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Spectral domain ophthalmic imaging system

Manufactured by Bioptigen
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The Spectral Domain Ophthalmic Imaging System is a lab equipment product designed for high-resolution imaging of the eye. It utilizes spectral domain optical coherence tomography (SD-OCT) technology to capture detailed 3D images of the retina, optic nerve, and other ocular structures.

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Lab products found in correlation

Envisu r2200, by Bioptigen (5 mentions) Invivovue diver 2, by Bioptigen (2 mentions) Systane ultra, by Alcon (1 mentions) Invivovue clinic, by Bioptigen (1 mentions) Invivovue clinic software, by Bioptigen (1 mentions) Recugel, by Bausch & Lomb (1 mentions) Aim ras, by Bioptigen (1 mentions)

9 protocols using spectral domain ophthalmic imaging system

1

Retinal OCT Imaging in Mice

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Mice were anesthetized, and their pupils dilated as described above. Artificial tears (Systane Ultra, Alcon, Fort Worth, TX) were used to maintain corneal hydration and clarity. OCT images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (Bioptigen Envisu R2200, Morrisville, NC). Image acquisition software was provided by the vendor. The thickness of the ONL was measured at a distance of 0.624 mm from the optic nerve head (ONH) using software provided by vendor.
Li Y., Furhang R., Ray A., Duncan T., Soucy J., Mahdi R., Chaitankar V., Gieser L., Poliakov E., Qian H., Liu P., Dong L., Rogozin I.B, & Redmond T.M. (2019). Aberrant RNA splicing is the major pathogenic effect in a knock-in mouse model of the dominantly inherited c.1430A>G human RPE65 mutation. Human mutation, 40(4), 426-443.
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2

Optical Coherence Tomography of Retinal Detachment

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The mice were anesthetized with an intraperitoneal injection of a mixture of ketamine (100 mg/kg) and xylazine (20 mg/kg), and were kept warm on a heating pad. The pupils were dilated with topical tropicamide. A rectangular volume scan, focusing on the injection site and the bleb formed by retinal detachment, was applied using a spectral domain optical coherence tomography (SDOCT) system (Bioptigen Spectral Domain Ophthalmic Imaging System; Bioptigen, Durham, NC). A rectangular, or raster volume scan composed of a series of parallel B-scans, served as an annular cross-sectional view around the point of interest in the tissue. Finally, the imaging and data were exported and analyzed.
Huang R., Baranov P., Lai K., Zhang X., Ge, J, & Young M.J. (2014). Functional and morphological analysis of the subretinal injection of human retinal progenitor cells under Cyclosporin A treatment. Molecular Vision, 20, 1271-1280.
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3

Spectral Domain OCT Imaging of Murine Retina

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SD-OCT was performed using a Bioptigen Spectral Domain Ophthalmic Imaging System (Bioptigen, Inc., Durham, NC, USA) as previously described.28 (link) The system has a platform designed for easy orientation and aligning of mice for retinal imaging, and provides a high resolution of 2 μm. The mice were anesthetized by intraperitoneal injection with a mixed solution of ketamine and xylazine. The pupils were dilated with 1% tropicamide eye drops prior to imaging. Radial volume scan (centered on the optic disc, consisting of 100 B-scans) was acquired using image analysis software (InVivoVue Clinic; Bioptigen, Durham, NC, USA). Outer nuclear layer (ONL) thickness is an indicator of photoreceptor survival, and measurements were assessed using ImageJ (National Institutes of Health) software29 (link) on four selected scans (scan numbers 1, 26, 51, 76 represent 0°, 45°, 90°, and 135°, respectively, in en face images).30 (link) The lines defining ONL (between the outer plexiform layer to the outer limiting membrane) were drawn in ImageJ (2 μm/pixel), one on each side of the optic disc, 300 μm from the optic disc. The measurements of ONL thickness were performed by experienced investigators in a masked fashion.
Xiao J., Adil M.Y., Chang K., Yu Z., Yang L., Utheim T.P., Chen D.F, & Cho K.S. (2019). Visual Contrast Sensitivity Correlates to the Retinal Degeneration in Rhodopsin Knockout Mice. Investigative Ophthalmology & Visual Science, 60(13), 4196-4204.
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4

Optical Coherence Tomography of Mouse Retina

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Spectral domain optical coherence tomogram images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Envisu R2200; Bioptigen, Inc., Morrisville, NC). Mice were anesthetized, and pupils were dilated by applying one or two drops of topical 0.5% tropicamide (Visufarma, Rome, Italy). To prevent corneal desiccation during the procedure, topical lubricant eye drops (Recugel; Bausch & Lomb, Rochester, NY) were applied bilaterally with a small brush. Mice were positioned into the animal imaging mount and rodent alignment stage (AIM-RAS; Bioptigen, Inc.). The laser source was placed in front of the mouse, and images were acquired by the InVivoVue Clinic software (Bioptigen, Inc.). Three images—one central, one superior, and one inferior to the optic nerve—were taken from each eye. Outer nuclear layer thickness was manually measured three times from each OCT scan image and averaged.
Maddalena A., Dell'Aquila F., Giovannelli P., Tiberi P., Wanderlingh L.G., Montefusco S., Tornabene P., Iodice C., Visconte F., Carissimo A., Medina D.L., Castoria G, & Auricchio A. (2018). High-Throughput Screening Identifies Kinase Inhibitors That Increase Dual Adeno-Associated Viral Vector Transduction In Vitro and in Mouse Retina. Human Gene Therapy, 29(8), 886-901.
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5

Quantitative Retinal Imaging in Mice

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Rd10-non, rd10-SA4503, rd10-PRE084, and rd10-(+)-PTZ mice were anesthetized with ketamine/xylazine. The Bioptigen Spectral Domain Ophthalmic Imaging System (Bioptigen Envisu R2200, NC) was used to examine retinal architecture in mice at P42.39 (link),40 Imaging included averaged volume intensity scans centered on the optic nerve head and single B scans. Owing to significant outer retinal disruption in rd10 mice, we used the manual caliper feature to measure total retinal thickness (TRT), the inner retina (from upper edge of inner limiting membrane to lower edge of inner nuclear layer [INL]), outer retina (from lower INL edge to inferior RPE boundary).
Wang J., Xiao H., Barwick S.R, & Smith S.B. (2020). Comparison of Sigma 1 Receptor Ligands SA4503 and PRE084 to (+)-Pentazocine in the rd10 Mouse Model of RP. Investigative Ophthalmology & Visual Science, 61(13), 3.
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6

Retinal Imaging in Rodents

Cited 2 times
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Mice were imaged using a Spectralis OCT as previously described (45 (link)). Rats were imaged using the Bioptigen Spectral-domain ophthalmic imaging system, as previously described (39 (link)). Briefly, image acquisition was obtained by using the rectangular scanning protocol consisting of a 2 mm × 2 mm perimeter with 750 A-scans (lines) and 5 B-scans (frames) with 60 frames/B-scan (for rat retina) or 1.4 mm × 1.4 mm perimeter with 750 A-scans (lines) and 10 B-scans (frames) with 20 frames/B-scan (for mouse retina). The Bioptigen InVivoVue Diver 2.0 was used to enable manual segmentation of the retinal layers and the ONL thickness was measured after exporting results from Diver to Excel. Statistical analysis was performed using two-tailed Student’s t-test for samples with unequal variance, except for the spider plots, which were analyzed by two-way analysis of variance with Sidak correction.
Aguilà M., Bellingham J., Athanasiou D., Bevilacqua D., Duran Y., Maswood R., Parfitt D.A., Iwawaki T., Spyrou G., Smith A.J., Ali R.R, & Cheetham M.E. (2020). AAV-mediated ERdj5 overexpression protects against P23H rhodopsin toxicity. Human Molecular Genetics, 29(8), 1310-1318.
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7

Optical Coherence Tomography Imaging of SRD in Mice

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Optical coherence tomography imaging was carried out in a manner described in Chen et al.25 (link) Briefly, SD-OCT images were obtained in mice observed to have developed SRD using the Bioptigen Spectral Domain Ophthalmic Imaging System (Bioptigen Envisu R2200, Morrisville, NC, USA). Image acquisition software was provided and supported by the vendor. Averaged single B-scan and volume scans were obtained using the optic nerve head (ONH) as an anatomical landmark for orientation.
Luna G., Lewis G.P., Linberg K.A., Chang B., Hu Q., Munson P.J., Maminishkis A., Miller S.S, & Fisher S.K. (2016). Anatomical and Gene Expression Changes in the Retinal Pigmented Epithelium Atrophy 1 (rpea1) Mouse: A Potential Model of Serous Retinal Detachment. Investigative Ophthalmology & Visual Science, 57(11), 4641-4654.
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8

Retinal Architecture Assessment via SD-OCT

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To assess retinal architecture in situ, spectral domain- optical coherence tomography (SD-OCT) was performed. Mice were anesthetized using intraperitoneal injection (i.p.) of rodent anesthesia cocktail (Ketamine 85mg/cc, Xylazine 10 mg/cc) at a dosage of 1 μl/g body weight. Anesthetized mice were wrapped in soft tissue paper and placed within the mouse holding cassette, pupils were dilated with 1% tropicamide. 0.3% hypromellose eye gel (Genteal, Alcon Corp., Fort Worth, TX) was used throughout the procedure to maintain corneal moisture and clarity. Additionally, 0.4% polyethylene glycol 400 (Systane Ultra, Alcon Corp) was applied liberally to maintain corneal moisture during imaging. The SD-OCT images were obtained using the Bioptigen Spectral Domain Ophthalmic Imaging System (SDOIS; Bioptigen Envisu R2200, North Carolina) equipped with the rodent alignment stage and mouse retina probe using proprietary InVivoVue™ Diver 2.4 software (Bioptigen). The Three repeated volume intensity projections, centered on the optic nerve head, were acquired for each eye. The images consisted of 100 averaged B-scans. Images were acquired within 20 minutes after anesthesia administration to ensure that all three scans were acquired rapidly to avoid the development of reversible cataracts.
Huang L., Xiao H., Xie X., Hu F., Tang F., Smith S.B, & Gan L. (2022). Generation of Sigmar1 conditional knockout mouse using CRISPR-Cas9 gene targeting. Genesis (New York, N.Y. : 2000), 60(6-7), e23487.
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9

Retinal Imaging After Intravitreal Injection

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An SD-OCT system (Bioptigen Spectral Domain Ophthalmic Imaging System; Durham, NC, USA) was used to assess the retinal structure post-intravitreal injection in each rabbit at the specified time periods. Rabbits were anesthetized and pupils were dilated with tropicamide, 1% solution. Corneas were lubricated frequently (Systane Ultra ophthalmic lubricant; Alcon Ltd., Fort Worth, TX, USA) during the imaging session. Rabbits were placed in a restrainer for stability, and a pediatric probe was used for OCT analysis. We used 6-mm horizontal line scans in the inferior retina below the optic disk for capturing retinal cross sections.
Li H., Palamoor M, & Jablonski M.M. (2016). Poly(ortho ester) nanoparticles targeted for chronic intraocular diseases: ocular safety and localization after intravitreal injection. Nanotoxicology, 10(8), 1152-1159.
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