Mice were intraperitoneally (i.p.) anesthetized with a mixture of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, The Netherlands; 0.4 mg/kg). EMKA invasive measurement of dynamic resistance (EMKA Technologies, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, The Netherlands; 0–25 mg/mL, 10% puff for 10 s.) was used on day 14 to assess lung function. Average lung resistance (RL) is presented in cm H2O/(mL/sec) (18 (link)).
Emka invasive measurement of dynamic resistance
Manufactured by EMKA Technologies
Sourced in France
The EMKA invasive measurement of dynamic resistance is a lab equipment product that measures the dynamic resistance of materials or systems. It provides precise data on the resistance properties under varying conditions.
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6 protocols using emka invasive measurement of dynamic resistance
1
Lung Function Measurement in Mice
2
Invasive Lung Function Measurement in Mice
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The EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich) (0–25 mg/ mL, 10% puff for 10 s) was used to measure lung function in anesthetized mice. Data are presented as average lung resistance (RL) in cm H2O/mL*sec-1 (26 (link)).
3
Lung Function Measurement in Lactating Dams
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The EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich) (0–25 mg/mL, 10% puff for 10 s) was used to determine lung function of dams on day 21 of lactation (day 42 of the experiment) as performed previously (Verheijden et al., 2014 (link)). Mice were anesthetized by intraperitoneal injection of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, Netherlands; 0.4 mg/kg) mixture. Data are expressed as average lung resistance (RL) in cm H2O/mL × sec–1.
4
Lung Function Assessment in Mice
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Mice were anaesthetized with a mixture of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, The Netherlands; 0.4 mg/kg), intraperitoneally (i.p.). EMKA invasive measurement of dynamic resistance (EMKA Technologies, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, The Netherlands; 0–25 mg/mL, 10 % puff for 10 s.) was used to determine the lung function on day 14 [13 (link)]. Basal airway resistance values of each individual mouse as measured prior to methacholine exposure were deducted from the resistance as measured upon methacholine exposure (ΔR; Fig. 1 b–c).
5
Murine Asthma Model: Lung Function Assessment
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One day after the final HDM challenge, mice were anesthetized by an intraperitoneal injection with a mixture of ketamine (Vetoquinol S.A., France; 125 mg/kg) and medetomidine (Pfizer, The Netherlands; 0.4 mg/kg). EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich) (0–25 mg/mL, 10% puff for 10 s) was used to determine lung function (35 (link)). This is in line with a previous murine house dust mite-induced asthma model where lung function (lung resistance) was measured 24h after the last HDM challenge (37 (link)). Data are expressed as average lung resistance (RL), dynamic compliance (Cdyn) and pleural pressure (dPpl) in cm H2O/mL*sec-1 and the area under the curve (AUC) as the indexes (40 (link)).
6
Invasive Lung Function Measurement
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Mice were intraperitoneally (i.p.) anaesthetized with a mix containing Ketamine (Vetoquinol S.A., Lure Cedex, France; 125 mg/kg) and Medetomidine (Pfizer, Capelle a/d Ijssel, Netherlands; 0.4 mg/kg). Lung function was assessed using EMKA invasive measurement of dynamic resistance (EMKA Technologies, Paris, France) in response to increasing doses of methacholine (acetyl-β-methyl-choline chloride, Sigma-Aldrich, Zwijndrecht, The Netherlands) (0–25 mg/mL, 10% puff for 10 sec.). Data are presented as average lung resistance (RL) in cm H2O/mL*sec−1 [28 (link)].
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