Hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), and alkaline phosphatase (ALP) enzyme activity kits were obtained from Jiancheng (Nanjing, China). MitoTracker Red CMXRos (M7512) and Alexa Fluor 488 Phalloidin (A12379) were obtained from Invitrogen (Carlsbad, CA, USA). The XF Cell Energy Phenotype Test Kit, XFp extracellular flux analyzer, XFp culture microplates, and bicarbonate-free DMEM were obtained from Seahorse Bioscience Agilent Technologies (North Billerica, MA, USA). Hoechst 33342 (B2261) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP, C2759) were purchased from Sigma–Aldrich (St Louis, MO, USA). The following antibodies were used: CD90 (FITC, mouse anti-human, BioLegend, 328107), CD105 (PerCP/Cy5.5, mouse anti-human, BioLegend, 323215), CD19 (PE, mouse anti-human, BioLegend, 982402), CD34 (PE, mouse anti-human, BioLegend, 343505), AMPK (Abcam, ab80039), p-AMPK (Abcam, ab133448), anti-GAPDH (Abcam, ab9485,ab8245), goat anti-rabbit IgG (H + L)-HRP (Bio-Rad Laboratories, 1706515), goat anti-mouse IgG (H + L)-HRP (Bio-Rad Laboratories,1706516) and goat anti-rabbit IgG (H+L) Alexa Fluor 594-conjugated secondary antibody (Invitrogen, R37117).
Enzyme activity kit
Manufactured by Nanjing Jiancheng
Sourced in China
Enzyme activity kits are laboratory tools used to measure the catalytic activity of enzymes. They provide a standardized and quantitative method for determining the functional capacity of specific enzymes in various samples.
Automatically generated - may contain errors
Lab products found in correlation
Hoechst 33342 b2261, by Merck Group (1 mentions)
Percp cy5, by BioLegend (1 mentions)
Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions)
Goat anti mouse igg h l hrp, by Bio-Rad (1 mentions)
P ampk, by Abcam (1 mentions)
Goat anti rabbit igg h l hrp, by Bio-Rad (1 mentions)
Cd105, by BioLegend (1 mentions)
Mitotracker red cmxros, by Thermo Fisher Scientific (1 mentions)
Xfp extracellular flux analyzer, by Agilent Technologies (1 mentions)
Anti gapdh, by Abcam (1 mentions)
Xf cell energy phenotype test kit, by Agilent Technologies (1 mentions)
Bicarbonate free dmem, by Agilent Technologies (1 mentions)
5 protocols using enzyme activity kit
1
Metabolic Profiling of Stem Cells
2
Enzymatic Activities in Largemouth Bass
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The activities of superoxide dismutase (SOD), alkaline phosphatase (AKP), acid phosphatase (ACP), and lysozyme (LZM) in the serum and intestinal mucosa of the largemouth bass were determined according to the instructions of the enzyme activity kits (Jiancheng, Nanjing, China). The intestinal mucosa was collected according to a previously described method [35 (link)]. The intestinal mucosa was weighed and diluted with sterile PBS to a 1% tissue homogenate concentration and centrifuged at 4 °C, 5000× g, for 20 min. The obtained supernatant was used for subsequent enzyme activity determination. Based on the Bradford method, bovine serum albumin (BSA) was used as the standard to determine the total intestinal protein content [36 (link)]. Each group of samples was repeated three times.
3
Biochemical Profiles in Plant Stress Response
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Proline, MDA, and soluble sugar contents were measured as described by Cai et al. [71 ]. POD (Peroxidase), CAT (Catalase), SOD (Superoxide Dismutase), PPO (Polyphenol Oxidase), PAL (Phenylalanineammonialyase), and C4H (Cinnamate 4- Hydroxylase) activities were determined using enzyme activity kits (Jiancheng, Nanjing, China), in accordance with the instructions of the manufacturer. Endogenous JA content was determined by Nanjing Jisihuiyuan Biotechnology Co., Ltd. (Nanjing, China).
4
Antioxidant Enzyme Assessment in Liver Samples
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The liver samples were added with normal saline according to the ratio of weight liver: volume of normal saline (1:9), and the liver samples were fully broken under the condition of ice water bath. After the broken liver samples were centrifuged for 10 min (2500 r/min, 4 °C), the supernatant was taken after centrifugation for enzyme activity detection. The activity of peroxidase (POD) was determined by A084-2 colorimetric method, the activity of Lysozyme (LZM) was determined by A050 turbidimetric method, and the activity of superoxide dismutase was determined by superoxide dismutase. SOD activity was determined by A001-1 hydroxylamine method, catalase (CAT) activity was determined by A007-1 visible light method, total antioxidant capacity, T-AOC capacity was determined by A015 colorimetric method. Content of malondialdehyde (MDA) and activity of glutathione peroxidase (GSH-Px) were determined by A003-1 TBA method. The enzyme activity kit was purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), and the measurement procedure, principle and calculation formula were referred to the kit manual.
5
Enzyme Activity Assay in Cotton Leaves
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Cotton leaves were frozen in liquid nitrogen and quickly ground into a ne powder. Samples of 100 mg were homogenized in extraction buffer and the mixture was collected in a centrifuge tube. After centrifugation at 4°C and 14000×g for 10 min, the supernatant was the crude enzyme solution. The activities of CAT, POD, PAL and PPO were determined according to the guidelines of the enzyme activity kit (Nanjing Jiancheng Bioengineering Institute, China).
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