Rpmi 1640 medium

Manufactured by Meilun

Sourced in China, United States

RPMI 1640 medium is a commonly used cell culture medium designed for the growth and maintenance of a variety of cell types, including human and animal cells. It provides a balanced salt solution and essential nutrients required for cell proliferation and survival.

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Lab products found in correlation

Fetal bovine serum (fbs), by Meilun (7 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (6 mentions) Dulbecco modified eagle medium (dmem), by Meilun (4 mentions) Mda mb 231, by Meilun (2 mentions) Hct116, by Meilun (2 mentions) Sw620, by American Type Culture Collection (2 mentions) Microplate reader, by Thermo Fisher Scientific (2 mentions) Rg7388, by Selleck Chemicals (1 mentions) Penicillin, by Meilun (1 mentions) Streptomycin, by Meilun (1 mentions) Penicillin streptomycin, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions) Penicillin streptomycin, by Meilun (1 mentions) Annexin 5 fitc apoptosis detection kit, by Meilun (1 mentions) Cell cycle and apoptosis analysis kit, by Meilun (1 mentions) Dcfh da, by Meilun (1 mentions) Hsp70, by Beyotime (1 mentions) Gpx4 rabbit polyclonal antibody, by Beyotime (1 mentions) Cell counting kit 8 cck 8, by Meilun (1 mentions) Bcl 2, by Wuhan Servicebio Technology (1 mentions) Tnf α, by Wuhan Servicebio Technology (1 mentions)

Close spelling variants of this product

RPMI-1640 (19 citations) 1640 medium (5 citations) RPMI-1640 complete medium (2 citations) RPMI 1640 cell culture medium (2 citations)

33 protocols using rpmi 1640 medium

Culturing and Treating Human Cancer Cell Lines

Cited 2 times

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Human tumour cell lines 22Rv1, RT4, DU145 and T24 were cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Meilunbio, Dalian, China) supplemented with 10% fetal bovine serum (FBS) (Thermo Fisher Scientific, MA, USA) and a cocktail of antibiotics (Beyotime Biotechnology, Shanghai, China). MCF7, T47D, Huh7 and HCT116 were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Meilunbio, Dalian, China) containing 10% FBS, 100 U/mL penicillin and 100 mg/mL streptomycin. All cells were purchased from ATCC and cultured in a humidified incubator at 37 °C with 5% CO2.
The MDM2 inhibitor XR-2 was designed and synthesized by Wu et al. [22 (link)]. The NMD inhibitor was synthesized according to the structure of the previous study [25 (link)]. RG7388 was purchased from Selleck (Selleck Chemicals, TX, USA). All compounds were dissolved in dimethyl sulfoxide (DMSO) and stored at −20 °C until further use.

Li Y., Wu M., Zhang L., Wan L., Li H., Zhang L., Sun G., Huang W., Zhang J., Su F., Tang M, & Xiao F. (2022). Nonsense-mediated mRNA decay inhibition synergizes with MDM2 inhibition to suppress TP53 wild-type cancer cells in p53 isoform-dependent manner. Cell Death Discovery, 8, 402.

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Culturing Human Breast Cancer Cell Lines

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The MCF10A (cat. no. CL-0525) cell line was purchased from Wuhan Procell Company, derived from human normal mammary epithelial cells, and was cultured in specialized MCF10A medium (Procell Life Science & Technology Co., Ltd.). The human BC cell lines, SKBR3 (cat. no. 1101HUM-PUMC000085), MCF7 (cat. no. 1101HUM-PUMC000013) and Hs578T (cat. no. 1101HUM-PUMC000670), were cultured in DMEM (Dalian Meilun Biology Technology Co.) supplemented with 10% fetal bovine serum (Shanghai ExCell Biological Products Co., Ltd.), 100 U/ml penicillin and 100 µg/ml streptomycin (Dalian Meilun Biology Technology Co., Ltd.) in a 5% CO₂ incubator at 37°C, while the MDA-MB-231 (cat. no. 1101HUM-PUMC000014) and MDA-MB-468 cells (cat. no. 1101HUM-PUMC000249) were cultured in RPMI-1640 medium (Dalian Meilun Biology Technology Co.). All the human BC cell lines were purchased from the ChineseNational Infrastructure of Cell line Resource (NICR).

Hu H., Wang Z.W., Hu S., Xiang Y., Deng Y., Wan F.J., Zhang T.C., Yang Z.Y, & Liao X.H. (2023). GNPNAT1 promotes the stemness of breast cancer and serves as a potential prognostic biomarker. Oncology Reports, 50(2), 157.

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Human Leukemia Cell Culture

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Cell culture of human leukemia cells HL-60, Kasumi-1 cell line was purchased from BNCC (Henan, China) and maintained according to the manufacturer's instructions. We have confirmed the cell lines used in the experiments by specialized STR analysis and tested them for mycoplasma contamination. Cells were grown in RPMI1640 medium (meilunbio, Dalian, China) containing 10% fetal bovine serum (FBS, meilunbio, Dalian, China).

Gang D., Jiang Y., Wang X., Zhou J., Zhang X., He X., Dong R., Huang Z, & Jiang S. (2023). Aging-related genes related to the prognosis and the immune microenvironment of acute myeloid leukemia. Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico, 25(10).

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Cell Culture Conditions for Cell Lines

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The Type Culture Collection of China Centre provided all cells that were maintained under 5% CO₂ at 37°C. HEK293T and ES2 cells were cultured in DMEM (Meilunbio, China) plus 10% fetal bovine serum (FBS, Gibco). Normal ovarian cells (IOSE), SKOV3, OVCAR3 and THP-1 cells were maintained in RPMI 1640 medium (Meilunbio, China) containing 10% FBS. All cells were authenticated by short tandem repeat.

Sheng B., Zhao B., Dong Y., Zhang J., Wu S., Ji H, & Zhu X. (2023). Copine 1 predicts poor clinical outcomes by promoting M2 macrophage activation in ovarian cancer. Carcinogenesis, 44(10-11), 748-759.

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Cell culture conditions for cancer cell lines

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MDA-MB-231 cells were obtained from Cell Bank, Type Culture Collection of Chinese Academy of Sciences (Shanghai, China), and maintained in DMEM medium (Gibco, Cat. No.:11965–092) supplemented with 10% fetal bovine serum (FBS, Gibco, Cat. No.: 10099–141) and 1% penicillin & streptomycin (Meilunbio, Cat. No.:MA0110) in a humidified incubator containing 5% CO₂ at 37 °C. MGC803 and HCT8 cells, also obtained from Cell Bank, Type Culture Collection of Chinese Academy of Sciences, were both cultured in RPMI 1640 medium (Meilunbio, Cat. No.: MA 0215). MCF7, gifted by Prof. Tu Hong from Shanghai Jiao Tong University (China), was maintained in DMEM medium supplemented with 10% FBS and 1% penicillin & streptomycin. MCF-10A cells and BT549 cells, obtained from Zhongqiao Xinzhou Biotechnology (Shanghai, China), were cultured in special medium (Cat. No.: ZQ-1311, Zhongqiao Xinzhou Biotechnology) and RPMI 1640 medium, respectively, supplemented with 10% FBS and 1% penicillin & streptomycin.

Jin J., Qiu S., Wang P., Liang X., Huang F., Wu H., Zhang B., Zhang W., Tian X., Xu R., Shi H, & Wu X. (2019). Cardamonin inhibits breast cancer growth by repressing HIF-1α-dependent metabolic reprogramming. Journal of Experimental & Clinical Cancer Research : CR, 38, 377.

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H1N1 Influenza Virus Characterization

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The IAV/FM/1/47 (H1N1) was supplied by the Shanghai Centre for Disease Control & Prevention (Shanghai, China). H1N1 virulence was determined based on its median lethal dose (LD₅₀) of 10^−4.3. The virus was stored in aliquots at −80 °C. The influenza virus was diluted in RPMI 1640 medium (Meilunbio, Dalian, China) until further use.

Hao Y.Z., Cen L.F., Wang T., Yi T., Shi X.L., Duan H.J., Dai Z., Zhu H.Y, & Tang J.G. (2023). The protective effect of 999 XiaoErGanMao granules on the lungs and intestines of influenza A virus-infected mice. Pharmaceutical Biology, 61(1), 630-638.

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Nanoparticle-Mediated Cancer Therapy

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Four-armed polyethylene glycol 2000 Da (star-PEG) was purchased from Qiyue Biotechnology Co., Ltd (Xi'an, China). CL monomers, Sn(Oct)₂, alvespimycin (17-DMAG) HCl, poly (vinyl pyrrolidone) (PVP, K30), potassium hexacyanoferrate (III) (K₃ [Fe(CN)₆]), and hyaluronic acid (HA, 20 KDa) were bought from Macklin Co. (Shanghai, China). Fetal bovine serum (FBS), RPMI 1640 medium, cell counting kit-8 (CCK-8), calcein AM/PI cell viability assay kit, annexin V-FITC apoptosis detection kit, cell cycle and apoptosis analysis kit and DCFH-DA were also acquired from Meilun Biotechnology Co. (Dalian, China). The cyclin A2 rabbit monoclonal antibody, Akt, phospho-Akt (Ser 473), HSP90-1α, HSP70 and GPX4 rabbit polyclonal antibody were purchased from the Beyotime Biotechnology Co. (Haimen, China). ACSL4 mouse monoclonal antibody were bought from Sigma-Aldrich Co. (USA).

Lian H., Guan P., Tan H., Zhang X, & Meng Z. (2021). Near-infrared light triggered multi-hit therapeutic nanosystem for tumor specific photothermal effect amplified signal pathway regulation and ferroptosis. Bioactive Materials, 9, 63-76.

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Cytotoxic and Apoptotic Effects of CTX

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Fetal bovine serum and RPMI-1640 medium were purchased from Dalian Meilun Biology Technology Co., Ltd (Dalian, China). CTX was purchased from Shanxi Powerdone Pharmaceutics Co., Ltd (Shanxi, China). Antibodies of Bax, Bcl-2, cleaved Caspase 3, Fas, FasL, TNF-α, IL-2, Beclin 1, p53, LC3B and CD34 were purchased from Wuhan Servicebio Technology Co., Ltd (Wuhan, China). Elisa kits for murine IL-2, IL-4, IL-6, IL-13, IFN-γ, TNF-α, cleaved Caspase 3, cleaved caspase 8, cleaved Caspase 9, EGFR, Beclin 1, AMPK, PTEN, PI3K, AKT and m-TOR and BCA total protein kit were purchased from Shanghai Yuan-Mu Biomedical Company (Shanghai, China). Kits for determining ALT, AST, GSH-Px, SOD and MDA were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). All other reagents were of highest purity and commercially available by Shenyang Lab Science and Trade Co., Ltd (Shenyang, China). H22 tumor cell line (H8D8) was obtained from associate Prof. Ming-yu Xia of Department of Pharmacological in Shenyang Pharmaceutical University (Shenyang, China).

Zhai J., Song Z., Chang H., Wang Y., Han N., Liu Z, & Yin J. (2021). He-Wei Granule enhances anti-tumor activity of cyclophosphamide by changing tumor microenvironment. Chinese Herbal Medicines, 14(1), 79-89.

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Gastric Cancer Cell Line Cultivation

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Gastric cancer cell lines MKN-45 and SNU-1 were obtained from Cell Resource Center, Institute of Basic Medicine, Chinese Academy of Medical Sciences. HEK293T cell line was purchased from American Type Culture Collection, Manassas, VA, USA. All cell lines were cultured in RPMI 1640 medium (Meilunbio, Dalian, China) with 10% FBS (Biochannel, Nanjing, China) and 1% penicillin/streptomycin (Beyotime, Beijing, China). The chemical PR-619 (Cat. No. HY-13814) was purchased from MedChemExpress, New Jersey, USA. The primary gastric cancer tissues were received from the First Affiliated Hospital of Soochow University, and the case information was listed in the Supplementary Table 1. The collection and use of human gastric cancer tissues for this study were approved by the Review and Ethics committee of the First Affiliated Hospital of Soochow University.

Tao Y, & You W. (2022). The Deubiquitinating Enzyme USP4 Functions as an Oncoprotein in Gastric Cancer and Mediates NF-κB Signaling by Regulating PRL-3 Expression. Frontiers in bioscience (Landmark edition), 27(10).

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Cell Culture of Gastric Cancer Lines

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GES1, HGC-27, and MGC-803 cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA) and stored at the Shanghai Institute of Digestive Surgery. All the three cell lines were cultured in RPMI-1640 medium (Meilunbio, China) supplemented with 10% fetal bovine serum (Sunrise, Uruguay) in a humidified atmosphere at 37°C with 5% CO₂.

Shao Y., Jia H., Li S., Huang L., Aikemu B., Yang G., Zhang S., Sun J, & Zheng M. (2021). Comprehensive Analysis of Ferroptosis-Related Markers for the Clinical and Biological Value in Gastric Cancer. Oxidative Medicine and Cellular Longevity, 2021, 7007933.

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