Cellsens system

Manufactured by Olympus

Sourced in United States

The CellSens system is a software platform designed for microscope image acquisition, processing, and analysis. It provides a comprehensive set of tools for researchers and scientists working with cell and tissue samples.

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Lab products found in correlation

Z0334, by Agilent Technologies (2 mentions) Neuronal nuclei (neun), by Merck Group (2 mentions) Mab337, by Merck Group (2 mentions) Streptavidin hrp and dab chromogen, by Thermo Fisher Scientific (1 mentions) Szx16, by Olympus (1 mentions) Escherichia coli, by Merck Group (1 mentions) 4 6 diamidino 2 phenylindole dapi, by Abcam (1 mentions) Anti inos antibody, by Abcam (1 mentions)

Close spelling variants of this product

CellSens (265 citations) CellSens Standard electronic system (18 citations) CellSens Dimension system (8 citations) CellSens Standard system (5 citations) CellSens Imaging System (5 citations) CellSens Entry system (3 citations) CellSens Dimension Imaging System (2 citations) CellSens image analysis system (2 citations) CellSens Dimention imaging system (2 citations) CellSens Entry 1.9 imaging system (2 citations)

5 protocols using cellsens system

Paraffin-Embedded Immunohistochemistry for Thyroid Organoids

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For the H&E or immunohistochemical staining, tissues were fixed overnight in 4% paraformaldehyde and processed for paraffin embedding. The thyroid organoids were collected gently and fixed in 4% PFA solution for 2 h, then embedded in 3% agarose gel before paraffin embedding process. Subsequently, 5‐µm‐thick sections were mounted on slides.
After deparaffinization and rehydration, sections were subjected to antigen retrieval and endogenous peroxidases were quenched, and then blocked with 10% BSA in PBS for 1 h at room temperature. Primary antibodies were then applied at the appropriate dilutions and incubated overnight at 4 °C in a humidified chamber. Next, the biotinylated secondary antibodies α‐mouse IgG or α‐rabbit IgG (Invitrogen, 1:500) was added and incubated for 1 h, followed by the detection with streptavidin‐HRP and DAB chromogen (Invitrogen) according to the manufacturer's recommendations. Finally, slides were counterstained with Mayer's hematoxylin, dehydrated, and mounted. Images were taken by cellSens system of Olympus microscope.

Liang J., Qian J., Yang L., Chen X., Wang X., Lin X., Wang X, & Zhao B. (2022). Modeling Human Thyroid Development by Fetal Tissue‐Derived Organoid Culture. Advanced Science, 9(9), 2105568.

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Immunohistochemical Analysis of Mouse Brain

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Following MR acquisition, the mice (n ≥ 2, each group) were put under general anesthesia and retrograde-perfused with ice-cold saline. Whole brains were removed from perfused animals and frozen in n-butanol on dry ice. The frozen brain tissue sections (thickness, 20 µm) were prepared and stored at −70°C before staining. The brain samples were fixed in 4% paraformaldehyde (PFA, 50 ml) in 0.1 M phosphate buffer (0.1 M Na₂HPO₄ /NaH₂PO₄ pH 7.4) for 10 min, and then were washed in buffer. For antigen detection, the samples were stained overnight with rabbit polyclonal antibodies against GFAP (Z0334, Dako), G-CSF (NBP1-89894, Novous Biologicals), GFP (Z0334A11122, Life Technologies), or NeuN (MAB337, Millipore); all at 1/1000 dilution at 4°C, followed by fluorescein isothiocyanate (FITC)-goat anti-rabbit IgG (1/1000). Vascular endothelia were stained with Cy3-griffonia simplicifolia lectin I (GSL-I, 1/100 dilution), and nucleic acids were stained with Hoechst dye. All histological images were acquired using the same exposure time and gain, using a cellSens system (Olympus USA).

Ren J., Chen Y.I., Liu C.H., Chen P.C., Prentice H., Wu J.Y, & Liu P.K. (2015). Noninvasive Tracking of Gene Transcript and Neuroprotection after Gene Therapy. Gene therapy, 23(1), 1-9.

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Bending Rate Measurements in C. elegans

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For bending measurements, Day 1 adult animals (3 days after synchronization) were transferred to NGM plates containing FUdR seeded with E. coli OP50, L. acidophilus 1244, or L. paracasei subsp. paracasei 2004 for 3 days. After Day 4 of adulthood, animals were transferred and cultured on NGM plates seeded with E. coli OP50 until Day 10 of adulthood. Then, the animals’ bending rate in M9 buffer was recorded under an optical microscope (Olympus SZX16 with CellSens system) for 30 s. The number of bends was counted manually afterward.

Kishimoto S., Nono M., Makizaki Y., Tanaka Y., Ohno H., Nishida E, & Uno M. (2024). Lactobacillus paracasei subsp. paracasei 2004 improves health and lifespan in Caenorhabditis elegans. Scientific Reports, 14, 10453.

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Macrophage Activation Assay using LPS

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LPS obtained from biofilm-forming PAO1, planktonic PAO1, or Escherichia coli (Sigma–Aldrich) was added to PMA-differentiated THP-1 cells at a concentration of 100 ng ml⁻¹. After 5 h, the cells were fixed in 4 % paraformaldehyde for 15 min at room temperature. The cells were then treated with 3 % bovine serum albumin (BSA) for 30 min at 37 °C to block nonspecific staining, and incubated overnight at 4 °C with an anti-iNOS antibody (Abcam). The next day, the cells were rinsed with PBS, and then incubated for 1 h at room temperature with a horseradish peroxidase (HRP)-conjugated IgG antibody (Abcam). Nuclei were counterstained with DAPI (4′,6-diamidino-2-phenylindole, Abcam). The cells were evaluated under an Olympus microscope (CellSens system).

Wang S., Xiang D., Tian F, & Ni M. (2021). Lipopolysaccharide from biofilm-forming Pseudomonas aeruginosa PAO1 induces macrophage hyperinflammatory responses. Journal of Medical Microbiology, 70(4), 001352.

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Immunohistochemical Analysis of Mouse Brain

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Ren J., Chen Y.I., Liu C.H., Chen P.C., Prentice H., Wu J.Y, & Liu P.K. (2015). Noninvasive Tracking of Gene Transcript and Neuroprotection after Gene Therapy. Gene therapy, 23(1), 1-9.

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