All of serum samples were stored at −80 °C until use. Sample preparation were performed according to a modified protocol based on Püttmann et al.33 (link). Forty FFAs were analyzed using an ACQUITY ultra-performance liquid chromatography (UPLC) system (Waters Corporation, Milford, USA) equipped with a binary solvent delivery system and an auto-sampler (Waters Corporation, Milford, USA), coupled to a tandem quadrupole-time-of-flight (Q-TOF) mass spectrometry (Waters Corporation, Milford, USA)16 (link). For details, see the Supplementary information . A mixture of all the reference standards at an appropriate concentration was prepared and run after every ten serum samples for quality control. Twenty-four FFAs ratios were calculated by the absolute concentrations of product- to-precursor.
Binary solvent delivery system
Manufactured by Waters Corporation
Sourced in United States
The Binary Solvent Delivery System is a core component of liquid chromatography instrumentation. It is designed to precisely deliver two separate solvents or mobile phases to the system, enabling solvent mixing and gradient elution capabilities for a wide range of analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Acquity ultra performance liquid chromatography system, by Waters Corporation (1 mentions)
Q tof premier, by Waters Corporation (1 mentions)
Uplc system, by Waters Corporation (1 mentions)
Acquity hss t3 column, by Waters Corporation (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
3 protocols using binary solvent delivery system
1
FFA Analysis in Serum Samples
2
UPLC-Q-Tof Mass Spectrometry Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A UPLC system equipped with a binary solvent delivery system was sourced from Waters Corp. (Milford, MA, USA), coupled with an auto-sampler and a UV detector as previously reported [22 (link)]. The quadrupole time-of-flight mass spectrometer (Q-Tof Premier™, Waters Corp., Milford, MA, USA) was used. The lock mass used was a reference solution of leucine-enkephalin ([M − H] − m/z 554.2615) in the form of a spray.
3
Quantification of Triptan Drugs in Milk
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Milk samples, spiked standards and QC samples were extracted as follows: 50 µL sample, 25 µL internal standard solution, 25 µL 0.1 mol/L Na 2 CO 3 (pH 11) and 1 mL n-chlorobutane:acetonitrile (4:1) were mixed and centrifuged. The organic phase was pipetted off, transferred to new tubes and evaporated to dryness under a stream of nitrogen at 40°C, and the remaining extracts were reconstituted in 100 µL water:methanol (95:5) with 0.1% formic acid. 2.4.3 | Liquid chromatography-tandem mass spectrometry Samples were analysed by LC-MS/MS using a Waters Acquity UPLC system with an autosampler and a binary solvent delivery system (Waters) interfaced to Waters Xevo TQ-S benchtop tandem quadrupole mass spectrometer (Waters). Separation was performed on a 2.1 × 100 mm Waters Acquity HSS T3 column maintained at 50°C.
Lower limit of quantification (LLOQ) was found to be 0.25 nM for almotriptan and naratriptan, 0.5 nM for sumatriptan, zolmitriptan and rizatriptan, and 1 nM for eletriptan, N-desmethyleletriptan and N-desmethylzolmitriptan. For a more detailed description of the LC-MS/MS method, preparation of calibrators and controls, and precision and accuracy, see Appendix S1.
Lower limit of quantification (LLOQ) was found to be 0.25 nM for almotriptan and naratriptan, 0.5 nM for sumatriptan, zolmitriptan and rizatriptan, and 1 nM for eletriptan, N-desmethyleletriptan and N-desmethylzolmitriptan. For a more detailed description of the LC-MS/MS method, preparation of calibrators and controls, and precision and accuracy, see Appendix S1.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!