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MS-275 is a laboratory product manufactured by Santa Cruz Biotechnology. It is a chemical compound used for research purposes. The core function of MS-275 is to inhibit histone deacetylase activity, which is a common technique in biochemical research.

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Lab products found in correlation

Octyl r 2hg, by Merck Group (1 mentions) Mocetinostat, by Cayman Chemical (1 mentions) Pronase, by Merck Group (1 mentions) Trichostatin a, by Cayman Chemical (1 mentions) Tmp195, by Cayman Chemical (1 mentions) Rgfp966, by Cayman Chemical (1 mentions) Brd6688, by Cayman Chemical (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Hydroxyurea, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Benzyl isothiocyanate bitc, by Merck Group (1 mentions) Tubastatin a, by Abcam (1 mentions)

4 protocols using ms 275

1

Hematopoietic Induction with (R)-2HG and MS275

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DMSO (solvent control), 0.1 mM Octyl-(R)-2HG (Sigma-Aldrich) or 2 μM MS275 (Santa Cruz) was added for 16 hours to the hematopoietic induction cultures before harvesting the floating cells.
Olofsen P.A., Fatrai S., van Strien P.M., Obenauer J.C., de Looper H.W., Hoogenboezem R.M., Erpelinck-Verschueren C.A., Vermeulen M.P., Roovers O., Haferlach T., Jansen J.H., Ghazvini M., Bindels E.M., Schneider R.K., de Pater E.M, & Touw I.P. (2020). Malignant Transformation Involving CXXC4 Mutations Identified in a Leukemic Progression Model of Severe Congenital Neutropenia. Cell Reports Medicine, 1(5), 100074.
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2

Modulation of HDAC and NLRP3 Pathways

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Pan-HDAC inhibitor Trichostatin A (TSA), class IIa HDAC inhibitors (HDAC4,5,7 and 9) TMP195, Class I & IV HDAC inhibitor (HDAC1,2,3 and 11) Mocetinostat, HDAC6 and 8 inhibitor BRD73954, HDAC3 inhibitor RGFP966 and a HDAC1 and 2 inhibitor BRD6688 were purchased from Cayman Chemical, HDAC1 and 3 inhibitor MS-275, and HDAC1 inhibitor 4-(dimethylamino)-N-[6-(hydroxyamino)-6-oxohexyl]-benzamide (DHOB) were purchased from Santa Cruz Biotechnology, NLRP3 specific inhibitor CP-456773/MCC 950, caspase-1 inhibitor caspase-1 Inhibitor II and protein synthesis inhibitor, cycloheximide, and pronase were purchased from Sigma-Aldrich. The stocks of all the chemical inhibitors were prepared in DMSO following the instructions of the vendors and stored at -80°C before use.
Moreira J.D., Iakhiaev A., Vankayalapati R., Jung B.G, & Samten B. (2022). Histone deacetylase-2 controls IL-1β production through the regulation of NLRP3 expression and activation in tuberculosis infection. iScience, 25(8), 104799.
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3

Hydroxyurea and MS-275 Preparations

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Hydroxyurea (Sigma) was dissolved in water to make a fresh 10mM stock solution just prior to use in each experiment. MS-275 (Santa Cruz) was dissolved to make a 10mM stock solution in DMSO, and diluted in fresh media just prior to use in each experiment. DMSO was added in equivalent concentrations to control samples.
Esrick E.B., McConkey M., Lin K., Frisbee A, & Ebert B.L. (2015). Inactivation of HDAC1 or HDAC2 induces gamma globin expression without altering cell cycle or proliferation. American journal of hematology, 90(7), 624-628.
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4

Sulforaphane and Histone Deacetylase Inhibitors

Cited 1 time
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R,S-Sulforaphane (SFN) was from LKT Laboratories. S-4 was synthesized as described (Zhang et al., 2014 (link)). Benzyl isothiocyanate (BITC) and rapamycin were from Sigma-Aldrich. Tubastatin A was from BioVision. MS-275 and m-carboxycinnamic acid bis-hydroxamide (CBHA) were from Santa Cruz Biotechnology. The compounds were dissolved in DMSO (vehicle) and diluted in the cell culture medium such that the final DMSO concentration was maintained at 0.1%. The concentrations of each compound are stated in the figure legends.
Zhang Y., Gilmour A., Ahn Y.H., de la Vega L, & Dinkova-Kostova A.T. (2021). The isothiocyanate sulforaphane inhibits mTOR in an NRF2-independent manner. Phytomedicine, 86, None.
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