The following chemicals were purchased from Sigma-Aldrich (St. Louis, MO): methanol and acetonitrile (MS grade), ammonium acetate, ascorbic acid, β-mercaptoethanol, formic acid and standards, 5-adenosyl-methionine, 5-adenosyl-homocysteine, ATP, betaine, choline, cyanocobalamin, cystathionine, cysteine, dihydrofolate, dimethylglycine, dUMP, folic acid, folinic acid, glycine, homocysteine, methionine, methylcobalamine, methyl-tetrahydrofolate, NADPH, pyridoxal 5-phosphate, riboflavin, serine, taurine, tetrahydrofolate, thymidine 5-phosphate and Dulbecco's modified Eagle's medium mixed 1:1 with Ham's F-12 (DMEM/F12). Ultrapure type 1 water was obtained from a Milli Q water system (Merck Millipore, Darmstadt, Germany). Matrigel (growth factor-reduced) was purchased from BD Biosciences (San Jose, CA). Recombinant basic fibroblast growth factor (bFGF) was purchased from Mylteni (San Diego, USA).
Dimethylglycine
Manufactured by Merck Group
Sourced in Macao
Dimethylglycine is a chemical compound used in various laboratory applications. It is a form of glycine, an amino acid, with two methyl groups attached. Dimethylglycine serves as a buffer and may be utilized in analytical procedures, biochemical assays, and other laboratory research activities.
Automatically generated - may contain errors
Lab products found in correlation
Serine, by Merck Group (2 mentions)
Betaine, by Merck Group (2 mentions)
Succinate, by Merck Group (2 mentions)
Methionine, by Merck Group (1 mentions)
Cyanocobalamin, by Merck Group (1 mentions)
Homocysteine, by Merck Group (1 mentions)
Riboflavin, by Merck Group (1 mentions)
Choline, by Merck Group (1 mentions)
Folinic acid, by Merck Group (1 mentions)
Cystathionine, by Merck Group (1 mentions)
Dihydrofolate, by Merck Group (1 mentions)
Tetrahydrofolate, by Merck Group (1 mentions)
Taurine, by Merck Group (1 mentions)
Milli q water system, by Merck Group (1 mentions)
Pyridoxal 5 phosphate, by Merck Group (1 mentions)
Methyl tetrahydrofolate, by Merck Group (1 mentions)
Adenosine triphosphate (atp), by Merck Group (1 mentions)
Ammonium acetate, by Merck Group (1 mentions)
Ascorbic acid, by Merck Group (1 mentions)
Folic acid, by Merck Group (1 mentions)
3 protocols using dimethylglycine
1
Metabolite Profiling for Cell Culture
2
Comprehensive Metabolomics Sample Preparation
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Methanol, acetonitrile, perchloric
acid (PCA), trichloroacetic
acid (TCA), hydrochloric acid (HCl), sodium hydroxide (NaOH), (2-bromoethyl)
trimethylammonium bromide, dimethylformamide (DMF), 3-(trimethylsilyl)propionic
acid-2,2,3,3-d4 sodium salt (TSP) were
all obtained from Sigma-Aldrich (St. Louis, MO). 4-(4,6-Dimethoxy[1,3,5]triazin-2-yl)-4-methylmorpholinium
chloride (DMTMM) was obtained from Acros Organic (Pittsburgh, PA),
while 15N-phthalimide potassium and deuterium oxide were
obtained from Cambridge Isotope Laboratories (Andover, MA). All chemicals
were used without further purification. Pooled human serum sample
was obtained from Innovative Research, Inc. (Novi, MI). Deionized
(DI) water was purified using an in-house Synergy Ultrapure Water
System from Millipore (Billerica, MA). Centrifugal filters (3-kDa
cutoff; Amicon Microcon, YM-3) were purchased from Sigma-Aldrich.
Standard compounds used for spiking and confirming the peak assignments
were 1-methylhistidine, 2-hydroxybutyrate, 2-hydroxyisocaproate, 2-hydroxyisovalerate,
2-oxocaproate, 3-methylbutyrate, 3-methylhistidine, 3-methyl-2-oxobutanoate,
arginine, benzoate, betaine, carnitine, citrulline, dimethylglycine,
pyridoxine, pyroglutamate, ornithine, sarcosine, serine and succinate
(all from Sigma-Aldrich).
acid (PCA), trichloroacetic
acid (TCA), hydrochloric acid (HCl), sodium hydroxide (NaOH), (2-bromoethyl)
trimethylammonium bromide, dimethylformamide (DMF), 3-(trimethylsilyl)propionic
acid-2,2,3,3-d4 sodium salt (TSP) were
all obtained from Sigma-Aldrich (St. Louis, MO). 4-(4,6-Dimethoxy[1,3,5]triazin-2-yl)-4-methylmorpholinium
chloride (DMTMM) was obtained from Acros Organic (Pittsburgh, PA),
while 15N-phthalimide potassium and deuterium oxide were
obtained from Cambridge Isotope Laboratories (Andover, MA). All chemicals
were used without further purification. Pooled human serum sample
was obtained from Innovative Research, Inc. (Novi, MI). Deionized
(DI) water was purified using an in-house Synergy Ultrapure Water
System from Millipore (Billerica, MA). Centrifugal filters (3-kDa
cutoff; Amicon Microcon, YM-3) were purchased from Sigma-Aldrich.
Standard compounds used for spiking and confirming the peak assignments
were 1-methylhistidine, 2-hydroxybutyrate, 2-hydroxyisocaproate, 2-hydroxyisovalerate,
2-oxocaproate, 3-methylbutyrate, 3-methylhistidine, 3-methyl-2-oxobutanoate,
arginine, benzoate, betaine, carnitine, citrulline, dimethylglycine,
pyridoxine, pyroglutamate, ornithine, sarcosine, serine and succinate
(all from Sigma-Aldrich).
3
Metabolite Standard Preparation
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Metabolite standards were acquired
from the MS metabolite library of standards (IROA Technologies, Sea
Girt, NJ). Lactate, malate, succinate, fumarate, dimethyl glycine,
benzoic acid, and 4-formylbenzoic acid (4-FBA) were purchased from
Sigma Aldrich (St. Louis, MO). Each metabolite was reconstituted to
100 μM in 5% MeOH, mixed, dried, and reconstituted to create
a 1 μM mixed acid stock in dimethylformamide (DMF). All other
reagents were purchased from Sigma Aldrich unless otherwise noted.
from the MS metabolite library of standards (IROA Technologies, Sea
Girt, NJ). Lactate, malate, succinate, fumarate, dimethyl glycine,
benzoic acid, and 4-formylbenzoic acid (4-FBA) were purchased from
Sigma Aldrich (St. Louis, MO). Each metabolite was reconstituted to
100 μM in 5% MeOH, mixed, dried, and reconstituted to create
a 1 μM mixed acid stock in dimethylformamide (DMF). All other
reagents were purchased from Sigma Aldrich unless otherwise noted.
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