Recombinant HDGF protein was generated as previously described [12 (link)]. The following reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA): chetomin (C9623), Bay 11–7082 (B5556), Ponceau S solution (P7170), and β-actin (A5441). The following antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA): VEGF (sc-152), p-AKT (sc-33,437), AKT (sc-1619), p-IκB (sc-8404), p65 (sc-372), STAT3 (sc-482) and the nucleolin neutralizing antibody (sc-8031). Other antibodies were obtained as follows: p-STAT3 (4113; Cell Signaling Technology, Inc., Danvers, MA, USA), IκB (ab32518; Abcam plc., Cambridge, UK), and HIF-1α (NB100–479; Novus International Inc., St Louis, MO, USA).
Iκb ab32518
Manufactured by Abcam
Sourced in United States
IκB (ab32518) is a recombinant rabbit monoclonal antibody that recognizes the IκB protein. IκB is a regulatory protein that inhibits the NF-κB transcription factor, playing a key role in the regulation of the immune response and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
P stat3, by Cell Signaling Technology (1 mentions)
Vegf sc 152, by Santa Cruz Biotechnology (1 mentions)
Bay 11 7082 b5556, by Merck Group (1 mentions)
Chetomin c9623, by Merck Group (1 mentions)
P iκb, by Santa Cruz Biotechnology (1 mentions)
Stat3, by Santa Cruz Biotechnology (1 mentions)
P akt ser473, by Cell Signaling Technology (1 mentions)
Ecl detection kit, by Thermo Fisher Scientific (1 mentions)
Melatonin, by Merck Group (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
β catenin, by Cell Signaling Technology (1 mentions)
Ubiquitin, by Cell Signaling Technology (1 mentions)
P gsk3β ser9, by Cell Signaling Technology (1 mentions)
Lamin a c, by Cell Signaling Technology (1 mentions)
Gsk3β, by Cell Signaling Technology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
P β catenin, by Cell Signaling Technology (1 mentions)
2 protocols using iκb ab32518
1
Recombinant HDGF Protein Characterization
2
Melatonin Modulates EMT Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Melatonin and Lipopolysaccharide (Escherichia coli LPS, serotype 0127:B8) were purchased from Sigma Chemical Co. (St Louis, MO). Antibodies against E-cadherin (#14472), Vimentin (#5741), N-cadherin (#13116), Snail (#3895), Slug (#9585), Twist (#46702), β-catenin (#8480), p-β-catenin (#9561), AKT (#4691), p-AKTSer473 (#4060), GSK-3β (#5676), p-GSK3βSer9 (#9323), p-STAT3 (#9145) β-actin (#4970), Ubiquitin (#3933) and Lamin A/C (#2032) were purchased from Cell Signaling Technology (Beverley, MA). Zeb1 (#ab203829), Axin (#ab233652), and I-κB (#ab32518) antibodies were purchased from Abcam (Cambridge, MA). Antibodies against p-IκB (#sc8404) and STAT3 (#sc8019) were from Santa Cruz Biotechnologies (Santa Cruz, CA). ECL detection kit was from Pierce Biotechnology (Rockford, IL). All the other reagents were purchased from Sigma or as indicated in the specified method.
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