mM TLCA. 60 mL of liquid agar mixture was poured into square plates (LDP, 10 cm x 10 cm x 1.5 cm) and left to solidify for ~18–24 h. Precultures of P. aeruginosa PA14 were grown in LB for 12–16 h at 37˚C while shaking at 250 rpm. Subcultures were prepared as 1:100 dilutions of precultures into fresh LB media and shaking for 2.5 h at 37˚C, at which point all subcultures had reached mid-exponential phase (optical density of ~0.4–0.6 at 500 nm). Morphology plates were dried for 20–30 min and 10 μL spots of subculture were spotted onto a morphology plate, with not more than four colonies per plate. Colony biofilms were grown at 25˚C and high humidity (+90%) for up to 5 d. Images were taken every 24 h with a Keyence VHX-1000 microscope.

Condren A.R., Kahl L.J., Boelter G., Kritikos G., Banzhaf M., Dietrich L.E, & Sanchez L.M. (2020). Biofilm inhibitor taurolithocholic acid alters colony morphology, specialized metabolism, and virulence of Pseudomonas aeruginosa. ACS infectious diseases, 6(4), 603-612.

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