A431 human epidermoid carcinoma

Manufactured by European Collection of Cell Cultures

Sourced in United Kingdom

A431 is a human epidermoid carcinoma cell line derived from the skin. It is a widely used in vitro model for studying various aspects of cancer biology, such as cell signaling, proliferation, and drug response.

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Lab products found in correlation

Passive lysis buffer (plb), by Promega (3 mentions) Vectashield mounting medium containing 4 6 diamidino 2 phenylindole dapi, by Vector Laboratories (2 mentions) T98g glioblastoma, by European Collection of Cell Cultures (2 mentions) Pharmingen fluorescein isothiocyanate fitc annexin 5 apoptosis detection kit 1, by BD (2 mentions) Endotoxin free giga plasmid kit, by Qiagen (2 mentions) T98g human glioblastoma, by European Collection of Cell Cultures (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) 1640 cell culture media, by Thermo Fisher Scientific (1 mentions) Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Cholesterol, by Merck Group (1 mentions) Lactoferrin, by Merck Group (1 mentions) Porf9 mtnfα, by InvivoGen (1 mentions) Pcmvsport β galactosidase, by InvivoGen (1 mentions) Quanti it picogreen dsdna reagent, by Thermo Fisher Scientific (1 mentions) Plumbagin, by Merck Group (1 mentions)

4 protocols using a431 human epidermoid carcinoma

Plumbagin-Based Nanoformulation for Cancer Therapy

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Plumbagin (5‐hydroxy‐2‐methyl‐1,4‐naphthoquinone), human holo‐transferrin (Tf), hydrogenated phosphatidylcholine, cholesterol, and all other chemicals that are not specifically mentioned below were purchased from Sigma Aldrich (Poole, UK). 1,2‐Distearoyl‐sn‐glycero‐3‐phosphoethanolamine‐N‐(carbonyl‐methoxypolyethyleneglycol 2000), sodium salt (DSPE‐PEG) came from NOF Corporation (Tokyo, Japan). cholesterol‐PEG5000‐maleimide was obtained from Nanocs (New York, NY).
A431 human epidermoid carcinoma and T98G glioblastoma were purchased from the European Collection of Cell Cultures (Salisbury, UK), while Bioware^® B16‐F10‐luc‐G5 mouse melanoma that expresses the firefly luciferase was obtained from Caliper Life Sciences (Hopkinton, MA). Dulbecco's modified Eagle medium and Roswell Park Memorial Institute (RPMI) 1640 cell culture media, foetal bovine serum, l‐glutamine, and penicillin–streptomycin were purchased from Life Technologies (Paisley, UK). Passive lysis buffer was obtained from Promega (Southampton, UK). Vectashield^® mounting medium containing 4′,6‐diamidino‐2‐phenylindole (DAPI) was obtained from Vector Laboratories (Peterborough, UK). BD Pharmingen^® fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I was purchased from BD Biosciences (Franklin Lakes, NJ).

Sakpakdeejaroen I., Somani S., Laskar P., Mullin M, & Dufès C. (2019). Transferrin‐bearing liposomes entrapping plumbagin for targeted cancer therapy. Journal of Interdisciplinary Nanomedicine, 4(2), 54-71.

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Transfection with Dendrimer and Cytokine Plasmids

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Lactoferrin and lactoferricin, generation 3-diaminobutyric polypropylenimine dendrimer (DAB) and the other chemicals were purchased from Sigma Aldrich (Poole, UK). The expression plasmids encoding Tumor necrosis factor (TNF) α (pORF9-mTNFα) and β-galactosidase (pCMVsport β-galactosidase) were obtained respectively from InvivoGen (San Diego, CA) and Invitrogen (Paisley, UK) and were purified using an Endotoxin-free Giga Plasmid Kit (Qiagen, Hilden, Germany). Passive lysis buffer was from Promega (Southampton, UK). Quanti-iT™ PicoGreen^® dsDNA reagent and tissue culture media were obtained from Invitrogen (Paisley, UK). Bioware^® B16-F10-luc-G5 mouse melanoma was obtained from Caliper Life Sciences (Hopkinton, MA). A431 human epidermoid carcinoma and T98G human glioblastoma were purchased from the European Collection of Cell Cultures (Salisbury, UK).

Lim L.Y., Koh P.Y., Somani S., Al Robaian M., Karim R., Yean Y.L., Mitchell J., Tate R.J., Edrada-Ebel R., Blatchford D.R., Mullin M, & Dufès C. (2015). Tumor regression following intravenous administration of lactoferrin- and lactoferricin-bearing dendriplexes. Nanomedicine, 11(6), 1445-1454.

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Plumbagin-loaded PLGA Nanocarriers

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Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone, from Plumbago indica), hydrogenated phosphatidylcholine (HPC), human holo-transferrin (Tf), Resomer^® RG 503 H (acid-terminated poly(lactide-co-glycolide) (PLGA-COOH), lactide: glycolide 50:50, viscosity 0.32–0.44 dL/g, MW 24 000–38 000 Da) and all other chemicals not specifically mentioned below were purchased from Sigma Aldrich (Poole, UK). 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide (polyethylene glycol)-2000] (DSPE-PEG2K-MAL) came from Jenkem Technology (Plano, TX). Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute 1640 (RPMI-1640) cell culture media, fetal bovine serum (FBS), L-glutamine and penicillin-streptomycin were purchased from Invitrogen (Paisley, UK). A431 human epidermoid carcinoma and T98G glioblastoma were obtained from the European Collection of Cell Cultures (Salisbury, UK), while Bioware^® B16-F10-luc-G5 mouse melanoma expressing the firefly luciferase was purchased from Caliper Life Sciences (Hopkinton, MA). BD Pharmingen^® Fluorescein isothiocyanate (FITC) Annexin V Apoptosis Detection Kit I was obtained from BD Biosciences (San Jose, CA).

Sakpakdeejaroen I., Somani S., Laskar P., Mullin M, & Dufès C. (2021). Regression of Melanoma Following Intravenous Injection of Plumbagin Entrapped in Transferrin-Conjugated, Lipid–Polymer Hybrid Nanoparticles. International Journal of Nanomedicine, 16, 2615-2631.

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Dendrimer-PEG Conjugates for Gene Delivery

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Generations 3-, 4- and 5- diaminobutyric polypropylenimine (DAB) dendrimers were purchased from Symo-Chem (Eindhoven, The Netherlands). Methoxy PEG succinimidyl carboxymethyl esters of various molecular weights (2 kDa, 5 kDa and 10 kDa) came from Jenkem Technology (Plano, TX, USA). The expression plasmid encoding β-galactosidase (pCMVsport β-galactosidase) was obtained from Invitrogen (Paisley, UK). It was purified using an Endotoxin-free Giga Plasmid Kit (Qiagen, Hilden, Germany). Quanti-iT PicoGreen dsDNA reagent and tissue culture media were purchased from Life Technologies (Paisley, UK). Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) was obtained from Vector Laboratories (Peterborough, UK). Label IT Fluorescein Nucleic Acid Labelling Kit was from Cambridge Biosciences (Cambridge, UK). Passive lysis buffer was obtained from Promega (Southampton, UK). All other materials were purchased from Sigma Aldrich (Poole, UK). Bioware PC-3M-luc-C6 human prostate adenocarcinoma and Bioware B16-F10-luc-G5 mouse melanoma were obtained from Caliper Life Sciences (Hopkinton, MA, USA), while A431 human epidermoid carcinoma, T98G human glioblastoma and DU145 human prostate carcinoma were purchased from the European Collection of Cell Cultures (Salisbury, UK).

Somani S., Laskar P., Altwaijry N., Kewcharoenvong P., Irving C., Robb G., Pickard B.S, & Dufès C. (2018). PEGylation of polypropylenimine dendrimers: effects on cytotoxicity, DNA condensation, gene delivery and expression in cancer cells. Scientific Reports, 8, 9410.

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