Factor b

Manufactured by Merck Group

Factor B is a laboratory reagent used in the analysis of biological samples. It is a protein involved in the complement system, which is a part of the immune response. Factor B plays a role in the activation of the alternative pathway of the complement system. This product is intended for research use only and its specific applications should be determined by the user.

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Lab products found in correlation

Bovine serum albumin (bsa), by Merck Group (1 mentions) Ap conjugated rabbit anti goat igg antibody, by Merck Group (1 mentions)

2 protocols using factor b

Quantifying C3 Convertase Regulation by FHR1

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To evaluate the potential effect of FHR1 on the FH-mediated regulation of the C3bBb, C3 convertase assay was performed as previously described (24 (link)). In brief, at first 5μg/ml C3b (Complement Tech) were immobilized in the microtiter plates. After blocking with 3% milk in 0.02%TBST for 2 hours at 25°C, 4μg/ml factor B (Millipore), 8μg/ml properdin (Millipore), 0.2μg/ml factor D (Millipore), 20μg/ml BSA (Sigma-Aldrich), altogether with 100nM factor H (Complement Tech) and serially diluted FHR1*A or FHR1*B (300nM-500nM) were added. After incubation, anti-factor B goat polyclonal antibody (Sigma-Aldrich) was added for incubation, followed by adding AP-conjugated rabbit anti-goat IgG antibody (Sigma-Aldrich). Finally, after incubated with alkaline phosphatase chromogenic substrate, the optical density was read at 405 nm.

Xu B., Kang Y., Du Y., Guo W., Zhu L, & Zhang H. (2022). Atypical Hemolytic Uremic Syndrome-Associated FHR1 Isoform FHR1*B Enhances Complement Activation and Inflammation. Frontiers in Immunology, 13, 755694.

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Complement System Serum Depletion Protocol

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Normal human serum (Serum), as well as the complement series depleted serum (Serum-C1, C2, C3, C5, C6) were obtained from Sigma. Rabbit serum was also obtained from Sigma. Antibody-depleted serum (Serum-Ig), with the control serum were from SCIPAC. Serum depleted of C4 (Serum-C4), factor B (Serum-fB) and factor D (Serum-fD), along with matching control serum was from Quidel. Mouse and cat serum were from Equitech Bio Inc. Heat inactivation was carried out at 52°C for 45 minutes. EGTA treatment was by adding 0.2M EGTA in 0.2M MgCl₂. Purified complement C3, factor B and factor D were from Millipore. Cobra venom factor (CVF) was purified from Naja naja kaouthia venom (MP Biomedicals) using the method of Vogel and Muller-Eberhard(55 (link)). Recombinant human rhinovirus 3C protease was from Expedeon Enzymes, and recombinant poliovirus 3C protease prepared by I. Goodfellow, University of Cambridge. Serum was used at the maximum concentration which had no impact on entry, as found by the endosomal disruption assay (typically 1/5 to 1/10 dilutions in PBS); with depleted serum verified using sheep red blood cell hemolysis assays(56 ).

Tam J.C., Bidgood S.R., McEwan W.A, & James L.C. (2014). Intracellular sensing of complement C3 activates cell autonomous immunity. Science (New York, N.Y.), 345(6201), 1256070.

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