Mechanical sensitivity was determined by obtaining hind paw withdrawal thresholds, similar to what is described in Edwards et al. (2012) (link). Animals were first acclimated for 10 min to individual plexiglass compartments set on top of a mesh stand. A series of nylon von Frey filaments (North Coast Medical) were applied perpendicularly to the plantar surface of the hind paw until they buckled for 2 s, and a sharp withdrawal of the stimulated hind paw before or within the 2 s indicated a positive response. Testing was initiated with the filament corresponding to 15 g of force and continued in accordance with the up-and-down method (Dixon, 1980 (link)). The 50% paw withdrawal threshold was determined by the formula Xf + kδ, where Xf = last von Frey filament used, k = Dixon value corresponding to response pattern, and δ = mean difference between stimuli. Baseline paw withdrawal thresholds were obtained prior to CFA administration and/or alcohol exposure for all experimental groups.
Nylon von frey filaments
Manufactured by North Coast Medical
Sourced in United States
Nylon von Frey filaments are flexible, graded nylon monofilaments used to assess mechanical sensitivity. They are designed to apply a precise amount of force when pressed against the skin or other surfaces.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nylon von frey filaments
1
Measuring Mechanical Sensitivity in Rodents
2
Mechanical Allodynia Assessment in Rats
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Testing was done three and four hours after carrageenan injection. Rats were placed over
a wire mesh platform and covered with plexiglass cages (16 cm × 16 cm × 16 cm). Following
acclimatization for 30 min, mechanical allodynia was evaluated by calibrated nylon von
Frey filaments of different sizes (3.61, 3.84, 4.08, 4.31, 4.56, 4.74, 4.93, and 5.18;
North Coast Medical Inc., San Jose, USA) using the up-down method.20 (link)
The maximum pressure exerted by these filaments varies between 0.4 and 15 g.
Testing was performed at the center of the inflamed region. The behavioral end point was
reflex withdrawal of the right paw, and the pressure (g) designated as the “withdrawal
threshold.” If there was no withdrawal till the filament size of 5.18, the value was
presumed to be 15 g. An algorithm was used to calculate the 50% withdrawal threshold (g).
Higher values of threshold indicate less pain as rats were able to withstand more pressure
before withdrawal.
a wire mesh platform and covered with plexiglass cages (16 cm × 16 cm × 16 cm). Following
acclimatization for 30 min, mechanical allodynia was evaluated by calibrated nylon von
Frey filaments of different sizes (3.61, 3.84, 4.08, 4.31, 4.56, 4.74, 4.93, and 5.18;
North Coast Medical Inc., San Jose, USA) using the up-down method.20 (link)
The maximum pressure exerted by these filaments varies between 0.4 and 15 g.
Testing was performed at the center of the inflamed region. The behavioral end point was
reflex withdrawal of the right paw, and the pressure (g) designated as the “withdrawal
threshold.” If there was no withdrawal till the filament size of 5.18, the value was
presumed to be 15 g. An algorithm was used to calculate the 50% withdrawal threshold (g).
Higher values of threshold indicate less pain as rats were able to withstand more pressure
before withdrawal.
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