HEK293T cells, PK-15 cells, and BHK-21 cells were purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA). Stable PK-15 cells ectopically expressing PRV UL13 (UL13-PK-15 cells), along with control cells, were generated previously [24 (link)]. Cells were maintained in Dulbecco’s minimal essential medium (DMEM) (Gibco, New York, NY, USA) containing 10% fetal bovine serum (FBS) (Biological Industries, Israel) and 1% penicillin–streptomycin (Gibco, New York, NY, USA) at 37 °C in 5% CO2. All cells tested negative for mycoplasma using a mycoplasma detection kit (TransGen Biotech, Beijing, China). PRV (Bartha-K61 strain) was purchased from the China Veterinary Culture Collection Center (Cat# CVCC AV249, Beijing, China), and was purified in BHK-21 cells. The PRV-∆UL13 recombinant strain was generated previously [24 (link)]. The wild-type PRV (PRV-WT) and PRV-∆UL13 PRV strains were amplified and titrated in PK-15 cells using standard protocols. Sendai virus (SeV), described previously [26 (link)], was propagated in 10-day-old embryonated eggs. SeV titers were then determined via the Reed–Muench method using MDCK cells.
Mycoplasma detection kit
Manufactured by Transgene
Sourced in China
The Mycoplasma Detection Kit is a laboratory equipment product designed to detect the presence of mycoplasma contamination in cell cultures. It provides a reliable and efficient method for the identification of mycoplasma species.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (2 mentions)
Bhk 21 cells, by China Veterinary Culture Collection Center (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Hek293t cell, by American Type Culture Collection (1 mentions)
Bhk 21 cells, by American Type Culture Collection (1 mentions)
Pk 15 cells, by American Type Culture Collection (1 mentions)
Oligomycin a, by MedChemExpress (1 mentions)
Rapamycin, by MedChemExpress (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Solarbio (1 mentions)
Streptomycin, by Solarbio (1 mentions)
Hl 60, by Shanghai Cell Bank (1 mentions)
4 protocols using mycoplasma detection kit
1
Cultivation and Characterization of Cell Lines
2
ESCC Cell Lines Characterization
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Human ESCC cell lines EC109, KYSE450, KYSE140, KYSE510, TE-1, and TE-10 were purchased from the cell bank of Peking Union Medical College (Beijing, China). Cells were cultured in RPMI 1640 medium (Gibco) supplemented with 10% FBS (Gibco) in a humidified incubator with 5% CO2 at 37 °C. All cell lines were confirmed by short-tandem repeat (STR) analysis and no mycoplasma contamination certified by using a Mycoplasma Detection Kit (#FM311–01, TransGen Biotech, China).
3
Culturing Human Cell Lines for Research
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Human embryonic kidney 293T cells and human normal colorectal FHC cells were provided by the Laboratory of Biochemistry and Molecular Biology of Yunnan University (Kunming, China). Human colon cancer cell lines were purchased from the Kunming Cell Bank of the Chinese Academy of Science. HT29, HCT8, SW480, Caco2, HCT116, and RKO cells were all cultured in DMEM (Gibco, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (BI, Kibbutz Beit Haemek, Israel) and 1% penicillin/streptomycin (Gibco), and FHC cells were cultivated in RPMI-1640 medium (Gibco). All cells were cultured at 37 °C in a 5% CO2 humidified environment. Mycoplasma was routinely tested using a myco-plasma detection kit (TransGen Biotech, Beijing, China). Oligomycin A and rapamycin used for cell treatment were obtained from MedChem Express (Shanghai, China).
4
Cell lines for Leukemia and Cancer Research
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The human myeloid leukemia cell lines (K562, HL60, U937), the human liver cancer cell line (Huh7), the human T-lymphocyte Cell Line (Jurkat), and 293T cell line were purchased from Shanghai Cell Bank (Shanghai, Chinese Academy of Sciences). DNA fingerprinting and isozyme tests were used to identify these cell lines. All cell lines were negative for Mycoplasma tested by Mycoplasma Detection Kit (TransGen, FM311). All cell lines were used within 3 months after thawing and detected the Mycoplasma every 3 months. K562, HL60, U937, 293T, Huh7, and Jurkat cells were cultured in RPMI1640 (Hyclone) or DMEM (Hyclone) containing 10% FBS (Gibco), 100 U/mL penicillin, 100 μg/mL streptomycin (Solarbio), and 2 mmol/L GlutaMAX (Gibco). K562, HL60, and U937 cells were stably transfected with luciferase. U937-Ep cells were lentivirally transduced with the full-length human EpCAM gene as the target cells for the EpCAM CAR-T cells. Primary AML cells from the bone marrow of patients with AML were obtained under the approval of the Ethics Committee of the University of Science and Technology of China (2021-N(H)-120; Hefei, China).
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