The rice bran-extracted samples thermally treated were analyzed for the residual GF2, GF3, and GF4 contents using an ultra-performance liquid chromatography system equipped with an ESI (Waters Corporation, Milford, MA, USA) coupled to an MS/MS system (Xevo TQS Micro, (Waters Corporation, Milford, MA, USA). The chromatographic separation was performed on a Luna amino—NH2 column (150 mm × 2 mm × 5 µm) (Phenomenex, Torrance, Los Angeles, CA, USA) [30 (link)]. The elution was performed at a constant flow rate of 0.45 mL/min following the program presented in Table 1 , with an injection volume of 10 µL and an overall run time of 5 min per sample injection. The Xevo TQS Micro MS/MS system runs in negative ionization mode. The initial optimization parameters are as follows: ionization potential (capillary) of 2.5 kV, source temperature of 150 °C, desolvation temperature of 500 °C, and nitrogen gas rate of 800 mL/h. The fragmentation conditions for measurement of GF2, GF3, and GF4 are also reported in Table 1 .
Luna amino nh2 column
Manufactured by Phenomenex
Sourced in United States
The Luna Amino (NH2) column is a high-performance liquid chromatography (HPLC) column designed for the separation and analysis of various analytes. It features a silica-based stationary phase bonded with amino (NH2) functional groups. The column is suitable for a range of applications, including the analysis of polar and hydrophilic compounds.
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Lab products found in correlation
3 protocols using luna amino nh2 column
1
Quantification of Glycosidic Flavonoids in Rice Bran
2
Aqueous Chromatographic Separation of Metabolites
3
Aqueous Chromatographic Separation of Metabolites
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Briefly, aqueous phase chromatographic separation was achieved using
three solvents: water (solvent A), water with 5mM ammonium acetate (pH 9.9),
and100% acetonitrile (ACN) (solvent B). The binary pump flow rate was 0.2
ml/min with a gradient spanning 80% B to 2% B over a 20 minute period
followed by 2% B to 80% B for a 5 min period and followed by 80% B for 13
minute time period. The flow rate was gradually increased during the
separation from 0.2 mL/min (0–20 mins), 0.3 mL/min (20–25
min), 0.35 mL/min (25–30 min), 0.4 mL/min (30–37.99 min), and
finally set at 0.2 mL/min (5 min). Glycolytic and TCA intermediates were
separated on a Luna Amino (NH2) column (3 µm, 100A 2
× 150 mm, Phenomenex), that was maintained in a
temperature-controlled chamber (37°C).
three solvents: water (solvent A), water with 5mM ammonium acetate (pH 9.9),
and100% acetonitrile (ACN) (solvent B). The binary pump flow rate was 0.2
ml/min with a gradient spanning 80% B to 2% B over a 20 minute period
followed by 2% B to 80% B for a 5 min period and followed by 80% B for 13
minute time period. The flow rate was gradually increased during the
separation from 0.2 mL/min (0–20 mins), 0.3 mL/min (20–25
min), 0.35 mL/min (25–30 min), 0.4 mL/min (30–37.99 min), and
finally set at 0.2 mL/min (5 min). Glycolytic and TCA intermediates were
separated on a Luna Amino (NH2) column (3 µm, 100A 2
× 150 mm, Phenomenex), that was maintained in a
temperature-controlled chamber (37°C).
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